AB227154

Anti-Integrin linked ILK antibody

Name: Anti-Integrin linked ILK antibody (ab227154) | Abcam
Brand: Abcam Limited
SKU: AB227154
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Integrin linked ILK antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat, Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ILK.

View Alternative Names

ILK1, ILK2, ILK, Scaffold protein ILK, ILK-1, ILK-2, Inactive integrin-linked kinase, p59ILK

8 Images

Supplier Data

Immunoprecipitation - Anti-Integrin linked ILK antibody (AB227154)

Integrin linked ILK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 4 μg of ab227154. Western blot was performed from the immunoprecipitate using ab227154 at1/500 dilution.

Lane 1 : 30 μg HeLa whole cell extract

Lane 2 : Control with 4 μg of preimmune Rabbit IgG.

Lane 3 : ab227154 IP in HeLa whole cell extracts.

10% SDS-PAGE.

All lanes:

Immunoprecipitation - Anti-Integrin linked ILK antibody (ab227154)

Predicted band size: 51 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (AB227154)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (AB227154)

Paraffin embedded mouse kidney tissue stained for Integrin linked ILK with ab227154 (1/500 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Integrin linked ILK antibody (AB227154)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 51 kDa

true

Supplier Data

Western blot - Anti-Integrin linked ILK antibody (AB227154)

10% SDS-PAGE

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Predicted band size: 51 kDa

true

Supplier Data

Western blot - Anti-Integrin linked ILK antibody (AB227154)

10% SDS-PAGE

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extracts at 30 µg

Lane 2:

C3D80 whole cell extracts at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extracts at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extracts at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extracts at 30 µg

Predicted band size: 51 kDa

true

Lab

Western blot - Anti-Integrin linked ILK antibody (AB227154)

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/500 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Integrin linked ILK Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-Integrin linked ILK antibody (AB227154)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

C3D80 whole cell extract at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Predicted band size: 51 kDa

true

Supplier Data

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Integrin linked ILK antibody (ab227154) at 1/1000 dilution

All lanes:

Zebrafish muscle whole cell lysate at 30 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

IHC-P, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human ILK. The exact immunogen used to generate this antibody is proprietary information.

Q13418

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin-linked kinase (ILK) is a 59 kDa protein involved mechanically in linking the extracellular matrix to the intracellular cytoskeleton. It serves as an important adaptor and scaffold in cell signaling facilitating communication between integrins and other cellular components. Expressed widely in various cell types ILK operates at focal adhesions and plays an important role in regulating cell shape movement and survival. The ILK protein is also referred to as 'integrin-linked protein' in some scientific circles and has significant relevance in cellular mechanics.

Biological function summary

ILK influences numerous cell processes including adhesion migration and proliferation. It forms a complex with other proteins such as parvin and PINCH known collectively as the ILK complex. This complex regulates actin cytoskeletal dynamics affecting cell-extracellular matrix interactions. ILK also modulates signal transduction pathways linked to cell cycle control making it integral to maintaining normal cellular functions. This protein’s interaction within the cell influences processes like angiogenesis and tissue remodeling showing its biological significance.

Pathways

ILK participates significantly in the PI3K/Akt and Wnt signaling pathways. It interacts with proteins like Akt and GSK-3β playing an essential role in these pathways. In the PI3K/Akt pathway it mediates cell survival and growth by influencing Akt phosphorylation. Within Wnt signaling ILK’s relationship with GSK-3β contributes to regulating β-catenin affecting gene expression downstream. These interactions place ILK as a vital component in cellular signaling networks coordinating important pathway activities.

ILK’s malfunction or aberrant expression is linked to cancer and cardiovascular disorders. In cancer excessive ILK expression often correlates with tumor progression and metastasis likely involving interactions with proteins like β-catenin. In cardiovascular diseases dysregulated ILK function can contribute to heart failure where it associates with proteins like Akt affecting cardiac cell survival. These connections highlight the importance of ILK in pathology showing its role beyond normal physiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed : 17420447, PubMed : 20005845, PubMed : 30367047, PubMed : 32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed : 30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed : 20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed : 16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed : 9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed : 18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed : 17420447).

See full target information ILK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of physiology : PubMed32969494

2020

Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance hemodialysis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fulvia Draicchio,Stephan van Vliet,Oana Ancu,Scott A Paluska,Kenneth R Wilund,Monika Mickute,Thozhukat Sathyapalan,Derek Renshaw,Peter Watt,Lykke Sylow,Nicholas A Burd,Richard Mackenzie

Molecular medicine reports 20:2267-2275 PubMed31322212

2019

Acupuncture attenuates renal interstitial fibrosis via the TGF‑β/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zuo,Peidong Huang,Yunwu Jiang,Yi Zhang,Miansheng Zhu

View all publications

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Product promise

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Anti-Integrin linked ILK antibody

Immunoprecipitation - Anti-Integrin linked ILK antibody (AB227154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Western blot - Anti-Integrin linked ILK antibody (AB227154)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise