Goat Polyclonal Integrin beta 5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ITGB5 aa 750 to C-terminus.
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- Clinical and experimental reproductive medicine 48:132-1412021Detrimental effects of lipopolysaccharide on the attachment and outgrowth of various trophoblastic spheroids on human endometrial epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWontae Kim,Jungwon Choi,Hyejin Yoon,Jaewang Lee,Jin Hyun JunPubMed 34078006
- Cell death & disease 10:5742019GLI1 activation by non-classical pathway integrin αβ/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Dong et. al.PubMed 31366904
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ITGB5 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18084
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes A 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C.
Alternative names
Integrin beta-5, ITGB5
Recommended products
Goat Polyclonal Integrin beta 5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ITGB5 aa 750 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ITGB5 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18084
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin beta 5 also known as integrin ß5 is a protein that plays an important role in cell adhesion and signal transduction. It is a component of the integrin family known to mediate interactions between cells and the extracellular matrix (ECM). This protein with a molecular mass of around 90 kDa is expressed in various tissues predominantly in epithelial cells. Integrin beta 5 partners with the alpha V subunit to form the alpha V beta 5 integrin complex which facilitates specific ligand binding such as to vitronectin.
- Biological function summary
Integrin beta 5 contributes to processes like cell migration proliferation and survival often occurring at cell surfaces. It is a part of the integrin complex which plays an important role in these cellular behaviors. Through its interaction with ECM proteins integrin beta 5 influences key biological activities and assists in maintaining tissue architecture. The activity of this integrin impacts how cells respond to their microenvironment influencing cellular fate decisions that are vital to growth and repair mechanisms.
- Pathways
Several signaling pathways involve integrin beta 5. It actively participates in the MAPK/ERK and PI3K/AKT pathways which are essential for transmitting signals that promote cell proliferation and survival. Additionally this integrin works closely with other proteins such as focal adhesion kinase (FAK) and Src family kinases integrating signals that modulate cellular responses to mechanical and biochemical cues from the ECM.
- Associated diseases and disorders
Integrin beta 5 shows involvement in cancer pathogenesis and fibrotic diseases. In cancer its expression correlates with processes such as tumor cell invasion and metastasis impacting prognosis in various cancers including lung and breast cancer. The protein is related to other integrins and ECM components like integrin alpha V and fibronectin contributing to tumor progression. Additionally in fibrotic diseases the dysregulation of integrin beta 5 influences the remodeling of the ECM linking to proteins such as transforming growth factor-beta (TGF-beta) to facilitate pathological tissue changes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Integrin beta 5 antibody (ab31327)
Primary incubation was for 1 hour. Detected by chemiluminescence
All lanes: Western blot - Anti-Integrin beta 5 antibody (ab31327) at 0.1 µg/mL
All lanes: Tissue lysates prepared from Human Ovary at 35 µg
Predicted band size: 88 kDa
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