Rabbit Polyclonal Interferon beta antibody. C-terminal. Suitable for WB, sELISA, IHC-P, Neut and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IFNB1 aa 1 to C-terminus.
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- ACS applied materials & interfaces 13:39030-390412021Controlled Organization of Inorganic Materials Using Biological Molecules for Activating Therapeutic Functionalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorgan Chandler,Brian Minevich,Brandon Roark,Mathias Viard,M Brittany Johnson,Mehedi H Rizvi,Thomas A Deaton,Seraphim Kozlov,Martin Panigaj,Joseph B Tracy,Yaroslava G Yingling,Oleg Gang,Kirill A AfoninPubMed 34402305
- Nucleic acids research 48:11785-117982020The immunorecognition, subcellular compartmentalization, and physicochemical properties of nucleic acid nanoparticles can be controlled by composition modification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMorgan Brittany Johnson,Justin R Halman,Daniel K Miller,Joseph S Cooper,Emil F Khisamutdinov,Ian Marriott,Kirill A AfoninPubMed 33091133
- Journal of neuroinflammation 17:1392020Retinoic acid inducible gene-I mediated detection of bacterial nucleic acids in human microglial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Brittany Johnson,Justin R Halman,Amanda R Burmeister,Saralynn Currin,Emil F Khisamutdinov,Kirill A Afonin,Ian MarriottPubMed 32357908
- Scientific reports 9:88272019Combating viral contaminants in CHO cells by engineering innate immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAustin W T Chiang et. al.PubMed 31222165
- Nucleic acid therapeutics 28:200-2082018Modulation of mRNA Translation and Cell Viability by Influenza A Virus Derived Nonstructural Protein 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Liu,Zhen Hua Chia,Johannes Nathaniel Min Hui Liew,Shi Min Or,Kyle K L PhuaPubMed 29634401
- Biomolecules 7:2017Suppression of mRNA Nanoparticle Transfection in Human Fibroblasts by Selected Interferon Inhibiting Small Molecule Compounds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Liu,Manoj N Krishnan,Kyle K L PhuaPubMed 28758979
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IFNB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01574
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Reactivity data
Select an application| WB | sELISA | IHC-P | Neut | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-0.2 µg/mL | Notes - |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Can be used as a capture antibody, with ab84258 as a detection antibody |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes At the recommended concentration ab186669 yields one-half maximal inhibition [ND50] of the biological activity of Human Interferon beta (5 ug/ml). |
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Target data
Function
Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed:10049744, PubMed:10556041, PubMed:6157094, PubMed:6171735, PubMed:7665574, PubMed:8027027, PubMed:8969169). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (PubMed:10049744, PubMed:10556041, PubMed:7665574, PubMed:8027027, PubMed:8969169). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (By similarity). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor (By similarity). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons: acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (By similarity). IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity).
Alternative names
IFB, IFNB, IFNB1, Interferon beta, IFN-beta, Fibroblast interferon
Recommended products
Rabbit Polyclonal Interferon beta antibody. C-terminal. Suitable for WB, sELISA, IHC-P, Neut and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IFNB1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IFNB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01574
- Purification technique
- Affinity purification Immunogen
- Reconstitution
- Reconstitute in water
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Although some customers have been successful in IHC we no longer batch test in this application.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon beta also known as IFN-beta or beta interferon is a type I interferon with a mass of approximately 20 kDa. It originates primarily from fibroblasts and plays an essential role in the immune response. This protein targets and binds to specific cell surface receptors initiating various antiviral states within cells. This action assists in slowing down viral replication and spread. IFN-beta's expression increases in response to viral infections making it an important component in the body's first line of defense against pathogens.
- Biological function summary
Interferon beta functions as an important mediator of immune functions regulating the activity of natural killer cells and macrophages. It also boosts antigen presentation to T cells. Interferon beta belongs to the larger family of interferons which includes IFN-alpha and IFN-gamma each having distinct effects but working together to mount an effective immune response. Though it does not form part of a complex it plays a critical standalone role in immune signaling pathways.
- Pathways
Interferon beta associates strongly with the JAK-STAT signaling pathway. Upon activation it interacts with receptors to phosphorylate STAT proteins mainly STAT1 and STAT2 which then dimerize and translocate to the nucleus to trigger gene expression. Another important pathway is the antiviral response where interferon beta modulates the expression of hundreds of interferon-stimulated genes. These pathways intricately involve other proteins like IFN-alpha and IFN-gamma which work synergistically to establish an effective antiviral environment.
- Associated diseases and disorders
Interferon beta shows significant implications in multiple sclerosis and hepatitis C infection. It is widely used as a therapeutic agent in managing multiple sclerosis by modulating the immune response to reduce inflammation and slow disease progression. Similarly its role in antiviral defense makes it relevant to hepatitis C infection management. In these contexts IFN-alpha and IFN-gamma are also important as they share similar antiviral and immunomodulatory functions that can complement IFN-beta's actions enhancing the treatment's efficacy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Interferon beta antibody - C-terminal (ab186669)
ab186669 was used for Western blot at a concentration of 0.1-0.2 μg/ml, and the detection limit for recombinant Human Interferon beta is 1.5-3.0 ng/lane when used in conjunction with compatible secondary reagents.
All lanes: Western blot - Anti-Interferon beta antibody - C-terminal (ab186669)
Performed under non-reducing conditions.
Predicted band size: 22 kDa
Western blot - Anti-Interferon beta antibody - C-terminal (ab186669)
ab186669 was used for Western blot at a concentration of 0.1-0.2 μg/ml, and the detection limit for recombinant Human Interferon beta is 1.5-3.0 ng/lane when used in conjunction with compatible secondary reagents.
All lanes: Western blot - Anti-Interferon beta antibody - C-terminal (ab186669)
Performed under reducing conditions.
Predicted band size: 22 kDa
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