Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Images
![Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img103407.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7846.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)")
![Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img38226.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)")
![Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img33902.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135981.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)")
Publications
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e22026332022Transcript Engineered Extracellular Vesicles Alleviate Alloreactive Dynamics in Renal Transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinwen Lin,Junhao Lv,Shiping Yu,Ying Chen,Huiping Wang,Jianghua ChenPubMed 36073846
- The Journal of clinical investigation 132:2022JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJui M Dave,Raja Chakraborty,Aglaia Ntokou,Junichi Saito,Fatima Z Saddouk,Zhonghui Feng,Ashish Misra,George Tellides,Robert K Riemer,Zsolt Urban,Caroline Kinnear,James Ellis,Seema Mital,Robert Mecham,Kathleen A Martin,Daniel M GreifPubMed 34990407
- EJNMMI research 10:1182020Quantitative evaluation of hepatic integrin αβ expression by positron emission tomography imaging using F-FPP-RGD in rats with non-alcoholic steatohepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuichi Hiroyama et. al.PubMed 33026561
- Reproductive biology and endocrinology : RB&E 17:942019Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhangbi Wu et. al.PubMed 31729993
- Frontiers in pharmacology 10:9902019Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCongping Su et. al.PubMed 31619988
- Platelets 31:33-422019The human platelet transcriptome and proteome is altered and pro-thrombotic functional responses are increased during prolonged hypoxia exposure at high altitude.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunxiang Shang et. al.PubMed 30721642
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV:ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed:28302677). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). ITGAV:ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed:29030430). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV:ITGB3 act as a receptor for CD40LG (PubMed:31331973). ITGAV:ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed:10640428). (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Herpes virus 8/HHV-8. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Coxsackievirus A9. (Microbial infection) Acts as a receptor for Hantaan virus. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for Cytomegalovirus/HHV-5. (Microbial infection) Integrin ITGA5:ITGB3 acts as a receptor for Human metapneumovirus. (Microbial infection) Integrin ITGAV:ITGB3 acts aP05556s a receptor for Human parechovirus 1. (Microbial infection) Integrin ITGAV:ITGB3 acts as a receptor for West nile virus. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Alternative names
CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa
Recommended products
Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20825
- Purification technique
- Affinity purification Protein A
- Specificity
The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin beta also known as ITGB is a mechanical transducer involved in cell adhesion and signaling. Integrins are heterodimeric proteins consisting of alpha and beta subunits. The human integrin beta subunit has a molecular mass of about 90-110 kDa and it exists in several isoforms such as ITGB1 ITGB2 ITGB3 among others. These integrin beta subunits are widely expressed across various tissues including epithelial cells endothelial cells and immune cells. They mediate interactions between the extracellular matrix and the cytoskeleton inside the cell influencing cellular responses like movement and survival.
- Biological function summary
Integrin beta participates actively in mediating cell-extracellular matrix (ECM) interactions as part of the integrin complex. By forming dimers with different alpha subunits integrin beta plays an important role in cellular processes such as differentiation proliferation and apoptosis. The ability of integrin beta subunits to interact with different ECM proteins such as fibronectin and collagen allows them to orchestrate tissue-specific functions. Furthermore integrin beta influences signal transduction pathways leading to activation of intracellular signaling molecules like focal adhesion kinase (FAK) and Src family kinases.
- Pathways
Integrin beta significantly impacts cell signaling and communication. It is central to the focal adhesion pathway where it influences the assembly of focal adhesion points that connect cells to the ECM. This connection supports signal cascades that involve Ras-MAPK and PI3K-Akt pathways important for regulating cell proliferation and migration. Integrin beta also interacts with proteins like talin and kindlin enhancing its signaling capabilities and stability at the cell surface thereby affecting cytoskeletal dynamics and cellular motility.
- Associated diseases and disorders
Integrin beta is associated with a range of pathological conditions. Abnormal integrin beta function contributes to cancer progression and metastasis as its altered expression can affect cell adhesion and invasion. Additionally integrin beta dysfunction is implicated in inflammatory diseases like multiple sclerosis where it interacts with proteins such as VCAM-1 to facilitate immune cell infiltration into the central nervous system. Understanding these interactions and their effects on disease development remains a focus for therapeutic interventions targeting integrin beta and its pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img103407.jpg "Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)
Exposure time : Lane 1-3: 3 minutes; Lane 4: 15 seconds; Lane 5: 3 seconds.Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).
All lanes: Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
Lane 4: HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg
Lane 5: Human fetal spleen lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Observed band size: 100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7846.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on platelets of human spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
![Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img38226.jpg "Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)
Comparison of staining with an antibody specific to Integrin beta 3 (Anti-Integrin beta 3 antibody [EPR2342] ab119992).The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.
Exposure:
For ab210515: 1, 2, 5 lanes: 3 s; 3, 4 lanes: 37 s; 6 lane: 3 min; 7 lane: 15 s; 8 lane: 114 s.
For Anti-Integrin beta 3 antibody [EPR2342] ab119992: 1, 2 lane: 10 s; 3~8 lanes: 3 min
All lanes: Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution or Anti-Integrin beta 3 antibody [EPR2342] (Anti-Integrin beta 3 antibody [EPR2342] ab119992) at 1/500
Lane 1: U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 2: HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 3: LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 6: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 7: C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg with 5% NFDM/TBST
Lane 8: Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 110 kDa
![Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--western-blot-img33902.jpg "Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515)
Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds; Lane 3: 3 minutes; Lane 4: 15 seconds.Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 9610392).
All lanes: Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution
Lane 1: Mouse kidney lysate at 10 µg
Lane 2: C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Lane 3: Rat heart lysate at 10 µg
Lane 4: Rat liver lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 87 kDa
Observed band size: 100 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135981.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of mouse spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57216.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of rat spleen (PMID: 22434870). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515), expandable thumbnail](https://content.abcam.com/products/images/integrin-beta-antibody-epr20825-ab210515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57216.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (ab210515)")
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