AB133682

Anti-Integrin beta 4 antibody [EPR8559]

Name: Anti-Integrin beta 4 antibody [EPR8559] (ab133682) | Abcam
Brand: Abcam Limited
SKU: AB133682
Price: 260 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(19 Publications)

Rabbit Recombinant Monoclonal Integrin beta 4 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 19 publications.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

ICC/IF

AbReview43206****

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

ab133682 staining Integrin beta 4 in human breast epithelail cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 25°C. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Qingsu Cheng

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

Immunofluorescent analysis of BxPC-3 cells labelling Integrin beta 4 with ab133682 at 1/250 dilution.

OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Integrin beta 4 antibody [EPR8559] - BSA and Azide free
660 APC

APC Anti-Integrin beta 4 antibody [EPR8559]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Integrin beta 4 antibody [EPR8559]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Integrin beta 4 antibody [EPR8559]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Integrin beta 4 antibody [EPR8559]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Integrin beta 4 antibody [EPR8559]
578 PE

PE Anti-Integrin beta 4 antibody [EPR8559]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8559

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

style

left-column

Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Materials (Basel, Switzerland) 18: PubMed40731515

2025

Attachment of Human Epithelial Cells to an Anodized Titanium Surface.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiko Akashi,Hayato Hashiguchi,Yoshitaka Yamaoka,Kei Nakajima,Katsutoshi Kokubun,Yoshiaki Shimoo,Kenichi Matsuzaka

Non-coding RNA 11: PubMed40407591

2025

Bromodomain and Extra-Terminal Family Proteins BRD2, BRD3, and BRD4 Contribute to H19-Dependent Transcriptional Regulation of Cell Adhesion Molecules, Modulating Metastatic Dissemination Program in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Pecci,Melissa Borsa,Aurora Aiello,Sara De Martino,Luca Cis,Cristian Ripoli,Dante Rotili,Francesco Pierconti,Francesco Pinto,Claudio Grassi,Carlo Gaetano,Antonella Farsetti,Simona Nanni

BMC biology 22:262 PubMed39548509

2024

Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyu Zhong,Lu Zheng,Yi Wu,Shujin Sun,Qing Luo,Guanbin Song,Dongyuan Lü,Mian Long

Biomedicines 12: PubMed39457633

2024

Prognostic Impact of H19/Cell Adhesion Molecules Circuitry on Prostate Cancer Biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Pecci,Francesco Pierconti,Angela Carlino,Francesco Pinto,Ugo Gradilone,Sara De Martino,Dante Rotili,Claudio Grassi,Alfredo Pontecorvi,Carlo Gaetano,Lidia Strigari,Antonella Farsetti,Simona Nanni

International journal of biological sciences 20:1045-1063 PubMed38322121

2024

The homeoprotein HOXB2 limits triple-negative breast carcinogenesis via extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Oh,Clara Yuri Kim,Da Som Jeong,Yu Cheon Kim,Myoung Hee Kim,Je-Yoel Cho

Cancer cell international 24:56 PubMed38317193

2024

Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Pecci,Fabiola Troisi,Aurora Aiello,Sara De Martino,Angela Carlino,Vincenzo Fiorentino,Cristian Ripoli,Dante Rotili,Francesco Pierconti,Maurizio Martini,Manuela Porru,Francesco Pinto,Antonello Mai,Pier Francesco Bassi,Claudio Grassi,Carlo Gaetano,Alfredo Pontecorvi,Lidia Strigari,Antonella Farsetti,Simona Nanni

International journal of molecular sciences 24: PubMed37373423

2023

7-Ketocholesterol Promotes Retinal Pigment Epithelium Senescence and Fibrosis of Choroidal Neovascularization via IQGAP1 Phosphorylation-Dependent Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Wang,Aniket Ramshekar,Thaonhi Cung,Chris Wallace-Carrete,Chandler Zaugg,Jasmine Nguyen,Gregory J Stoddard,M Elizabeth Hartnett

The Journal of dermatology 50:999-1007 PubMed37157942

2023

Barrier function and ultrastructure characteristics of epidermis in patients with primary cutaneous amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Zhang,Ya Le,Junyi Guo,Fangfang Wu,Qing Li,Ping Lu

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology 33:e13871 PubMed36282138

2022

ITGB4 deficiency induces DNA damage by downregulating HDAC1 in airway epithelial cells under stress stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Xu,Ye Yao,Huijun Liu,Ming Yang,Lin Yuan,Xizi Du,Yu Yang,Ling Qin,Weijie Wang,Kai Zhou,Xinyu Wu,Chi Liu

BMC cancer 22:558 PubMed35585515

2022

Laminin-integrin a6b4 interaction activates notch signaling to facilitate bladder cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Hao,Daming Yang,Tianpei Liu,Shucheng Liu,Xinsheng Lu,Libo Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 4 antibody [EPR8559]

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

OI-RD Scanning - Anti-Integrin beta 4 antibody [EPR8559] (AB133682)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise