Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 23 publications.
Images
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (AB182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img120997.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49787.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107908.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41180.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (AB182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img164139.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)")
Publications
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- Cancer gene therapy 30:414-4232022KCNF1 promotes lung cancer by modulating ITGB4 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChing-Yi Chen,Pei-Ying Wu,Michelle Van Scoyk,Stephanie A Simko,Chu-Fang Chou,Robert A WinnPubMed 36385523
- Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 33:e138712022ITGB4 deficiency induces DNA damage by downregulating HDAC1 in airway epithelial cells under stress stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Xu,Ye Yao,Huijun Liu,Ming Yang,Lin Yuan,Xizi Du,Yu Yang,Ling Qin,Weijie Wang,Kai Zhou,Xinyu Wu,Chi LiuPubMed 36282138
- Cell communication and signaling : CCS 20:1222022Netrin-1 induces the anti-apoptotic and pro-survival effects of B-ALL cells through the Unc5b-MAPK axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Huang,Xizhou An,Yao Zhu,Kainan Zhang,Li Xiao,Xinyuan Yao,Xing Zeng,Shaoyan Liang,Jie YuPubMed 35974411
- Frontiers in medicine 9:9438872022Comparative proteomic and clinicopathological analysis of breast adenoid cystic carcinoma and basal-like triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Yao,Wei Hou,Junbing Chen,Yanhua Bai,Mengping Long,Xiaozheng Huang,Chen Zhao,Lixin Zhou,Dongfeng NiuPubMed 35966872
- Gut and liver 17:412-4292022TCN1 Deficiency Inhibits the Malignancy of Colorectal Cancer Cells by Regulating the ITGB4 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinqiang Zhu,Xuetong Jiang,Qinglin Zhang,Hailong Huang,Xiaohong Shi,Daorong Hou,Chungen XingPubMed 35686504
- Molecular cancer research : MCR 20:1305-13192022KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeo-Hyun Choi,Jin K Kim,Chin-Tung Chen,Chao Wu,Michael R Marco,Francisco M Barriga,Kevin O'Rourke,Raphael Pelossof,Xuan Qu,Qing Chang,Elisa de Stanchina,Jinru Shia,J Joshua Smith,Francisco Sanchez-Vega,Julio Garcia-AguilarPubMed 35394541
- The Journal of cell biology 221:2022PD-LI promotes rear retraction during persistent cell migration by altering integrin β4 dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdie Wang,Choua Xiong,Arthur M MercurioPubMed 35344032
- Frontiers in cell and developmental biology 10:8454402022Deficiency of Integrin 4 Results in Increased Lung Tissue Stiffness and Responds to Substrate Stiffness Modulating RhoA Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinxiu Chi,Yu Chen,Wang Jiang,Wenjie Huang,Mingxing Ouyang,Lei Liu,Yan Pan,Jingjing Li,Xiangping Qu,Huijun Liu,Chi Liu,Linhong Deng,Xiaoqun Qin,Yang XiangPubMed 35309934
- Experimental dermatology 31:413-4192021The adhesive heterogeneity of different compartments of oral mucosal rete ridges.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Li,Heng Chen,Sangang He,Guoliang SaPubMed 34665894
- Journal of Cancer 12:5622-56322021NEAT1/miR-101-dependent Up-regulation of DNA-PKcs Enhances Malignant Behaviors of Pancreatic Ductal Adenocarcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Hu,Wuqiang Chen,Shuo Zhang,Yuzheng Xue,Youzhao He,Yuanlong GuPubMed 34405022
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).
Alternative names
Integrin beta-4, GP150, ITGB4
Recommended products
Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 23 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR17517
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img120997.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
Lane 2: ITGB4 knockout HAP1 whole cell lysate (40 μg)Lanes 1 - 2: Merged signal (red and green). Green - ab182120 observed at 240 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab182120 was shown to specifically recognize ITGB4 in wild-type HAP1 cells alond with additional cross-reactive bands. No band was observed when ITGB4 knockout samples were used. Wild-type and ITGB4 knockout samples were subjected to SDS-PAGE. ab182120 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Predicted band size: 202 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49787.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107908.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cell junction staining on mouse pancreatic acinus is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41180.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human colon epithelium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img164139.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 10 µg
Lane 2: Mouse kidney lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 3min
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img25422.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
Lane 1: Mouse pancreas lysate at 10 µg
Lane 2: Mouse ovary lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 15s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img83385.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution
All lanes: Mouse skin lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 15s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img163562.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution
All lanes: Human skin lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 30s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img162232.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
All lanes: Human pancreas lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 30s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img150241.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
All lanes: BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 1s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img151480.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/5000 dilution
All lanes: SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa
Exposure time: 5s
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img436368.jpg "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)
Western blot: Anti-ITGB4 antibody [EPR17517] (ab182120) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab182120 was shown to bind specifically to ITGB4. A band was observed at 200 kDa in wild-type A549 cell lysates with no signal observed at this size in ITGB4 knockout cell line. To generate this image, wild-type and ITGB4 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 40 µg
Lane 2: ITGB4 knockout A549 cell lysate at 40 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 200 kDa
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img25422.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img83385.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img163562.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img162232.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img150241.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img151480.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
![Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-4-antibody-epr17517-ab182120--western-blot-img436368.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 4 antibody [EPR17517] (ab182120)")
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