Rabbit Polyclonal Interferon gamma antibody. Suitable for WB and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ifng aa 50-150 conjugated to Keyhole Limpet Haemocyanin.
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- EJNMMI research 13:1032023[F]FDG PET-MR characterization of aortitis in the IL1rn mouse model of giant-cell arteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Deshayes,Caroline Baugé,Pierre-Antoine Dupont,Christophe Simard,Hanan Rida,Hubert de Boysson,Alain Manrique,Achille AoubaPubMed 38019303
- Science robotics 8:eadd93692023Anti-inflammatory therapy enables robot-actuated regeneration of aged muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS L McNamara,B R Seo,B R Freedman,E B Roloson,J T Alvarez,C T O'Neill,H H Vandenburgh,C J Walsh,D J MooneyPubMed 36947599
- Evidence-based complementary and alternative medicine : eCAM 2022:79718012022Acupuncture Inhibits Morphine Induced-Immune Suppress via Antioxidant System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Jie Zhao,Dae Geon Lee,Chan Sik Park,Chae Ha Yang,Hee Young Kim,Mi Young Lee,Chang-Hyun Song,Il Je Cho,Sang Chan Kim,Sae Kwang Ku,Bong Hyo LeePubMed 36317105
- Haematologica 107:2218-22252022Deregulated JAK3 mediates growth advantage and hemophagocytosis in extranodal nasal-type natural killer/T-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrien Picod,Suella Martino,Pascale Cervera,Gregory Manuceau,Marc Arca,Monica Wittner,YanYan Zhang,He Liang,Florian Beghi,Eric Solary,Fawzia Louache,Paul CoppoPubMed 35511669
- Frontiers in immunology 13:7854222022Intratumor Microbiome Analysis Identifies Positive Association Between and Survival of Chinese Patients With Pancreatic Ductal Adenocarcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Huang,Ning Zhu,Xing Zheng,Yanhong Liu,Haopeng Lu,Xiaochen Yin,Huaijie Hao,Yan Tan,Dongjie Wang,Han Hu,Yong Liang,Xinxing Li,Zhiqian Hu,Yiming YinPubMed 35145519
- Antioxidants (Basel, Switzerland) 10:2021Immunomodulation and Antioxidant Activities as Possible Trypanocidal and Cardioprotective Mechanisms of Major Terpenes from Essential Oils in an Experimental Model of Chronic Chagas Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenerieth Ximena Espinel-Mesa,Clara Isabel González Rugeles,Julio César Mantilla Hernández,Elena E Stashenko,Carlos Andrés Villegas-Lanau,John Jaime Quimbaya Ramírez,Liliana Torcoroma García SánchezPubMed 34829722
- Drug delivery 28:1759-17682021Immunosuppressive effect of PLGA-FK506-NPs in treatment of acute cardiac rejection via topical subcutaneous injection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Deng,Qiaofeng Jin,Ya Wu,Huiling Li,Luyang Yi,Yihan Chen,Tang Gao,Wenyuan Wang,Jing Wang,Qing Lv,Yali Yang,Jia Xu,Wenpei Fu,Li Zhang,Mingxing XiePubMed 34463172
- Virulence 12:360-3762021Vagus nerve plays a pivotal role in CD4+ T cell differentiation during CVB3-induced murine acute myocarditis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Yue-Chun,Xiao-Hong Gu,Ge Li-Sha,De-Pu Zhou,Chao Xing,Xiao-Ling Guo,Lu-Lu Pan,Shi-Yang Song,Li-Li Yu,Guang-Yi Chen,Jia-Feng Lin,Mao-Ping ChuPubMed 33380272
- Biomaterials science 7:3729-37402019Improving acute cardiac transplantation rejection therapy using ultrasound-targeted FK506-loaded microbubbles in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu et. al.PubMed 31403142
- Journal of virology 92:2017Rotavirus Degrades Multiple Interferon (IFN) Type Receptors To Inhibit IFN Signaling and Protects against Mortality from Endotoxin in Suckling Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrish Sen et. al.PubMed 29070687
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Ifng aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P01580
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
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2 products for Alternative Product
Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:11585387, PubMed:8456301). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (By similarity). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (PubMed:20535209, PubMed:25078851).
Alternative names
Interferon gamma, IFN-gamma, Ifng
Recommended products
Rabbit Polyclonal Interferon gamma antibody. Suitable for WB and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ifng aa 50-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Ifng aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P01580
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Interferon gamma antibody (ab216642)
All lanes: Western blot - Anti-Interferon gamma antibody (ab216642) at 1/300 dilution
Lane 1: Diseased mouse lung lysate
Lane 2: Diseased mouse spleen lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 19 kDa
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