AB184312

Anti-Integrin beta 5 antibody [EPR19170]

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal Integrin beta 5 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

Integrin beta-5, ITGB5

2 Images

Supplier Data

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 : 3 minutes; Lane 3 : 1 minute; Lane 4 : 3 minutes.

All lanes:

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Rat lung lysate at 20 µg

Lane 3:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 4:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1, 2 and 3 : 15 seconds; Lane 4 : 1 minute

All lanes:

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (ab184312) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 88 kDa

Observed band size: 90 kDa

false

Carrier free

Anti-Integrin beta 5 antibody [EPR19170] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19170

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 5 also known as integrin ß5 is a protein that plays an important role in cell adhesion and signal transduction. It is a component of the integrin family known to mediate interactions between cells and the extracellular matrix (ECM). This protein with a molecular mass of around 90 kDa is expressed in various tissues predominantly in epithelial cells. Integrin beta 5 partners with the alpha V subunit to form the alpha V beta 5 integrin complex which facilitates specific ligand binding such as to vitronectin.

Biological function summary

Integrin beta 5 contributes to processes like cell migration proliferation and survival often occurring at cell surfaces. It is a part of the integrin complex which plays an important role in these cellular behaviors. Through its interaction with ECM proteins integrin beta 5 influences key biological activities and assists in maintaining tissue architecture. The activity of this integrin impacts how cells respond to their microenvironment influencing cellular fate decisions that are vital to growth and repair mechanisms.

Pathways

Several signaling pathways involve integrin beta 5. It actively participates in the MAPK/ERK and PI3K/AKT pathways which are essential for transmitting signals that promote cell proliferation and survival. Additionally this integrin works closely with other proteins such as focal adhesion kinase (FAK) and Src family kinases integrating signals that modulate cellular responses to mechanical and biochemical cues from the ECM.

Integrin beta 5 shows involvement in cancer pathogenesis and fibrotic diseases. In cancer its expression correlates with processes such as tumor cell invasion and metastasis impacting prognosis in various cancers including lung and breast cancer. The protein is related to other integrins and ECM components like integrin alpha V and fibronectin contributing to tumor progression. Additionally in fibrotic diseases the dysregulation of integrin beta 5 influences the remodeling of the ECM linking to proteins such as transforming growth factor-beta (TGF-beta) to facilitate pathological tissue changes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V/beta-5 (ITGAV : ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for adenovirus type C.

See full target information Integrin beta-5

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:52 PubMed40234961

2025

Colon cancer exosome-associated HSP90B1 initiates pre-metastatic niche formation in the liver by polarizing M1 macrophage into M2 phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

ShuJie Li,Xue Fu,Deng Ning,QiuMeng Liu,JunFang Zhao,Qi Cheng,XiaoPing Chen,Li Jiang

Molecular medicine reports 30: PubMed39155876

2024

Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphate‑activated protein kinase/E‑cadherin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bolor-Erdene Sarankhuu,Hye Jin Jeon,Da-Un Jeong,Seok-Rae Park,Tae-Hyun Kim,Sung Ki Lee,Ae Ra Han,Seong-Lan Yu,Jaeku Kang

Scientific reports 14:14273 PubMed38902362

2024

Differential extracellular vesicle concentration and their biomarker expression of integrin α/β, EpCAM, and glypican-1 in pancreatic cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Reed Jacobson,Sangdeuk Ha,Sakurako Tani,Shrinwanti Ghosh,Yagna P R Jarajapu,Randall E Brand,Jiha Kim,Yongki Choi

Journal of gastroenterology and hepatology 38:1158-1169 PubMed36878870

2023

Lactobacillus rhamnosus GG alleviates colitis caused by chemotherapy via biofilm formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Qiu,Xuedong Han,Xiangcheng Zhang,Lucy A Teng,Mukesh K Sriwastva,Linlin Zhen,Zhi Li,Minmin Liu,Yi Ren,Shui Wang

Frontiers in oncology 12:887068 PubMed36249018

2022

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wen,Si Chen,Xueting Chen,Hui Qiu,Wei Wang,Nie Zhang,Wanming Liu,Tingting Wang,Xin Ding,Longzhen Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2200546 PubMed35901491

2022

Succinylation Inhibits the Enzymatic Hydrolysis of the Extracellular Matrix Protein Fibrillin 1 and Promotes Gastric Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyun Wang,Xiao Shi,Hongcheng Lu,Chen Zhang,Xiang Li,Tiancheng Zhang,Jiajia Shen,Jianfei Wen

Frontiers in cell and developmental biology 9:649862 PubMed34150753

2021

Periostin/Filamin-A: A Candidate Central Regulatory Axis for Valve Fibrogenesis and Matrix Compaction.

Applications

Unspecified application

Species

Unspecified reactive species

Suniti Misra,Shibnath Ghatak,Ricardo A Moreno-Rodriguez,Russell A Norris,Vincent C Hascall,Roger R Markwald

Communications biology 4:161 PubMed33547385

2021

A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor-RPE interface.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Tang,Jimin Han,Sonal Dalvi,Kannan Manian,Lauren Winschel,Stefanie Volland,Celia A Soto,Chad A Galloway,Whitney Spencer,Michael Roll,Caroline Milliner,Vera L Bonilha,Tyler B Johnson,Lisa Latchney,Jill M Weimer,Erika F Augustine,Jonathan W Mink,Vamsi K Gullapalli,Mina Chung,David S Williams,Ruchira Singh

Life sciences 267:118928 PubMed33358902

2020

RNF185-AS1 promotes hepatocellular carcinoma progression through targeting miR-221-5p/integrin β5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Huang,Ke Li,Rongfu Huang,Jianhua Zhu,Jiayao Yang

BMC complementary medicine and therapies 20:36 PubMed32024510

2020

Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Eun Kim,Joo Eun Lee,Young-Hyun Han,Se-In Lee,Do Kyung Kim,Seok-Rae Park,Seong-Lan Yu,Jaeku Kang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 5 antibody [EPR19170]

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise