Rabbit Polyclonal Interferon gamma antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 104 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFNG.
Images
Publications
Filter by
- Nature communications 14:72352023Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiren Wang,Wenpan Li,Yanhao Jiang,Tuyen Ba Tran,Leyla Estrella Cordova,Jinha Chung,Minhyeok Kim,Georg Wondrak,Jennifer Erdrich,Jianqin LuPubMed 37945606
- Molecular therapy oncolytics 30:86-1022023Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaijin Xia,Keming Lin,Xuemei Wang,FeiLi Chen,Mo Zhou,Yuzhuang Li,Yingtong Lin,Yidan Qiao,Rong Li,Wanying Zhang,Xin He,Fan Zou,Linghua Li,Lijuan Lu,Cancan Chen,WenYu Li,Hui Zhang,Bingfeng LiuPubMed 37593111
- Oncotarget 14:688-6992023Transformation of immunosuppressive mtKRAS tumors into immunostimulatory tumors by Nerofe and Doxorubicin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoel Ohana,Uziel Sandler,Orly Devary,Yoram DevaryPubMed 37395796
- Journal of clinical and experimental hematopathology : JCEH 63:83-892023Role of TGF-beta1 and TNF-alpha1 produced by neoplastic cells in the pathogenesis of fibrosis in patients with hematologic neoplasms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaruko Takizawa,Yoshihiko Araki,Maki Fujishiro,Shigeki Tomita,Satsuki Kishikawa,Akane Hashizume,Toru Mitsumori,Hideaki Nitta,Hiroko Iizuka-Honma,Tomohiro Sawada,Mitsuo Okubo,Yasunobu Sekiguchi,Miki Ando,Masaaki NoguchiPubMed 37121717
- European journal of histochemistry : EJH 67:2023Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuhiko Hashimoto,Shunji Nishimura,Yu Shinyashiki,Tomohiko Ito,Ryosuke Kakinoki,Masao AkagiPubMed 37098880
- International journal of molecular medicine 51:2023STAT3/NF‑κB decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑κB signaling pathway in a mouse model of atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun-Jin An,Mi-Gyeong Gwon,Hyemin Gu,Seongjae Bae,Jaechan Leem,Jin Bae Lee,Kwan-Kyu ParkPubMed 37026512
- Arthritis research & therapy 25:282023MYC promotes fibroblast osteogenesis by regulating ALP and BMP2 to participate in ectopic ossification of ankylosing spondylitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianmei Jin,Yaoyang Liu,Zhiguo Zhang,Xingzhu Wen,Ziqiang Chen,Haijun Tian,Zijian Kang,Xin Wu,Huji XuPubMed 36803548
- Parasite immunology 45:e129712023Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen M Sandoval Pacheco,Gabriela V Araujo Flores,Aurea F Ferreira,Vânia L R da Matta,Claudia M de Castro Gomes,Wilfredo H Sosa-Ochoa,Concepción Zúniga,Fernando T Silveira,Carlos E P Corbett,Márcia D LaurentiPubMed 36695719
- Molecular pharmaceutics 19:4665-46742022Camptothesome Potentiates PD-L1 Immune Checkpoint Blockade for Improved Metastatic Triple-Negative Breast Cancer Immunochemotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiren Wang,Leyla Estrella Cordova,Pavani Chalasani,Jianqin LuPubMed 36413426
- Chinese medicine 17:1042022Weipiling decoction alleviates N-methyl-N-nitro-N'-nitrosoguanidine-induced gastric precancerous lesions via NF-κB signalling pathway inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPenghui Yang,Hongmei Yang,Hengli Zhou,Qiuyue Li,Sufen Wei,Qi Wang,Yan Yan,Yongqiang Liu,Huafeng Pan,Siyi LiPubMed 36085156
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IFNG. Database link P01579
Consider this alternative
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes Detects IFN-gamma by sandwich ELISA using 100μl/well antibody solution. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIFN-gamma. Can be paired for ELISA with ab9658. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
Recommended products
Rabbit Polyclonal Interferon gamma antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 104 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IFNG.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IFNG. Database link P01579
- Purification technique
- Affinity purification Protein A
- Light chain type
- unknown
- Reconstitution
- reconstitute with water at 200µL
- Concentration
- Purification notes
affinity chromatography employing immobilized Human IFN-gamma matrix.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see Anti-Interferon gamma antibody [EPR21704] ab231036.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Interferon gamma antibody (ab9657)
Lane 1: Western blot - Anti-Interferon gamma antibody (ab9657)
Lanes 2 - 12: Western blot - Anti-Interferon gamma antibody (ab9657) at 0.1 µg/mL
All lanes: Western blot - Recombinant human Interferon gamma protein (Active) (Recombinant human Interferon gamma protein (Active) ab9659)
Performed under non-reducing conditions.
Predicted band size: 19 kDa
Sandwich ELISA - Anti-Interferon gamma antibody (ab9657)
To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a compatible secondary detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com