Anti-Integrin beta 8 antibody [EPR27246-85] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 8 antibody [EPR27246-85] - BSA and Azide free (AB317021)

This data was developed using ab317020, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Integrin beta 8 with ab317020 at 1/50 (10.2 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : LNCaP (PMID : 25886373).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] - BSA and Azide free (AB317021)

This data was developed using ab317020, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LNCaP (human prostate carcinoma epithelial cell, Left) / A431 (human epidermoid carcinoma epithelial cell, Right) cells labelling Integrin beta 8 with ab317020 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : LNCaP (PMID : 25886373)

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Integrin beta 8 antibody [EPR27246-85] - BSA and Azide free (AB317021)

This data was developed using ab317020, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling Integrin beta 8 with ab317020 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] - BSA and Azide free (AB317021)

This data was developed using ab317020, the same antibody clone in a different buffer formulation.

Low expression : LNCaP (PMID : 25886373), liver.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Integrin beta 8 antibody [EPR27246-85] (<a href='/en-us/products/primary-antibodies/integrin-beta-8-antibody-epr27246-85-ab317020'>ab317020</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HaCaT (human skin keratinocyte) whole cell lysate at 20 µg

Lane 3:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human cerebellum tissue lysate at 20 µg

Lane 5:

Human liver tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse liver tissue lysate at 20 µg

Lane 8:

Rat brain tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Lane 10:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 95 kDa

false

Exposure time: 180s