AB29042

Anti-Integrin beta 4 antibody [M126]

Name: Anti-Integrin beta 4 antibody [M126] (ab29042) | Abcam
Brand: Abcam Limited
SKU: AB29042
Price: 470 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(33 Publications)

Mouse Monoclonal Integrin beta 4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGB4.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

2 Images

Flow Cytometry (Intracellular) - Anti-Integrin beta 4 antibody [M126] (AB29042)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin beta 4 antibody [M126] (AB29042)

Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight^® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Western blot - Anti-Integrin beta 4 antibody [M126] (AB29042)

All lanes:

Western blot - Anti-Integrin beta 4 antibody [M126] (ab29042)

Lane 1:

A431 (25 µg/lane) serum starved overnight

Lane 2:

A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min

Predicted band size: 202 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M126

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human ITGB4. The exact immunogen used to generate this antibody is proprietary information.

P16144

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

protein A chromatography.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10729 PubMed39737945

2024

ITGB4/CD104 mediates zika virus attachment and infection.

Applications

Unspecified application

Species

Unspecified reactive species

Haolong Cong,Jiuqiang Wang,Ning Du,Lei Song,Ruigang Wang,Yang Yang,Rong Lei,Tie-Shan Tang,Chang-Mei Liu,Shuifang Zhu,Xiaodong Han

Cell reports 43:114121 PubMed38615320

2024

Context-dependent roles for ubiquitous mitochondrial creatine kinase CKMT1 in breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Vinay Ayyappan,Nicole M Jenkinson,Caitlin M Tressler,Zheqiong Tan,Menglin Cheng,Xinyi Elaine Shen,Alejandro Guerrero,Kanchan Sonkar,Ruoqing Cai,Oluwatobi Adelaja,Sujayita Roy,Alan Meeker,Pedram Argani,Kristine Glunde

Acta biochimica et biophysica Sinica 56:753-762 PubMed38602002

2024

CTNNAL1 promotes the structural integrity of bronchial epithelial cells through the RhoA/ROCK1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Liu,Jinmei Wang,Yurong Tan,Chi Liu,Xiangping Qu,Huijun Liu,Meiling Tan,Changqing Deng,Xiaoqun Qin,Yang Xiang

Proceedings of the National Academy of Sciences of the United States of America 120:e2304288120 PubMed37844244

2023

Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling.

Applications

Unspecified application

Species

Unspecified reactive species

James R W Conway,Aleksi Isomursu,Gautier Follain,Ville Härmä,Eva Jou-Ollé,Nicolas Pasquier,Eetu P O Välimäki,Juha K Rantala,Johanna Ivaska

Nature materials 22:1409-1420 PubMed37709930

2023

The laminin-keratin link shields the nucleus from mechanical deformation and signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zanetta Kechagia,Pablo Sáez,Manuel Gómez-González,Brenda Canales,Srivatsava Viswanadha,Martín Zamarbide,Ion Andreu,Thijs Koorman,Amy E M Beedle,Alberto Elosegui-Artola,Patrick W B Derksen,Xavier Trepat,Marino Arroyo,Pere Roca-Cusachs

Journal of cell science 135: PubMed36468336

2022

Laminin matrix adhesion regulates basal mammary epithelial cell identity.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna I Englund,Hien Bui,Defne D Dinç,Oona Paavolainen,Tomás McKenna,Suvi Laitinen,Pauliina Munne,Juha Klefström,Emilia Peuhu,Pekka Katajisto

Molecular and cellular biology 42:e0055921 PubMed35708320

2022

Aberrant Neuregulin 1/ErbB Signaling in Charcot-Marie-Tooth Type 4D Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ting Jiang,Yu-Hui Chen,Jie-Hong Huang,Wei-Fang Tong,Ling-Jing Jin,Li-Xi Li

Cell adhesion & migration 16:13-24 PubMed35137648

2022

TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masaru Mezawa,Yuto Tsuruya,Arisa Yamaguchi,Mizuho Yamazaki-Takai,Tetsuro Kono,Hiroyuki Okada,Christopher A McCulloch,Yorimasa Ogata

International journal of biological sciences 18:1254-1270 PubMed35173551

2022

Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Ling Tan,Wen-Jie Huang,Yue Wang,Lei Liu,Yan Pan,Jing-Jing Li,Jiang Zhang,Mingxing Ouyang,Xiang-Ping Qu,Hui-Jun Liu,Chi Liu,Dan Zeng,Xiao-Qun Qin,Linhong Deng,Yang Xiang

Experimental eye research 211:108720 PubMed34389315

2021

Characterisation of corneas following different time and storage methods for their use as a source of stem-like limbal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Romo-Valera,Miguel Pérez-Garrastachu,Raquel Hernáez-Moya,Maddalen Rodriguez-Astigarraga,Paula Romano-Ruiz,Jaime Etxebarria,Jon Arluzea,Noelia Andollo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 4 antibody [M126]

Flow Cytometry (Intracellular) - Anti-Integrin beta 4 antibody [M126] (AB29042)

Western blot - Anti-Integrin beta 4 antibody [M126] (AB29042)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (33)

Product promise