AB231036

Anti-Interferon gamma antibody [EPR21704]

Name: Anti-Interferon gamma antibody [EPR21704] (ab231036) | Abcam
Brand: Abcam Limited
SKU: AB231036
Price: 190 USD
Availability: InStock
Rating: 4 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(22 Publications)

Anti-Interferon gamma antibody [EPR21704] (ab231036) is a rabbit monoclonal antibody detecting Interferon gamma in IHC-P, ELISA. Suitable for Human,.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (AB231036)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (AB231036)

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Paraffin embedded human tonsil tissue slides were dewaxed, followed by heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval ER2 Solution) for 20 mins. Endogenous peroxidase activity was blocked with 3% H2O2 for 10 mins. The sections were then incubated with ab231036 (1/3000) at room temperature for 30 mins, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The sections were counterstained with Hematoxylin. The images were taken with a Leica Aperio AT2.

ab231036 was stored at a range of temperatures (+4°C, +22°C, +37°C) for 1 week before being tested in IHC-P. The image shows the staining intensity remains relatively constant for temperatures ranging between 4°C, 22°C and 37°C, demonstrating that antibody activity is not affected under these conditions. This data was generated as part of a structured antibody stability study supporting Abcam’s transition from cold‑chain to ambient shipping.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (AB231036)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Sporadic cytoplasmic staining in limphocytes of human tonsil is observed (PMID : 21838712). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

ELISA - Anti-Interferon gamma antibody [EPR21704] (AB231036)

ELISA

Unknown

ELISA - Anti-Interferon gamma antibody [EPR21704] (AB231036)

ELISA analysis of Human IFN Gamma recombinant protein at 1000 ng/mL with ab231036. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Carrier free

Anti-Interferon gamma antibody [EPR21704] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Interferon gamma antibody [EPR21704]
Biotin

Biotin Anti-Interferon gamma antibody [EPR21704]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21704

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant fragment": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Interferon gamma antibody [EPR21704] (ab231036) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), ELISA in Human, samples.

Trusted by the scientific community
Anti-Interferon gamma [EPR21704] (ab231036) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21704] also available for your convenience: ab231036, Carrier free - ab231301, Carrier free - ab302859, Alexa Fluor® 594 - ab308599, Biotin - ab322914

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.

Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

See full target information IFNG

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

NPJ breast cancer 11:67 PubMed40624019

2025

NR4A3 potentials M1-like macrophage polarization to facilitate anti-tumor immune responses in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Yu Qian,Ning Jin,Shan-Shan Rao,Ya Wang,Xin Li,Wen Pan,Pu Huang,Si-Yuan Wang,Ping-Fei Li,Yan-Kai Lv,Qing-Lei Gao,Yu Xia

Cell death & disease 16:463 PubMed40541951

2025

ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ye,Genyi Jiang,Yihan Sun,Bilan Li

Communications biology 8:696 PubMed40319103

2025

Single-cell RNA sequencing reveals a fibroblast gene signature that promotes T-cell infiltration in muscle-invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zige Liu,Xingning Mao,Yuli Xie,Yunkun Yan,Xiang Wang,Junhao Mi,Hao Yuan,Jiange Zhang,Caisheng Huang,Jianxin Chen,Mujia Jili,Shengzhu Huang,Qingyun Zhang,Fubo Wang,Zengnan Mo,Rirong Yang

Nature communications 15:8690 PubMed39375367

2024

Single-cell RNA sequencing reveals the pro-inflammatory roles of liver-resident Th1-like cells in primary biliary cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ciliang Jin,Penglei Jiang,Zhaoru Zhang,Yingli Han,Xue Wen,Lin Zheng,Wei Kuang,Jiangshan Lian,Guodong Yu,Xinyue Qian,Yue Ren,Miaomiao Lu,Lingling Xu,Weixin Chen,Jiyang Chen,Yuwei Zhou,Jinxia Xin,Ben Wang,Xi Jin,Pengxu Qian,Yida Yang

iScience 27:110350 PubMed39108722

2024

Heterozygous Gnaq deficiency enhances Ifi202b/IFI16 and NF-κB activation in endothelial cells and exacerbates lupus nephritis pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Yan He,Mengqin Zhang,Jimin Zhang,Wuwei Zhuang,Yuechi Sun,Xing Chen,Hangzhou Fu,Xuanli Tang,Guixiu Shi

Nature communications 15:4698 PubMed38844770

2024

Ultrasound-mediated delivery of doxorubicin to the brain results in immune modulation and improved responses to PD-1 blockade in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor A Arrieta,Andrew Gould,Kwang-Soo Kim,Karl J Habashy,Crismita Dmello,Gustavo I Vázquez-Cervantes,Irina Palacín-Aliana,Graysen McManus,Christina Amidei,Cristal Gomez,Silpol Dhiantravan,Li Chen,Daniel Y Zhang,Ruth Saganty,Meghan E Cholak,Surya Pandey,Matthew McCord,Kathleen McCortney,Brandyn Castro,Rachel Ward,Miguel Muzzio,Guillaume Bouchoux,Carole Desseaux,Michael Canney,Alexandre Carpentier,Bin Zhang,Jason M Miska,Maciej S Lesniak,Craig M Horbinski,Rimas V Lukas,Roger Stupp,Catalina Lee-Chang,Adam M Sonabend

iScience 27:108779 PubMed38292420

2024

YTHDF2-mediated circYAP1 drives immune escape and cancer progression through activating YAP1/TCF4-PD-L1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuang Chen,Wenkang Wang,Shengyun Hu,Haifeng Sun,Chen Chen,Zhiyong Zhang,Xinzhi Sun,Bin Jia,Junhong Hu,Chengzeng Wang,Yang Liu,Zhenqiang Sun

iScience 26:107693 PubMed37680482

2023

Identification of S1PR4 as an immune modulator for favorable prognosis in HNSCC through machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Chenshen Huang,Fengshuo Zhu,Hao Zhang,Ning Wang,Qi Huang

Oncogenesis 12:31 PubMed37244923

2023

Integrated analysis of single-cell and bulk RNA sequencing data reveals an immunostimulatory microenvironment in tumor thrombus of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Ji,Qianyu Shi,Song Mei,Jiuhui Xu,Haijie Liang,Lu Xie,Tingting Ren,Kunkun Sun,Dasen Li,Xiaodong Tang,Peng Zhang,Wei Guo

Journal for immunotherapy of cancer 11: PubMed37072348

2023

Intratumoral tertiary lymphoid structure (TLS) maturation is influenced by draining lymph nodes of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miao He,Qihua He,Xiuyu Cai,Jun Liu,Hongshen Deng,Feng Li,Ran Zhong,Yi Lu,Haoxin Peng,Xiangrong Wu,Zisheng Chen,Shen Lao,Caichen Li,Jianfu Li,Jianxing He,Wenhua Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Interferon gamma antibody [EPR21704]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (AB231036)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (AB231036)

ELISA - Anti-Interferon gamma antibody [EPR21704] (AB231036)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

Product promise