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Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (AB240259), expandable thumbnail
  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (AB240259), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (AB240259), expandable thumbnail
  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (AB240259), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (AB240259), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Alternative names

CD104, Integrin beta-4, GP150, ITGB4

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17517
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240259 is the carrier-free version of Anti-Integrin beta 4 antibody [EPR17517] ab182120.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Associated diseases and disorders

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/5000 dilution

    All lanes: SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 5s

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/1000 dilution

    All lanes: BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Integrin beta 4 with Anti-Integrin beta 4 antibody [EPR17517] ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin beta 4 antibody [EPR17517] ab182120).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/10000 dilution

    All lanes: Human pancreas lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1000 µg

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Integrin beta 4 with Anti-Integrin beta 4 antibody [EPR17517] ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Cell junction staining on mouse pancreatic acinus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin beta 4 antibody [EPR17517] ab182120).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/20000 dilution

    All lanes: Human skin lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with Anti-Integrin beta 4 antibody [EPR17517] ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human colon epithelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Integrin beta 4 antibody [EPR17517] ab182120).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/20000 dilution

    All lanes: Mouse skin lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 15s

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/1000 dilution

    Lane 1: Mouse pancreas lysate at 10 µg

    Lane 2: Mouse ovary lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa

    Observed band size: 210 kDa

    Exposure time: 15s

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259) at 1/1000 dilution

    Lane 1: Mouse spleen lysate at 10 µg

    Lane 2: Mouse kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 202 kDa

    Exposure time: 3min

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using Anti-Integrin beta 4 antibody [EPR17517] ab182120, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (40 μg)
    Lane 2: ITGB4 knockout HAP1 whole cell lysate (40 μg)

    Lanes 1 - 2: Merged signal (red and green). Green - Anti-Integrin beta 4 antibody [EPR17517] ab182120 observed at 240 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-Integrin beta 4 antibody [EPR17517] ab182120 was shown to specifically recognize ITGB4 in wild-type HAP1 cells alond with additional cross-reactive bands. No band was observed when ITGB4 knockout samples were used. Wild-type and ITGB4 knockout samples were subjected to SDS-PAGE. Anti-Integrin beta 4 antibody [EPR17517] ab182120 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    Predicted band size: 202 kDa

  • Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259), expandable thumbnail

    Western blot - Anti-Integrin beta 4 antibody [EPR17517] - BSA and Azide free (ab240259)

    This data was developed using the same antibody clone in a different buffer formulation (abAB182120).

    Western blot: Anti-ITGB4 antibody [EPR17517] (Anti-Integrin beta 4 antibody [EPR17517] ab182120) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-Integrin beta 4 antibody [EPR17517] ab182120 was shown to bind specifically to ITGB4. A band was observed at 200 kDa in wild-type A549 cell lysates with no signal observed at this size in ITGB4 knockout cell line. To generate this image, wild-type and ITGB4 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Integrin beta 4 antibody [EPR17517] (Anti-Integrin beta 4 antibody [EPR17517] ab182120) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 40 µg

    Lane 2: ITGB4 knockout A549 cell lysate at 40 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 200 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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