AB246697

Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Integrin beta 7 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Integrin beta-7, Gut homing receptor beta subunit, ITGB7

2 Images

Lab

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free (AB246697)

This data was developed using ab137058, the same antibody clone in a different buffer formulation.

Purified ab137058 at 1/50 dilution (2µg) immunoprecipitating Integrin beta 7 in IM-9 whole cell lysate.

Lane 1 (input) : IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysate 10µg.

Lane 2 (+) : ab137058 + IM-9 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137058 in IM-9 whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.

Blocking Buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM/TBST.

Observed band size : 100-120 kDa.

All lanes:

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] (<a href='/en-us/products/primary-antibodies/integrin-beta-7-antibody-ep5948-ab137058'>ab137058</a>)

Predicted band size: 87 kDa

false

Unknown

Western blot - Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free (AB246697)

This data was developed using ab137058, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Integrin beta 7 antibody [EP5948] (<a href='/en-us/products/primary-antibodies/integrin-beta-7-antibody-ep5948-ab137058'>ab137058</a>) at 1/1000 dilution

All lanes:

IM-9 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 87 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5948

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab246697 is the carrier-free version of ab137058.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Endotoxin level is less than 1 EU/ml as determined by the TAL test.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 7 also known as beta-7 integrin beta 7 or β7 is a protein encoded by the ITGB7 gene. It forms a heterodimer most commonly with integrin alpha 4 (forming α4β7 integrin) or integrin alpha E (forming αEβ7 integrin). The molecular mass of integrin beta 7 is approximately 84 kDa. It is expressed in the intestinal mucosa particularly associated with immune cells such as T lymphocytes and dendritic cells. Integrin beta 7 plays an important role in cell adhesion and migration by binding to its ligands which are present on endothelial cells in the gut.

Biological function summary

Integrin beta 7 facilitates cell adhesion and signaling processes important for immune surveillance and inflammation regulation. As part of heterodimer complexes like α4β7 and αEβ7 integrins it mediates adhesion of lymphocytes to mucosal tissues. This interaction is necessary for directing lymphocytes to sites of inflammation in the intestinal tract. The α4β7 integrin particularly binds to the addressin MAdCAM-1 which is expressed on high endothelial venules in the gut-associated lymphoid tissues.

Pathways

Integrin beta 7 is involved in both integrin-mediated signaling and leukocyte extravasation pathways. These pathways regulate the localization and migration of immune cells to target tissues which is essential for efficient immune responses. The protein works together with integrin alpha 4 and alpha E components to facilitate these processes. Integrin signaling activates various intracellular cascades including those involving kinases that modulate cell adhesion and migration.

Integrin beta 7 has significant implications in inflammatory bowel disease (IBD) and other immune-mediated conditions like celiac disease. Elevated expression of integrin beta 7 particularly in the α4β7 complex associates with the overactive lymphocyte trafficking found in IBD. As a result therapies targeting this integrin like vedolizumab aim to modulate lymphocyte migration. The involvement of proteins such as MAdCAM-1 in this integrin's function links it to the pathophysiology of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGA4/ITGB7 (alpha-4/beta-7) (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT) (Probable). Integrin ITGA4/ITGB7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract (PubMed : 10837471, PubMed : 14608374). Interacts also with VCAM1 and fibronectin, an extracellular matrix component (Probable). It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin (Probable). Interactions involve the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin (Probable). Integrin ITGAE/ITGB7 (alpha-E/beta-7, HML-1) is a receptor for E-cadherin (PubMed : 10837471).. (Microbial infection) Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120.

See full target information ITGB7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover. Oncology 14:28 PubMed36853387

2023

Estrogen-dependent activation of NCOA3 couples with p300 and NF-κB to mediate antiapoptotic genes in ER-positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Zhiyong Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free

Immunoprecipitation - Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free (AB246697)

Western blot - Anti-Integrin beta 7 antibody [EP5948] - Low endotoxin, Azide free (AB246697)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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