AB7373

Anti-Interferon alpha 1 antibody [F18]

Name: Anti-Interferon alpha 1 antibody [F18] (ab7373) | Abcam
Brand: Abcam Limited
SKU: AB7373

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(9 Publications)

Rat Monoclonal Interferon alpha 1 antibody. Suitable for Flow Cyt, FuncS (Neut/Block), AP and reacts with Mouse samples. Cited in 9 publications.

View Alternative Names

Interferon alpha-1, IFN-alpha-1, Ifna1

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

F18

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, FuncS (Neut/Block), AP

applications

Specificity

This monoclonal antibody binds and neutralizes both natural and recombinant mouse alpha Interferon 1. This antibody does not cross-react with Human Interferon, Mouse Interferon beta or Mouse Interferon gamma.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "AP" : {"fullname" : "Affinity Purification", "shortname":"AP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "AP-species-checked": "notRecommended", "AP-species-dilution-info": "", "AP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "AP-species-checked": "guaranteed", "AP-species-dilution-info": "", "AP-species-notes": "" } } }

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Product details

With the availability of monoclonal antibodies directed against alpha Interferon, it is possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These Interferon activities can only be distinguished from one another by use of specific neutralizing antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

0.2 µm filtered antibody solution.

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.

Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.

See full target information Ifna1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 163:411-425.e4 PubMed35487288

2022

Toll-like Receptor 9 Pathway Mediates Schlafen-MDSC Polarization During Helicobacter-induced Gastric Metaplasias.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ding,Jayati Chakrabarti,Sulaiman Sheriff,Qian Li,Hahn Nguyen Thi Hong,Ricky A Sontz,Zoe E Mendoza,Amanda Schreibeis,Michael A Helmrath,Yana Zavros,Juanita L Merchant

The EMBO journal 40:e106065 PubMed33615517

2021

5-Fluorouracil efficacy requires anti-tumor immunity triggered by cancer-cell-intrinsic STING.

Applications

Unspecified application

Species

Unspecified reactive species

Jingru Tian,Dingyao Zhang,Vadim Kurbatov,Qinrong Wang,Yadong Wang,Dorthy Fang,Lizhen Wu,Marcus Bosenberg,Mandar D Muzumdar,Sajid Khan,Qianjin Lu,Qin Yan,Jun Lu

Journal of cellular biochemistry 122:958-968 PubMed31773798

2019

Role of miR-106-mediated mitogen-activated protein kinase signaling pathway in oxidative stress injury and inflammatory infiltration in the liver of the mouse with gestational hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Wang,Xiufang Bao,Limeng Song,Yuying Tian,Ping Sun

PloS one 14:e0215062 PubMed30990863

2019

ER intrabody-mediated inhibition of interferon α secretion by mouse macrophages and dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Konrad Büssow,Philipp Themann,Sabine Luu,Paul Pentrowski,Claudia Harting,Mira Majewski,Veith Vollmer,Mario Köster,Martina Grashoff,Rainer Zawatzky,Joop Van den Heuvel,Andrea Kröger,Thomas Böldicke

The Journal of clinical investigation 128:4821-4831 PubMed30277474

2018

E-cadherin expression on multiple myeloma cells activates tumor-promoting properties in plasmacytoid DCs.

Applications

Unspecified application

Species

Unspecified reactive species

Enguang Bi,Rong Li,Laura C Bover,Haiyan Li,Pan Su,Xingzhe Ma,Chunjian Huang,Qiang Wang,Lintao Liu,Maojie Yang,Zhijuan Lin,Jianfei Qian,Weijun Fu,Yong-Jun Liu,Qing Yi

European journal of immunology 45:3158-73 PubMed26350442

2015

Extracellular RNA facilitates hypoxia-induced leukocyte adhesion and infiltration in the lung through TLR3-IFN-γ-STAT1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Indranil Biswas,Bandana Singh,Manish Sharma,Paban K Agrawala,Gausal A Khan

Hepatology (Baltimore, Md.) 60:267-77 PubMed24493010

2014

Plasmacytoid dendritic cell-derived IFN-α promotes murine liver ischemia/reperfusion injury by induction of hepatocyte IRF-1.

Applications

Unspecified application

Species

Unspecified reactive species

Antonino Castellaneta,Osamu Yoshida,Shoko Kimura,Shinichiro Yokota,David A Geller,Noriko Murase,Angus W Thomson

Journal of immunology (Baltimore, Md. : 1950) 186:1477-85 PubMed21172865

2010

Plasmacytoid dendritic cell dichotomy: identification of IFN-α producing cells as a phenotypically and functionally distinct subset.

Applications

Flow Cyt

Species

Mouse

Pia Björck,H Xianne Leong,Edgar G Engleman

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 26:739-47 PubMed17032168

2006

A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression.

Applications

Unspecified application

Species

Unspecified reactive species

Vishwas D Joshi,Dhananjaya V Kalvakolanu,Wilbur Chen,Lei Zhang,Tae Jin Kang,Karen E Thomas,Stefanie N Vogel,Alan S Cross

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Interferon alpha 1 antibody [F18]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise