Rabbit Polyclonal Integrin linked ILK antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Integrin-linked protein kinase.
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Publications
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- The Journal of physiology :2020Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance hemodialysis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFulvia Draicchio et. al.PubMed 32969494
- Molecular medicine reports 20:2267-22752019Acupuncture attenuates renal interstitial fibrosis via the TGF‑β/Smad pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Zuo et. al.PubMed 31322212
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Integrin-linked protein kinase. The exact immunogen used to generate this antibody is proprietary information. Database link Q13418
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Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Tested |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Guinea pig, Cow, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Guinea pig, Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Guinea pig, Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed:17420447, PubMed:20005845, PubMed:30367047, PubMed:32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed:30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed:20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed:16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed:9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed:18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed:17420447).
Alternative names
ILK1, ILK2, ILK1, ILK2, ILK, Scaffold protein ILK, ILK-1, ILK-2, Inactive integrin-linked kinase, p59ILK
Recommended products
Rabbit Polyclonal Integrin linked ILK antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Integrin-linked protein kinase.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Integrin-linked protein kinase. The exact immunogen used to generate this antibody is proprietary information. Database link Q13418
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Integrin-linked kinase (ILK) is a 59 kDa protein involved mechanically in linking the extracellular matrix to the intracellular cytoskeleton. It serves as an important adaptor and scaffold in cell signaling facilitating communication between integrins and other cellular components. Expressed widely in various cell types ILK operates at focal adhesions and plays an important role in regulating cell shape movement and survival. The ILK protein is also referred to as 'integrin-linked protein' in some scientific circles and has significant relevance in cellular mechanics.
- Biological function summary
ILK influences numerous cell processes including adhesion migration and proliferation. It forms a complex with other proteins such as parvin and PINCH known collectively as the ILK complex. This complex regulates actin cytoskeletal dynamics affecting cell-extracellular matrix interactions. ILK also modulates signal transduction pathways linked to cell cycle control making it integral to maintaining normal cellular functions. This protein’s interaction within the cell influences processes like angiogenesis and tissue remodeling showing its biological significance.
- Pathways
ILK participates significantly in the PI3K/Akt and Wnt signaling pathways. It interacts with proteins like Akt and GSK-3β playing an essential role in these pathways. In the PI3K/Akt pathway it mediates cell survival and growth by influencing Akt phosphorylation. Within Wnt signaling ILK’s relationship with GSK-3β contributes to regulating β-catenin affecting gene expression downstream. These interactions place ILK as a vital component in cellular signaling networks coordinating important pathway activities.
- Associated diseases and disorders
ILK’s malfunction or aberrant expression is linked to cancer and cardiovascular disorders. In cancer excessive ILK expression often correlates with tumor progression and metastasis likely involving interactions with proteins like β-catenin. In cardiovascular diseases dysregulated ILK function can contribute to heart failure where it associates with proteins like Akt affecting cardiac cell survival. These connections highlight the importance of ILK in pathology showing its role beyond normal physiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Integrin linked ILK antibody (ab227154)
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Integrin linked ILK Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody (ab227154)
10% SDS-PAGE
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1: WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts at 30 µg
Lane 2: Integrin linked ILK knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 51 kDa
Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)
Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 μg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.
Lane 1: 30 μg HeLa whole cell extract
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: ab227154 IP in HeLa whole cell extracts.
10% SDS-PAGE.
All lanes: Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)
Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (ab227154)
Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.
Western blot - Anti-Integrin linked ILK antibody (ab227154)
10% SDS-PAGE
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extracts at 30 µg
Lane 2: C3D80 whole cell extracts at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell extracts at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody (ab227154)
10% SDS-PAGE
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
All lanes: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody (ab227154)
7.5% SDS PAGE
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution
All lanes: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Western blot - Anti-Integrin linked ILK antibody (ab227154)
10 % SDS-PAGE
All lanes: Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: C3D80 whole cell extract at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
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