AB52480

Anti-Integrin linked ILK antibody [EP1593Y]

RabMAb
Recombinant
KO Validated
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(28 Publications)

Rabbit Recombinant Monoclonal Integrin linked ILK antibody. Suitable for IHC-P, IP, WB and reacts with Human, African green monkey, Rat samples. Cited in 28 publications.

View Alternative Names

ILK1, ILK2, ILK, Scaffold protein ILK, ILK-1, ILK-2, Inactive integrin-linked kinase, p59ILK

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling Integrin linked ILK with Purified ab52480 at 1/100 dilution (7.13 μg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Immunohistochemical analysis of paraffin-embedded human heart muscle using unpurified ab52480 at a 1/50 dilution.

Unknown

Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

ab52480 (purified) at 1 : 40 dilution (2μg) immunoprecipitating Integrin linked ILK in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab52480 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52480 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Lab

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Lab

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Integrin linked ILK knockout HAP1 cell lysate (20 μg)
Lane 3 : HEK293 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab52480 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab52480 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. Unpurified ab52480 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

Predicted band size: 51 kDa

false

Unknown

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480)

All lanes:

PC12

Predicted band size: 51 kDa

false

Unknown

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution

All lanes:

COS-1 (African green monkey kidney fibroblast-like) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Lab

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/1000 dilution

All lanes:

COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Unknown

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution

Lane 1:

Rat Brain

Lane 2:

Rat Heart

Lane 3:

Rat Kidney

Lane 4:

Rat Spleen

Predicted band size: 51 kDa

false

Exposure time: 3min

Carrier free

Anti-Integrin linked ILK antibody [EP1593Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1593Y

Isotype

IgG

Carrier free

Reacts with

Rat, Human, African green monkey

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with Integrin linked ILK.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "For unpurified use at 1/30 dilution. ", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000 dilution. " }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000 dilution. " }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/2000 - 1/10000 dilution. " }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin-linked kinase (ILK) is a 59 kDa protein involved mechanically in linking the extracellular matrix to the intracellular cytoskeleton. It serves as an important adaptor and scaffold in cell signaling facilitating communication between integrins and other cellular components. Expressed widely in various cell types ILK operates at focal adhesions and plays an important role in regulating cell shape movement and survival. The ILK protein is also referred to as 'integrin-linked protein' in some scientific circles and has significant relevance in cellular mechanics.

Biological function summary

ILK influences numerous cell processes including adhesion migration and proliferation. It forms a complex with other proteins such as parvin and PINCH known collectively as the ILK complex. This complex regulates actin cytoskeletal dynamics affecting cell-extracellular matrix interactions. ILK also modulates signal transduction pathways linked to cell cycle control making it integral to maintaining normal cellular functions. This protein’s interaction within the cell influences processes like angiogenesis and tissue remodeling showing its biological significance.

Pathways

ILK participates significantly in the PI3K/Akt and Wnt signaling pathways. It interacts with proteins like Akt and GSK-3β playing an essential role in these pathways. In the PI3K/Akt pathway it mediates cell survival and growth by influencing Akt phosphorylation. Within Wnt signaling ILK’s relationship with GSK-3β contributes to regulating β-catenin affecting gene expression downstream. These interactions place ILK as a vital component in cellular signaling networks coordinating important pathway activities.

ILK’s malfunction or aberrant expression is linked to cancer and cardiovascular disorders. In cancer excessive ILK expression often correlates with tumor progression and metastasis likely involving interactions with proteins like β-catenin. In cardiovascular diseases dysregulated ILK function can contribute to heart failure where it associates with proteins like Akt affecting cardiac cell survival. These connections highlight the importance of ILK in pathology showing its role beyond normal physiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed : 17420447, PubMed : 20005845, PubMed : 30367047, PubMed : 32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed : 30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed : 20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed : 16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed : 9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed : 18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed : 17420447).

See full target information ILK

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Iranian journal of biotechnology 23:e4098 PubMed40860053

2025

Eicosapentaenoic Acid Modulates TGF-β1/Smad3/ILK Pathway to Attenuate Renal Fibrosis: A Biotechnological Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Wei,Hao Ding,Haitao Li,Di Yin,Juan Cao

Pulmonary circulation 15:e70094 PubMed40330555

2025

Role of MiR-542-3p/Integrin-Linked Kinase/Myocardin Signaling Axis in Hypoxic Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Linqing Li,Weining Zhou,Qingrong Ji,Xianzhao Zhang,Ni Yang,Kaiyou Song,Shunpeng Hu,Cunfei Liu,Zhihong Ou,Fengwei Zhang,Yuda Wei,Jiantong Hou

Stem cells (Dayton, Ohio) 42:861-873 PubMed39169713

2024

Integrin-linked kinase control dental pulp stem cell senescence via the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Xiping Wang,Sha Tian,Linxi Zhou,Li Wang,Xiaohan Liu,Zihan Yang,Guiqiang Fu,Xingguang Liu,Chen Ding,Duohong Zou

Helicobacter 29:e13109 PubMed38951739

2024

Integrin-Linked Kinase in the Development of Gastric Tumors Induced by Helicobacter pylori: Regulation and Prevention Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Boqing Li,Jing He,Ruiqing Zhang,Sisi Liu,Xiaolin Zhang,Zhiqin Li,Chunlei Ma,Wenke Wang,Yingzi Cui,Ying Zhang

Frontiers in immunology 14:1270194 PubMed38077324

2023

Integrin-linked kinase expression in myeloid cells promotes colon tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Afsar U Ahmed,Saleh Almasabi,Ron Firestein,Bryan R G Williams

Frontiers in endocrinology 13:869519 PubMed35721704

2022

Secreted Protein Acidic and Rich in Cysteine Mediates the Development and Progression of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Luo,Xi Sun,Min Tang,Jiahui Wu,Tianwei Qian,Shimei Chen,Zhiyuan Guan,Yanyun Jiang,Yang Fu,Zhi Zheng

Bioengineered 13:10944-10955 PubMed35477364

2022

GDC-0941 activates integrin linked kinase (ILK) expression to cause resistance to GDC-0941 in breast cancer by the tumor necrosis factor (TNF)-α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Chen,Mingming Cheng,Pengcheng Gao,Xiangzhong Zhang,Ganggang Li,Liting Wang,Long Qin,Hongrui Li

American journal of translational research 14:2051-2062 PubMed35422913

2022

Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yayuan Yu,Wencheng Liu,Xinlu Zhan,Yanying Zhong,Ying Feng,Qing Cao,Buzhen Tan

Aging 14:430-442 PubMed35029589

2022

Circular RNA circ_0000515 adsorbs miR-542-3p to accelerate bladder cancer progression via up-regulating ILK expression.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Peng,Jing Guan,Pengfei Leng,Lijun Peng,Manchao Cao,Yuanfa Feng

Cancer research 82:632-647 PubMed34921014

2021

Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Beetham,Billie G C Griffith,Olga Murina,Alexander E P Loftus,David A Parry,Carolin Temps,Jayne Culley,Morwenna Muir,Asier Unciti-Broceta,Andrew H Sims,Adam Byron,Valerie G Brunton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Integrin linked ILK antibody [EP1593Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Immunoprecipitation - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Western blot - Anti-Integrin linked ILK antibody [EP1593Y] (AB52480)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (28)

Product promise