Skip to main content

Rabbit Recombinant Monoclonal Integrin beta 5 antibody. Suitable for Dot, WB, IHC-P, Flow Cyt, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092), expandable thumbnail
  • Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (AB309092), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-PFlow CytIPICC/IF
Human
Expected
Tested
Tested
Tested
Expected
Not recommended
Mouse
Expected
Tested
Tested
Tested
Tested
Not recommended
Rat
Expected
Tested
Tested
Expected
Expected
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C.

Alternative names

Integrin beta-5, ITGB5

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 5 antibody. Suitable for Dot, WB, IHC-P, Flow Cyt, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26930-21

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Integrin beta 5 also known as integrin ß5 is a protein that plays an important role in cell adhesion and signal transduction. It is a component of the integrin family known to mediate interactions between cells and the extracellular matrix (ECM). This protein with a molecular mass of around 90 kDa is expressed in various tissues predominantly in epithelial cells. Integrin beta 5 partners with the alpha V subunit to form the alpha V beta 5 integrin complex which facilitates specific ligand binding such as to vitronectin.

Biological function summary

Integrin beta 5 contributes to processes like cell migration proliferation and survival often occurring at cell surfaces. It is a part of the integrin complex which plays an important role in these cellular behaviors. Through its interaction with ECM proteins integrin beta 5 influences key biological activities and assists in maintaining tissue architecture. The activity of this integrin impacts how cells respond to their microenvironment influencing cellular fate decisions that are vital to growth and repair mechanisms.

Pathways

Several signaling pathways involve integrin beta 5. It actively participates in the MAPK/ERK and PI3K/AKT pathways which are essential for transmitting signals that promote cell proliferation and survival. Additionally this integrin works closely with other proteins such as focal adhesion kinase (FAK) and Src family kinases integrating signals that modulate cellular responses to mechanical and biochemical cues from the ECM.

Associated diseases and disorders

Integrin beta 5 shows involvement in cancer pathogenesis and fibrotic diseases. In cancer its expression correlates with processes such as tumor cell invasion and metastasis impacting prognosis in various cancers including lung and breast cancer. The protein is related to other integrins and ECM components like integrin alpha V and fibronectin contributing to tumor progression. Additionally in fibrotic diseases the dysregulation of integrin beta 5 influences the remodeling of the ECM linking to proteins such as transforming growth factor-beta (TGF-beta) to facilitate pathological tissue changes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: Lane 1 and 2: 15 seconds, Lane 3-5: 10 seconds, Lane 6 and 7: 103 seconds.

    All lanes: Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092) at 1/1000 dilution

    Lane 1: A549 (human lu carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: A549 transfected with siRNA specifically targeti ITGB5 whole cell lysate at 20 µg

    Lane 3: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: Mouse lung tissue lysate at 20 µg

    Lane 6: Mouse liver tissue lysate at 20 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Integrin beta 5 with ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat kidney. The section was incubated with ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin beta 5 with ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse kidney. The section was incubated with ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Integrin beta 5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab309092 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309092 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: abab309092 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab309092 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092) at 1/30 dilution

    All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 100 kDa

    Exposure time: 32s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling Integrin beta 5 with ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human gastric adenocarcinoma (PMID: 32395495). The section was incubated with ab309092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling Integrin beta 5 with ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse glioblastoma. The section was incubated with ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Flow cytometric analysis of A549 (human lung carcinoma epithelial cell) cells labelling Integrin beta 5 with ab309092 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Integrin beta 5 with ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human kidney (PMID: 25551756). The section was incubated with ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast) cells labelling Integrin beta 5 with ab309092 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

  • Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092), expandable thumbnail

    Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] (ab309092)

    Dot blot analysis of Integrin beta 5 using ab309092 at 1:1000 (0.524 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100000 dilution.

    Exposure time: 180 seconds

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com