AB9386

Anti-Interferon alpha 2b antibody [9D3]

Name: Anti-Interferon alpha 2b antibody [9D3] (ab9386) | Abcam
Brand: Abcam Limited
SKU: AB9386

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(11 Publications )

Mouse Monoclonal Interferon alpha 2 antibody. Suitable for sELISA, FuncS (Neut/Block) and reacts with Recombinant full length protein - Human, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFNA2.

2 Images

Sandwich ELISA - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

sELISA

Unknown

Sandwich ELISA - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

Standard curve for the determination of human interferon-alpha 2b by two-epitope ELISA : capture antibody 9D3 (ab9386), peroxidase-labeled antibody 4E10 (ab9388).

Functional Studies (Neut/Block) - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

FuncS (Neut/Block)

Unknown

Functional Studies (Neut/Block) - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

Neutralizing activity of monoclonal antibody 9D3 in vitro. Interferon antiviral bioassay : L41 cells, mouse encephalomyacarditis virus (EMCV). Antibodies at 5 μg/ml. Control 1 - without EMCV (non-infected cells), control 2 - without interferon, control 3 - irrelevant antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9D3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

sELISA, FuncS (Neut/Block)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IFNA2.

P01563

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "FuncSNeutBlock-species-checked": "testedAndGuaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>This antibody can be used in a sandwich ELISA for the determination of human IFN-a2b. It recognizes different epitopes than anti-Interferon alpha 2b antibody [4E10] (HRP) (<a href='/en-us/products/primary-antibodies/hrp-interferon-alpha-2b-antibody-4e10-ab5258'>ab5258</a>). We recommend using this antibody as a capture antibody and <a href='/en-us/products/primary-antibodies/hrp-interferon-alpha-2b-antibody-4e10-ab5258'>ab5258</a> as a detection antibody.</p>", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR314306-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 2b also known as IFN-alpha 2b or alpha 2b interferon is a type I interferon protein with an average molecular weight of approximately 19000 Daltons. The protein plays a role in the immune response by exhibiting antiviral antiproliferative and immunomodulatory activities. It is expressed mainly in leukocytes such as lymphocytes macrophages and dendritic cells. Researchers can buy interferon online for laboratory uses or create custom alpha 2b interferon for specific research needs.

Biological function summary

Interferon alpha 2b stimulates the immune system to attack pathogens by enhancing antigen presentation and activating natural killer cells and macrophages. It does not function as part of a complex and it acts independently to initiate an antiviral state in cells and increase the host's defense mechanisms. Its role in regulating cell growth and differentiation adds to its importance in maintaining immune homeostasis.

Pathways

Interferon alpha 2b is involved in the JAK-STAT signaling pathway which induces transcription of interferon-stimulated genes that orchestrate an immune response. Additionally it plays a role in the interferon signaling pathway which contributes to innate immunity. Proteins related to it through these pathways include STAT1 and STAT2 which collaborate with IFN alpha-b protein to transmit signals from extracellular stimuli to the nucleus resulting in gene activation.

Interferon alpha 2b is associated with chronic hepatitis C and certain types of cancer including melanoma. It engages with viral proteins in hepatitis C aiming to clear the virus and reduce liver damage. In cancer the antiproliferative properties of interferon alpha 2b help to inhibit tumor cell growth. In these contexts it connects with proteins such as PKR which helps mediate antiviral and anti-tumor responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produced by macrophages, IFN-alpha have antiviral activities.

See full target information IFNA2

Alternative Names

IFNA2A, IFNA2B, IFNA2C, IFNA2, Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Applied microbiology and biotechnology 108:229 PubMed38393430

2024

Construction of viral-based expression vectors for high-level production of human interferon alpha 2b in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Yana Sindarovska,Mykola Kuchuk

Journal of pharmaceutical analysis 12:308-316 PubMed35582401

2022

Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Qin,Maoqin Duan,Dening Pei,Jian Lin,Lan Wang,Peng Zhou,Wenrong Yao,Ying Guo,Xiang Li,Lei Tao,Youxue Ding,Lan Liu,Yong Zhou,Chuncui Jia,Chunming Rao,Junzhi Wang

World journal of stem cells 13:91-114 PubMed33584982

2021

Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jelena G Najdanović,Vladimir J Cvetković,Sanja T Stojanović,Marija Đ Vukelić-Nikolić,Jelena M Živković,Stevo J Najman

Microbial cell factories 15:104 PubMed27287473

2016

Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Landowski,Eero Mustalahti,Ramon Wahl,Laurence Croute,Dhinakaran Sivasiddarthan,Ann Westerholm-Parvinen,Benjamin Sommer,Christian Ostermeier,Bernhard Helk,Juhani Saarinen,Markku Saloheimo

Drug development and industrial pharmacy 42:890-6 PubMed26467418

2015

Dissolving microneedle-based intradermal delivery of interferon-α-2b.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmin Chen,Yuqin Qiu,Suohui Zhang,Yunhua Gao

PloS one 10:e0134723 PubMed26309247

2015

Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Landowski,Anne Huuskonen,Ramon Wahl,Ann Westerholm-Parvinen,Anne Kanerva,Anna-Liisa Hänninen,Noora Salovuori,Merja Penttilä,Jari Natunen,Christian Ostermeier,Bernhard Helk,Juhani Saarinen,Markku Saloheimo

Bioconjugate chemistry 26:452-9 PubMed25629733

2015

Conjugation of PolyPEG to interferon alpha extends serum half-life while maintaining low viscosity of the conjugate.

Applications

Unspecified application

Species

Unspecified reactive species

B Podobnik,B Helk,V Smilović,Š Škrajnar,K Fidler,S Jevševar,A Godwin,P Williams

Biotechnology letters 32:1271-7 PubMed20431909

2010

Improvement of human interferon alpha secretion by Lactococcus lactis.

Applications

Species

Unspecified reactive species

Qiuxiang Zhang,Jin Zhong,Xiaobo Liang,Wenjun Liu,Liandong Huan

Microbiological research 166:111-20 PubMed20227266

2010

Expression of hepatitis B virus surface antigen determinants in Lactococcus lactis for oral vaccination.

Applications

Species

Unspecified reactive species

Qiuxiang Zhang,Jin Zhong,Liandong Huan

Blood 112:2657-66 PubMed18606876

2008

Interferon autoantibodies associated with AIRE deficiency decrease the expression of IFN-stimulated genes.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Kisand,Maire Link,Anette S B Wolff,Anthony Meager,Liina Tserel,Tõnis Org,Astrid Murumägi,Raivo Uibo,Nick Willcox,Katarina Trebusak Podkrajsek,Tadej Battelino,Anna Lobell,Olle Kämpe,Kari Lima,Antonella Meloni,Berrin Ergun-Longmire,Noel K Maclaren,Jaakko Perheentupa,Kai J E Krohn,Hamish S Scott,Eystein S Husebye,Pärt Peterson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Interferon alpha 2b antibody [9D3]

Sandwich ELISA - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

Functional Studies (Neut/Block) - Anti-Interferon alpha 2b antibody [9D3] (AB9386)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (11)

Product promise