AB236251

Anti-Integrin beta 4 antibody

Name: Anti-Integrin beta 4 antibody (ab236251) | Abcam
Brand: Abcam Limited
SKU: AB236251
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Integrin beta 4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGB4 aa 1400-1550.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody (AB236251)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody (AB236251)

Paraffin-embedded human pancreatic cancer tissue stained for Integrin beta 4 with ab236251 at a 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody (AB236251)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody (AB236251)

A549 (Human lung carcinoma cell line) cells stained for Integrin beta 4 (Green) using ab236251 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody (AB236251)

Paraffin-embedded human placenta tissue stained for Integrin beta 4 with ab236251 at a 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Integrin beta 4 antibody (AB236251)

All lanes:

Western blot - Anti-Integrin beta 4 antibody (ab236251) at 1/500 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 202 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITGB4 aa 1400-1550. The exact immunogen used to generate this antibody is proprietary information.

P16144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin Β4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein's influence on carcinogenic processes and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38551497

2024

Subpopulation commensalism promotes Rac1-dependent invasion of single cells via laminin-332.

Applications

Unspecified application

Species

Unspecified reactive species

Sung Bo Yoon,Luxiao Chen,Isaac E Robinson,Tala O Khatib,Robert A Arthur,Henry Claussen,Najdat M Zohbi,Hao Wu,Janna K Mouw,Adam I Marcus

International journal of molecular sciences 24: PubMed37958530

2023

Delivery and Transcriptome Assessment of an In Vitro Three-Dimensional Proximal Tubule Model Established by Human Kidney 2 Cells in Clinical Gelatin Sponges.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yi Hsiao,Tzung-Hai Yen,Fang-Yu Wu,Chao-Min Cheng,Jia-Wei Liu,Yu-Ting Fan,Jung-Ju Huang,Chung-Yi Nien

Cell reports 35:109223 PubMed34107247

2021

Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality.

Applications

Unspecified application

Species

Unspecified reactive species

Svenja C Schüler,Joanna M Kirkpatrick,Manuel Schmidt,Deolinda Santinha,Philipp Koch,Simone Di Sanzo,Emilio Cirri,Martin Hemberg,Alessandro Ori,Julia von Maltzahn

Oncogene 39:3754-3773 PubMed32157214

2020

Identification of Sca-1Abcg1 bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huijing Yin,Zhengyu Jiang,Xu Feng,Zhaodong Ji,Wei Jin

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Integrin beta 4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody (AB236251)

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody (AB236251)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody (AB236251)

Western blot - Anti-Integrin beta 4 antibody (AB236251)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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