Rabbit Recombinant Monoclonal Integrin beta 5 antibody. Carrier free. Suitable for Dot, WB, IHC-P, Flow Cyt, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--western-blot-img409536.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409534.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093)")
![Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunoprecipitation-img409535.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409530.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (AB309093)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | Flow Cyt | IP | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Expected | Not recommended |
| Mouse | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Expected | Tested | Tested | Expected | Expected | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Integrin alpha-V/beta-5 (ITGAV:ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C.
Alternative names
Integrin beta-5, ITGB5
Recommended products
Rabbit Recombinant Monoclonal Integrin beta 5 antibody. Carrier free. Suitable for Dot, WB, IHC-P, Flow Cyt, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26930-21
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin beta 5 also known as integrin ß5 is a protein that plays an important role in cell adhesion and signal transduction. It is a component of the integrin family known to mediate interactions between cells and the extracellular matrix (ECM). This protein with a molecular mass of around 90 kDa is expressed in various tissues predominantly in epithelial cells. Integrin beta 5 partners with the alpha V subunit to form the alpha V beta 5 integrin complex which facilitates specific ligand binding such as to vitronectin.
- Biological function summary
Integrin beta 5 contributes to processes like cell migration proliferation and survival often occurring at cell surfaces. It is a part of the integrin complex which plays an important role in these cellular behaviors. Through its interaction with ECM proteins integrin beta 5 influences key biological activities and assists in maintaining tissue architecture. The activity of this integrin impacts how cells respond to their microenvironment influencing cellular fate decisions that are vital to growth and repair mechanisms.
- Pathways
Several signaling pathways involve integrin beta 5. It actively participates in the MAPK/ERK and PI3K/AKT pathways which are essential for transmitting signals that promote cell proliferation and survival. Additionally this integrin works closely with other proteins such as focal adhesion kinase (FAK) and Src family kinases integrating signals that modulate cellular responses to mechanical and biochemical cues from the ECM.
- Associated diseases and disorders
Integrin beta 5 shows involvement in cancer pathogenesis and fibrotic diseases. In cancer its expression correlates with processes such as tumor cell invasion and metastasis impacting prognosis in various cancers including lung and breast cancer. The protein is related to other integrins and ECM components like integrin alpha V and fibronectin contributing to tumor progression. Additionally in fibrotic diseases the dysregulation of integrin beta 5 influences the remodeling of the ECM linking to proteins such as transforming growth factor-beta (TGF-beta) to facilitate pathological tissue changes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--western-blot-img409536.jpg "Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: Lane 1 and 2: 15 seconds, Lane 3-5: 10 seconds, Lane 6 and 7: 103 seconds.All lanes: Western blot - Anti-Integrin beta 5 antibody [EPR26930-21] (Anti-Integrin beta 5 antibody [EPR26930-21] ab309092) at 1/1000 dilution
Lane 1: A549 (human lu carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: A549 transfected with siRNA specifically targeti ITGB5 whole cell lysate at 20 µg
Lane 3: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: Mouse lung tissue lysate at 20 µg
Lane 6: Mouse liver tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409534.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat kidney.The section was incubated with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse kidney. The section was incubated with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunoprecipitation-img409535.jpg "Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.Integrin beta 5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2: abab309092 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.All lanes: Immunoprecipitation - Anti-Integrin beta 5 antibody [EPR26930-21] (Anti-Integrin beta 5 antibody [EPR26930-21] ab309092) at 1/30 dilution
All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 100 kDa
Exposure time: 32s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409530.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human gastric adenocarcinoma (PMID: 32395495). The section was incubated with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse glioblastoma. The section was incubated with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--flow-cytometry-img409528.jpg "Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of A549 (human lung carcinoma epithelial cell) cells labelling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409531.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/500 (1.048 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human kidney (PMID: 25551756).The section was incubated with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/Dab (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--flow-cytometry-img409529.jpg "Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast) cells labelling Integrin beta 5 with Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.![Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--dot-blot-img409527.jpg "Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)
This data was developed using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092, the same antibody clone in a different buffer formulation.
Dot blot analysis of Integrin beta 5 using Anti-Integrin beta 5 antibody [EPR26930-21] ab309092 at 1:1000 (0.524 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
![Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--flow-cytometry-img409528.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img409531.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
![Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--flow-cytometry-img409529.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
![Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-5-antibody-epr26930-21-bsa-and-azide-free-ab309093--dot-blot-img409527.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Integrin beta 5 antibody [EPR26930-21] - BSA and Azide free (ab309093)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com