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AB76468

Anti-Integrin linked ILK antibody [EPR1592]

5

(1 Review)

|

(18 Publications)

Rabbit Recombinant Monoclonal Integrin linked ILK antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 18 publications.

View Alternative Names

ILK1, ILK2, ILK, Scaffold protein ILK, ILK-1, ILK-2, Inactive integrin-linked kinase, p59ILK

6 Images
Western blot - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • WB

Unknown

Western blot - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

Flow Cytometry (Intracellular) - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

Western blot - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)
  • WB

Lab

Western blot - Anti-Integrin linked ILK antibody [EPR1592] (AB76468)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Integrin linked ILK knockout HAP1 cell lysate (20 μg)
Lane 3 : Hek293 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab76468 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab76468 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab76468 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

Predicted band size: 51 kDa

false

  • 578 PE

    PE Anti-Integrin linked ILK antibody [EPR1592]

  • Carrier free

    Anti-Integrin linked ILK antibody [EPR1592] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Integrin linked ILK antibody [EPR1592]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Integrin linked ILK antibody [EPR1592]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Integrin linked ILK antibody [EPR1592]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Integrin linked ILK antibody [EPR1592]

  • 660 APC

    APC Anti-Integrin linked ILK antibody [EPR1592]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1592

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/5000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250</strong></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin-linked kinase (ILK) is a 59 kDa protein involved mechanically in linking the extracellular matrix to the intracellular cytoskeleton. It serves as an important adaptor and scaffold in cell signaling facilitating communication between integrins and other cellular components. Expressed widely in various cell types ILK operates at focal adhesions and plays an important role in regulating cell shape movement and survival. The ILK protein is also referred to as 'integrin-linked protein' in some scientific circles and has significant relevance in cellular mechanics.
Biological function summary

ILK influences numerous cell processes including adhesion migration and proliferation. It forms a complex with other proteins such as parvin and PINCH known collectively as the ILK complex. This complex regulates actin cytoskeletal dynamics affecting cell-extracellular matrix interactions. ILK also modulates signal transduction pathways linked to cell cycle control making it integral to maintaining normal cellular functions. This protein’s interaction within the cell influences processes like angiogenesis and tissue remodeling showing its biological significance.

Pathways

ILK participates significantly in the PI3K/Akt and Wnt signaling pathways. It interacts with proteins like Akt and GSK-3β playing an essential role in these pathways. In the PI3K/Akt pathway it mediates cell survival and growth by influencing Akt phosphorylation. Within Wnt signaling ILK’s relationship with GSK-3β contributes to regulating β-catenin affecting gene expression downstream. These interactions place ILK as a vital component in cellular signaling networks coordinating important pathway activities.

ILK’s malfunction or aberrant expression is linked to cancer and cardiovascular disorders. In cancer excessive ILK expression often correlates with tumor progression and metastasis likely involving interactions with proteins like β-catenin. In cardiovascular diseases dysregulated ILK function can contribute to heart failure where it associates with proteins like Akt affecting cardiac cell survival. These connections highlight the importance of ILK in pathology showing its role beyond normal physiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed : 17420447, PubMed : 20005845, PubMed : 30367047, PubMed : 32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed : 30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed : 20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed : 16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed : 9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed : 18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed : 17420447).
See full target information ILK
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 12:e12334 PubMed37357686

2023

Extracellular vesicle-packaged ILK from mesothelial cells promotes fibroblast activation in peritoneal fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Huang,Yuxiang Sun,Long Peng,Juan Sun,Zixin Sha,Hongchun Lin,Yongjie Li,Canming Li,Huiqun Li,Hongli Shang,Yanxu Chen,Xianrui Dou,Zhaoyong Hu,Zengchun Ye,Hui Peng

PloS one 17:e0265901 PubMed35427373

2022

Silencing LncRNA CASC9 inhibits proliferation and invasion of colorectal cancer cells by MiR-542-3p/ILK.

Applications

Unspecified application

Species

Unspecified reactive species

Heping Zhang,Jingfang Wang,Taoyuan Yu,Jingmin Wang,Jun Lu,Zongyang Yu

Biomedicines 9: PubMed34944624

2021

Unravelling the Role of PAX2 Mutation in Human Focal Segmental Glomerulosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Longaretti,Piera Trionfini,Valerio Brizi,Christodoulos Xinaris,Caterina Mele,Matteo Breno,Elena Romano,Roberta Giampietro,Giuseppe Remuzzi,Ariela Benigni,Susanna Tomasoni

Genetics and molecular biology 44:e20200461 PubMed34591063

2021

Periostin regulates osteogenesis of mesenchymal stem cells from ovariectomized rats through actions on the ILK/Akt/GSK-3β Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Silin Liu,Zuolin Jin,Meng Cao,Dandan Hao,Chunrong Li,Doudou Li,Weiwei Zhou

Oncoimmunology 10:1940674 PubMed34249475

2021

Prognostic value of the PrP-ILK-IDO1 axis in the mesenchymal colorectal cancer subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Ghazi,Delphine Le Corre,Camilla Pilati,Julien Taieb,Thomas Aparicio,Audrey Didelot,Shoukat Dedhar,Claire Mulot,Karine Le Malicot,Fatima Djouadi,Aurélien de Reynies,Jean-Marie Launay,Pierre Laurent-Puig,Sophie Mouillet-Richard

Stem cells international 2021:6633111 PubMed33854551

2021

Integrin-Linked Kinase (ILK) Regulates Urinary Stem Cells Differentiation into Smooth Muscle via NF-B Signal Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Liang Huang,Jun-Hong Deng,Jing-Xuan Xie,Zi-Bin Lin,Hui Jiang,Bin Ouyang,Jian-Ming Liu,Yan-Ni Wei,Zhou-da Cai

JCI insight 5: PubMed32161193

2020

C3a receptor blockade protects podocytes from injury in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Morigi,Luca Perico,Daniela Corna,Monica Locatelli,Paola Cassis,Claudia Elisa Carminati,Silvia Bolognini,Carlamaria Zoja,Giuseppe Remuzzi,Ariela Benigni,Simona Buelli

Biological psychiatry 86:377-387 PubMed31126696

2019

Extracellular Matrix Signaling Through β3 Integrin Mediates Cocaine Cue-Induced Transient Synaptic Plasticity and Relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Constanza Garcia-Keller,Daniela Neuhofer,Ana-Clara Bobadilla,Sade Spencer,Vivian C Chioma,Cara Monforton,Peter W Kalivas

Current biology : CB 29:202-216.e7 PubMed30639111

2019

Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Jacquemet,Aki Stubb,Rafael Saup,Mitro Miihkinen,Elena Kremneva,Hellyeh Hamidi,Johanna Ivaska

Experimental & molecular medicine 50:52 PubMed29700289

2018

RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin.

Applications

WB

Species

Unspecified reactive species

So Ra Choe,Yu Na Kim,Chang Gyo Park,Kyung Hwa Cho,Do Yeun Cho,Hoi Young Lee
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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