AB137058

Anti-Integrin beta 7 antibody [EP5948]

Name: Anti-Integrin beta 7 antibody [EP5948] (ab137058) | Abcam
Brand: Abcam Limited
SKU: AB137058
Price: 380 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Integrin beta 7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Integrin beta-7, Gut homing receptor beta subunit, ITGB7

2 Images

Lab

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] (AB137058)

purifiedab137058 at 1/50 dilution (2μg) immunoprecipitating Integrin beta 7 in IM-9 whole cell lysate.
Lane 1 (input) : IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10μg
Lane 2 (+) : ab137058 + IM-9 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137058 in IM-9 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 100-120 kDa

All lanes:

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] (ab137058)

Predicted band size: 87 kDa

false

Unknown

Western blot - Anti-Integrin beta 7 antibody [EP5948] (AB137058)

All lanes:

Western blot - Anti-Integrin beta 7 antibody [EP5948] (ab137058) at 1/1000 dilution

All lanes:

IM-9 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 87 kDa

false

Carrier free

Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free
Carrier free

Anti-Integrin beta 7 antibody [EP5948] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5948

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 7 also known as beta-7 integrin beta 7 or β7 is a protein encoded by the ITGB7 gene. It forms a heterodimer most commonly with integrin alpha 4 (forming α4β7 integrin) or integrin alpha E (forming αEβ7 integrin). The molecular mass of integrin beta 7 is approximately 84 kDa. It is expressed in the intestinal mucosa particularly associated with immune cells such as T lymphocytes and dendritic cells. Integrin beta 7 plays an important role in cell adhesion and migration by binding to its ligands which are present on endothelial cells in the gut.

Biological function summary

Integrin beta 7 facilitates cell adhesion and signaling processes important for immune surveillance and inflammation regulation. As part of heterodimer complexes like α4β7 and αEβ7 integrins it mediates adhesion of lymphocytes to mucosal tissues. This interaction is necessary for directing lymphocytes to sites of inflammation in the intestinal tract. The α4β7 integrin particularly binds to the addressin MAdCAM-1 which is expressed on high endothelial venules in the gut-associated lymphoid tissues.

Pathways

Integrin beta 7 is involved in both integrin-mediated signaling and leukocyte extravasation pathways. These pathways regulate the localization and migration of immune cells to target tissues which is essential for efficient immune responses. The protein works together with integrin alpha 4 and alpha E components to facilitate these processes. Integrin signaling activates various intracellular cascades including those involving kinases that modulate cell adhesion and migration.

Integrin beta 7 has significant implications in inflammatory bowel disease (IBD) and other immune-mediated conditions like celiac disease. Elevated expression of integrin beta 7 particularly in the α4β7 complex associates with the overactive lymphocyte trafficking found in IBD. As a result therapies targeting this integrin like vedolizumab aim to modulate lymphocyte migration. The involvement of proteins such as MAdCAM-1 in this integrin's function links it to the pathophysiology of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGA4/ITGB7 (alpha-4/beta-7) (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT) (Probable). Integrin ITGA4/ITGB7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract (PubMed : 10837471, PubMed : 14608374). Also interacts with VCAM1 and fibronectin, an extracellular matrix component (Probable). It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin (Probable). Interactions involve the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin (Probable). Integrin ITGAE/ITGB7 (alpha-E/beta-7, HML-1) is a receptor for E-cadherin (PubMed : 10837471).. (Microbial infection) Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120.

See full target information ITGB7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806782

2025

The Loss of Complex I in Renal Oncocytoma Is Associated with Defective Mitophagy Due to Lysosomal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lin,Neal Patel,Lucia Fernandez-Del-Rio,Cristiane Benica,Blake Wilde,Eirini Christodoulou,Shinji Ohtake,Anhyo Jeong,Aboubacar Kaba,Nedas Matulionis,Randy Caliliw,Xiaowu Gai,Heather Christofk,David Shackelford,Brian Shuch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 7 antibody [EP5948]

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] (AB137058)

Western blot - Anti-Integrin beta 7 antibody [EP5948] (AB137058)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise