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Rabbit Recombinant Monoclonal Interferon alpha 2 antibody. Suitable for ICC, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] (AB196221), expandable thumbnail
  • Western blot - Anti-Interferon alpha 2 antibody [EPR19074] (AB196221), expandable thumbnail
  • Immunocytochemistry - Anti-Interferon alpha 2 antibody [EPR19074] (AB196221), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Interferon alpha 2 antibody [EPR19074] (AB196221), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody [EPR19074] (AB196221), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/600

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Produced by macrophages, IFN-alpha have antiviral activities.

Alternative names

Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2C, IFNA2B, IFNA2A, IFNA2

Recommended products

Rabbit Recombinant Monoclonal Interferon alpha 2 antibody. Suitable for ICC, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR19074

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Interferon alpha 2 also known as IFN alpha 2 or IFN alpha 2a/b is a type of cytokine that plays a critical role in the immune response. It is a smaller protein weighing approximately 19.5 kDa mainly expressed by leukocytes such as macrophages and dendritic cells. This protein exhibits antiviral antiproliferative and immunomodulatory activities by binding to specific receptors on target cells. Researchers often study interferon alpha through recombinant protein studies which can be simulated by companies like PeproTech. Additionally ELISA assays facilitate the quantitative measurement of interferon alpha levels in various biological samples.

Biological function summary

Interferon alpha 2 engages in complex signaling with other immune factors to establish an antiviral state in cells. This protein binds to the IFNAR1 and IFNAR2 receptor complex initiating a cascade that activates genes responsible for immune defense. By doing so it triggers the transcription of various antiviral proteins and enhances the activity of immune cells including natural killer cells and macrophages. Its function is also evaluated in reconstitution studies such as PeproTech's IL-2 reconstitution to investigate broader immune functions.

Pathways

Researchers observe interferon alpha 2 predominantly within the JAK-STAT signaling pathway. This pathway is vital in transmitting the extracellular signal through to the gene transcription process leading to an appropriate immune response. STAT proteins such as STAT1 and STAT2 form a transcription complex following activation by JAK kinases. Interferon alpha 2's interactions in the pathway exemplify how this cytokine links with other components for immune regulation coordinating with other proteins like ISGF3 to amplify antiviral responses.

Associated diseases and disorders

Scientists associate interferon alpha 2 with chronic viral infections and certain cancers. For example its therapeutic application has been prominent in treating hepatitis C and some hematological malignancies. In viral infections interferon alpha inhibits viral replication and enhances antiviral immunity. In cancer it suppresses cell proliferation and regulates immune surveillance. The protein's levels and activity can correlate with pathological states influenced by other proteins such as IFN-lambda and TNF-alpha highlighting its role in disease mechanisms and therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221), expandable thumbnail

    Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Interferon alpha 2 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate using ab196221 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab196221 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate 10 μg (Input).

    Lane 2: ab196221 IP in HEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab196221 inHEK-293T (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    The molecular mass observed is due to the presence of the GFP epitope tag on full length IFN alpha 2.

    All lanes: Immunoprecipitation - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Developed using the ECL technique.

    Predicted band size: 22 kDa

    Observed band size: 50 kDa

  • Western blot - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221), expandable thumbnail

    Western blot - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is due to the presence of the GFP epitope tag on full length IFN alpha 2.

    All lanes: Western blot - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221) at 1/5000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector, containing a GFP-Myc-tag, whole cell lysate at 10 µg

    Lane 2: HEK-293T transfected with Interferon alpha 2 expression vector containing a GFP-Myc-tag, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 22 kDa

    Observed band size: 50 kDa

    Exposure time: 3s

  • Immunocytochemistry - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221), expandable thumbnail

    Immunocytochemistry - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Immunocytochemical analysis of agarose-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc)) cells labeling Interferon alpha 2 with ab196221 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use secondary antibody. Positive staining on HEK-293T cells transfected with Interferon alpha 2 (pcDNA3.1 (+)-EGFP-Myc) (image A); and no staining on HEK-293T cells without transfection (image B). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Flow Cytometry (Intracellular) - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Interferon alpha 2 expression vector containing a GFP-His-tag cell line) cells labeling Interferon alpha 2 with ab196221 at 1/600 (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079), at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with either GFP-tagged Interferon alpha 2 expression vector or empty GFP expression vector) cells labeling Interferon alpha 2 with ab196221 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged Interferon alpha 2 expression vector.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of ab196221, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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