AB210515

Anti-Integrin beta antibody [EPR20825]

20ul selling size
RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal Integrin beta 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

CD61, GP3A, ITGB3, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on platelets of human spleen (PMID : 22434870). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of rat spleen (PMID : 22434870). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Integrin beta with ab210515 at 1/20000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on megakaryocytes and weak staining on platelets of mouse spleen (PMID : 22434870). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval performed using ab208572 (Universal HIER antigen retrieval reagent).

Lab

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution or Anti-Integrin beta 3 antibody [EPR2342] (ab119992) at 1/500.

Comparison of staining with an antibody specific to Integrin beta 3 (ab119992).

The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins : beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

Exposure :

For ab210515 : 1, 2, 5 lanes : 3 s; 3, 4 lanes : 37 s; 6 lane : 3 min; 7 lane : 15 s; 8 lane : 114 s.

For ab119992 : 1, 2 lane : 10 s; 3~8 lanes : 3 min

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 8:

Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

Lanes 1 - 8:

Western blot - Anti-Integrin beta 3 antibody [EPR2342] (<a href='/en-us/products/primary-antibodies/integrin-beta-3-antibody-epr2342-ab119992'>ab119992</a>) at 1/500 dilution

Lane 1:

U-87MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Lane 3:

LnCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg

Lane 6:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 7:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

Lane 8:

Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 110 kDa

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Supplier Data

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Exposure time : Lane 1-3 : 3 minutes; Lane 4 : 15 seconds; Lane 5 : 3 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9610392).

All lanes:

Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg

Lane 3:

Rat spleen lysate at 20 µg

Lane 4:

HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Lane 5:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 87 kDa

Observed band size: 100 kDa

true

Supplier Data

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Exposure time : Lane 1 : 30 seconds; Lane 2 : 3 seconds; Lane 3 : 3 minutes; Lane 4 : 15 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9610392).

All lanes:

Western blot - Anti-Integrin beta antibody [EPR20825] (ab210515) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 3:

Rat heart lysate at 10 µg

Lane 4:

Rat liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 87 kDa

Observed band size: 100 kDa

true

Carrier free

Anti-Integrin beta antibody [EPR20825] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20825

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The Integrin beta 3 immunogen used for this product shares the following sequence identities with other Integrin beta proteins: beta 5 – 61%, beta 6 – 53%, beta 7 – 48%, beta 1 – 47%, beta 2 – 46%, beta 8 – 42%, beta 4 – 38%. Specific cross-reactivities have not been determined experimentally, but based on our data we expect that the antibody cross-reacts with 1 or more of these proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta also known as ITGB is a mechanical transducer involved in cell adhesion and signaling. Integrins are heterodimeric proteins consisting of alpha and beta subunits. The human integrin beta subunit has a molecular mass of about 90-110 kDa and it exists in several isoforms such as ITGB1 ITGB2 ITGB3 among others. These integrin beta subunits are widely expressed across various tissues including epithelial cells endothelial cells and immune cells. They mediate interactions between the extracellular matrix and the cytoskeleton inside the cell influencing cellular responses like movement and survival.

Biological function summary

Integrin beta participates actively in mediating cell-extracellular matrix (ECM) interactions as part of the integrin complex. By forming dimers with different alpha subunits integrin beta plays an important role in cellular processes such as differentiation proliferation and apoptosis. The ability of integrin beta subunits to interact with different ECM proteins such as fibronectin and collagen allows them to orchestrate tissue-specific functions. Furthermore integrin beta influences signal transduction pathways leading to activation of intracellular signaling molecules like focal adhesion kinase (FAK) and Src family kinases.

Pathways

Integrin beta significantly impacts cell signaling and communication. It is central to the focal adhesion pathway where it influences the assembly of focal adhesion points that connect cells to the ECM. This connection supports signal cascades that involve Ras-MAPK and PI3K-Akt pathways important for regulating cell proliferation and migration. Integrin beta also interacts with proteins like talin and kindlin enhancing its signaling capabilities and stability at the cell surface thereby affecting cytoskeletal dynamics and cellular motility.

Integrin beta is associated with a range of pathological conditions. Abnormal integrin beta function contributes to cancer progression and metastasis as its altered expression can affect cell adhesion and invasion. Additionally integrin beta dysfunction is implicated in inflammatory diseases like multiple sclerosis where it interacts with proteins such as VCAM-1 to facilitate immune cell infiltration into the central nervous system. Understanding these interactions and their effects on disease development remains a focus for therapeutic interventions targeting integrin beta and its pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V/beta-3 (ITGAV : ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B : ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed : 9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). ITGAV : ITGB3 binds to fractalkine (CX3CL1) and acts as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415, PubMed : 24789099). ITGAV : ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGAV : ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed : 18441324). ITGAV : ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling (PubMed : 28302677). ITGAV : ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 19578119). ITGAV : ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464). ITGAV : ITGB3 binds to IL1B and this binding is essential for IL1B signaling (PubMed : 29030430). ITGAV : ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). ITGAV : ITGB3 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 12807887). In brain, plays a role in synaptic transmission and plasticity. Involved in the regulation of the serotonin neurotransmission, is required to localize to specific compartments within the synapse the serotonin receptor SLC6A4 and for an appropriate reuptake of serotonin. Controls excitatory synaptic strength by regulating GRIA2-containing AMPAR endocytosis, which affects AMPAR abundance and composition (By similarity). ITGAV : ITGB3 act as a receptor for CD40LG (PubMed : 31331973). ITGAV : ITGB3 acts as a receptor for IBSP and promotes cell adhesion and migration to IBSP (PubMed : 10640428). Integrin ITGA2B : ITGB3 is also the receptor of the erythrocyte-specific ICAM4 ligand involved in heterotypic cell-cell adhesion between erythrocytes and activated platelets (PubMed : 12477717).. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Herpes virus 8/HHV-8.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Coxsackievirus A9.. (Microbial infection) Acts as a receptor for Hantaan virus.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for Cytomegalovirus/HHV-5.. (Microbial infection) Integrin ITGA5 : ITGB3 acts as a receptor for Human metapneumovirus.. (Microbial infection) Integrin ITGAV : ITGB3 acts aP05556s a receptor for Human parechovirus 1.. (Microbial infection) Integrin ITGAV : ITGB3 acts as a receptor for West nile virus.. (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

See full target information ITGB3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:5790-5800 PubMed40365277

2025

Comparative study of [F]AlF-NOTA-FAPI-RGD and [F]FDG/[F]AlF-NOTA-FAPI-04 PET/CT in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Gao,Zhiwei Ma,Ziyang Zhu,Zhichuan Yang,Bo Chen,Xiaoming Wu,Vivianne Jakobsson,Yujiao Deng,Hao Wang,Wei Zhang,Jingjing Zhang

Reproduction & fertility : PubMed40014397

2025

Repeated implantation failures and infertility in patients are strongly associated with elevated integrin B3 due to endometrial copy number variation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Xia Xu,Sheng-Ni Liu,Xiaoyi Xiang,Yan Lei,Yun-Xiu Li,Xiang-Jing Tang,Jian-Mei Yu,Li-Mei Tao,Ze Wu,Li Li

Bioactive materials 29:1-15 PubMed37456580

2023

A suture-free, shape self-adaptive and bioactive PEG-Lysozyme implant for Corneal stroma defect repair and rapid vision restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhou,Shaohua Zhang,Miao Lei,Yixin Cai,Honglei Wang,Jianguo Sun,Jingyuan Cui,Changsheng Liu,Xue Qu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2202633 PubMed36073846

2022

Transcript Engineered Extracellular Vesicles Alleviate Alloreactive Dynamics in Renal Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwen Lin,Junhao Lv,Shiping Yu,Ying Chen,Huiping Wang,Jianghua Chen

The Journal of clinical investigation 132: PubMed34990407

2022

JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Jui M Dave,Raja Chakraborty,Aglaia Ntokou,Junichi Saito,Fatima Z Saddouk,Zhonghui Feng,Ashish Misra,George Tellides,Robert K Riemer,Zsolt Urban,Caroline Kinnear,James Ellis,Seema Mital,Robert Mecham,Kathleen A Martin,Daniel M Greif

EJNMMI research 10:118 PubMed33026561

2020

Quantitative evaluation of hepatic integrin αβ expression by positron emission tomography imaging using F-FPP-RGD in rats with non-alcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuichi Hiroyama,Takemi Rokugawa,Miwa Ito,Hitoshi Iimori,Ippei Morita,Hiroki Maeda,Kae Fujisawa,Keiko Matsunaga,Eku Shimosegawa,Kohji Abe

Reproductive biology and endocrinology : RB&E 17:94 PubMed31729993

2019

Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangbi Wu,Yaojun Cai,Qin Xia,Tiantian Liu,Hao Yang,Fen Wang,Nan Wang,Zhen Yu,Chunying Yin,Qunan Wang,Defa Zhu

Frontiers in pharmacology 10:990 PubMed31619988

2019

Si-Miao-Yong-An Decoction Protects Against Cardiac Hypertrophy and Dysfunction by Inhibiting Platelet Aggregation and Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Congping Su,Qing Wang,Huimin Zhang,Wenchao Jiao,Hui Luo,Lin Li,Xiangyang Chen,Bin Liu,Xue Yu,Sen Li,Wei Wang,Shuzhen Guo

Platelets 31:33-42 PubMed30721642

2019

The human platelet transcriptome and proteome is altered and pro-thrombotic functional responses are increased during prolonged hypoxia exposure at high altitude.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiang Shang,Tana Wuren,Qing Ga,Zhenzhong Bai,Li Guo,Alicia S Eustes,Kyra N McComas,Matthew T Rondina,Rili Ge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin beta antibody [EPR20825]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta antibody [EPR20825] (AB210515)

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Western blot - Anti-Integrin beta antibody [EPR20825] (AB210515)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise