AB168386

Anti-Integrin beta 4 antibody [EPR8558(2)]

RabMAb
Recombinant
KO Validated
What is this?

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(2 Publications)

Knockout Tested Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Integrin beta 4 with ab168386 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

ab168386 showing +ve staining in Human skin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

ab168386 showing +ve staining in Human normal spleen.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab168386 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Western blot : Anti-ITGB4 antibody [EPR8558(2)] (ab168386) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab168386 was shown to bind specifically to ITGB4. A band was observed at 200 kDa in wild-type A549 cell lysates with no signal observed at this size in ITGB4 knockout cell line. To generate this image, wild-type and ITGB4 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (ab168386) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 40 µg

Lane 2:

ITGB4 knockout A549 cell lysate at 40 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 200 kDa

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Unknown

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

All lanes:

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (ab168386) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

SW480 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HT29 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 202 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8558(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 479:665-677 PubMed37160555

2023

Exosome-transmitted FOSL1 from cancer-associated fibroblasts drives colorectal cancer stemness and chemo-resistance through transcriptionally activating ITGB4.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Lin,Bo Zhu

Molecular biology of the cell 30:838-850 PubMed30865564

2019

Integrin α6β4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdie Wang,James P Hinton,Jaime M C Gard,Joe G N Garcia,Beatrice S Knudsen,Raymond B Nagle,Anne E Cress

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 4 antibody [EPR8558(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Western blot - Anti-Integrin beta 4 antibody [EPR8558(2)] (AB168386)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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