Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (AB251131)

This data was developed using ab192264, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Interferon alpha 6 with ab192264 at 1/50 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).

The negative controls are as follows;
1. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
2. Anti-Interferon alpha 6 at 1/50 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (AB251131)

This data was developed using ab192264, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Jurkat cells labelling Interferon alpha 6 (red) with purified ab192264 at dilution of 1/600. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

• WB

Supplier Data

Western blot - Anti-Interferon alpha 6 antibody [EPR16355] - BSA and Azide free (AB251131)

This data was developed using ab192264, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Interferon alpha 6 antibody [EPR16355] (<a href='/en-us/products/primary-antibodies/interferon-alpha-6-antibody-epr16355-ab192264'>ab192264</a>) at 1/5000 dilution

Lane 1:

U937 (Human histiocytic lymphoma cell line) cell lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Lane 3:

Human fetal lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

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