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Rabbit Recombinant Monoclonal Integrin beta 8 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (AB317020), expandable thumbnail
  • Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] (AB317020), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (AB317020), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 8 antibody [EPR27246-85] (AB317020), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IFIHC-PIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Integrin alpha-V:beta-8 (ITGAV:ITGB8) is a receptor for fibronectin (PubMed:1918072). It recognizes the sequence R-G-D in its ligands (PubMed:1918072). Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation on the surface of activated regulatory T-cells (Tregs) (Probable). Required during vasculogenesis (By similarity).

Alternative names

Integrin beta-8, ITGB8

Recommended products

Rabbit Recombinant Monoclonal Integrin beta 8 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27246-85
Purification technique
Affinity purification Protein A
Specificity

Not suitable for mouse ICC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Integrin beta 8 also known as β8 integrin or ITGB8 is a transmembrane protein that functions mechanically as part of the integrin family. It forms heterodimers with the alpha v subunit resulting in the integrin alpha v beta 8 complex. This complex plays a role in cell adhesion and signaling by binding to extracellular matrix proteins like latency-associated peptide TGF-β. Integrin beta 8 is expressed in various tissues notably in the brain lungs and intestinal epithelium. The protein has a mass of approximately 85 kDa.

Biological function summary

Integrin beta 8 participates in the regulation of cellular processes such as growth differentiation and migration. The integrin alpha v beta 8 complex serves as a receptor for latent TGF-β activation an important step in modulating TGF-β signaling. This activation influences processes such as extracellular matrix remodeling and immune response regulation. Integrin beta 8 interacts with actin cytoskeleton regulatory proteins to mediate these cellular changes.

Pathways

Integrin beta 8 influences both TGF-β and Wnt signaling pathways. Its role in TGF-β pathway involves activation of latent TGF-β impacting tissue homeostasis and immune regulation. In connection to Wnt signaling integrin beta 8 modulates cell proliferation and differentiation. The protein shares functional relations with other integrins like integrin beta 6 which also interacts with similar ligands and pathways coordinating cellular response during development and repair processes.

Associated diseases and disorders

Integrin beta 8 associates with conditions like brain tumors and fibrosis. For example its upregulation in glioblastoma multiforme contributes to the invasive properties of tumor cells by modulating TGF-β signaling similar to the role observed with integrin beta 6 in fibrotic diseases. This connection emphasizes the therapeutic potential of targeting integrin beta 8 to mitigate disease progression and improve treatment efficacy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling Integrin beta 8 with ab317020 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020), expandable thumbnail

    Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020)

    Low expression: LNCaP (PMID:25886373), liver.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HaCaT (human skin keratinocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Human cerebellum tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Human liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 7: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 8: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 9: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 10: Human brain tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 95 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LNCaP (human prostate carcinoma epithelial cell, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling Integrin beta 8 with ab317020 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Low expression: LNCaP (PMID:25886373)

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 8 antibody [EPR27246-85] (ab317020)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Integrin beta 8 with ab317020 at 1/50 (10.2 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Low expression: LNCaP (PMID: 25886373).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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