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AB118262

Anti-Intersectin 1 antibody

3

(2 Reviews)

|

(6 Publications)

Rabbit Polyclonal Intersectin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITSN1 aa 600-850.

View Alternative Names

ITSN, SH3D1A, ITSN1, Intersectin-1, SH3 domain-containing protein 1A, SH3P17

1 Images
Western blot - Anti-Intersectin 1 antibody (AB118262)
  • WB

Unknown

Western blot - Anti-Intersectin 1 antibody (AB118262)

All lanes:

Western blot - Anti-Intersectin 1 antibody (ab118262) at 1/500 dilution

All lanes:

Human fetal brain lysate

Predicted band size: 195 kDa

Observed band size: 129 kDa,138 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ITSN1 aa 600-850. The exact immunogen used to generate this antibody is proprietary information.

Q15811

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intersectin 1 also known as ITS1 or intersectin is a multifunctional adaptor protein with a known mass of approximately 180 kDa. It plays a role in clathrin-mediated endocytosis by acting as a scaffold for the organization of various proteins involved in the process. Intersectin 1 is predominantly expressed in the brain but can also be found in other tissues like the heart and kidney. The presence of multiple SH3 domains and DH-PH domains facilitates its interaction with other proteins making it important for synaptic vesicle recycling.
Biological function summary

Intersectin 1 functions as a central hub within signal transduction pathways by forming multimeric complexes. It associates with proteins involved in endocytosis such as dynamin and synaptojanin and coordinates cellular responses to external signals. Its role in actin cytoskeleton dynamics supports its involvement in cell migration and mobility. These functions are essential for normal neurological development and synaptic function.

Pathways

Intersectin 1 integrates into signaling networks primarily within the endocytic and actin cytoskeleton regulatory pathways. It interacts with proteins like Cdc42 and N-WASP contributing to the remodeling of the cytoskeleton. In the receptor-mediated endocytosis pathway intersectin 1 regulates the internalization of membrane receptors influencing cellular communication and maintenance.

Intersectin 1 has connections to neurological and developmental issues including Down syndrome and autism spectrum disorders. This protein's interaction with amyloid precursor protein (APP) suggests a possible link to Alzheimer’s disease through altered APP processing. Additionally intersectin 1's dysregulation can affect pathways related to cell growth and differentiation contributing to cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed : 11584276, PubMed : 29887380). Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed : 11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (PubMed : 29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed : 22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1 (PubMed : 11744688).. Isoform 1. Plays a role in synaptic vesicle endocytosis in brain neurons.
See full target information ITSN1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35072626

2022

Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Ae Catsburg,Manon Westra,Annemarie Ml van Schaik,Harold D MacGillavry

OncoTargets and therapy 12:7079-7093 PubMed31564893

2019

Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xie,Wenmin Xiong,Junyu Li,Xia Wang,Chen Xu,Liping Yang

Nature communications 9:1960 PubMed29773874

2018

Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Shazia Ashraf,Hiroki Kudo,Jia Rao,Atsuo Kikuchi,Eugen Widmeier,Jennifer A Lawson,Weizhen Tan,Tobias Hermle,Jillian K Warejko,Shirlee Shril,Merlin Airik,Tilman Jobst-Schwan,Svjetlana Lovric,Daniela A Braun,Heon Yung Gee,David Schapiro,Amar J Majmundar,Carolin E Sadowski,Werner L Pabst,Ankana Daga,Amelie T van der Ven,Johanna M Schmidt,Boon Chuan Low,Anjali Bansal Gupta,Brajendra K Tripathi,Jenny Wong,Kirk Campbell,Kay Metcalfe,Denny Schanze,Tetsuya Niihori,Hiroshi Kaito,Kandai Nozu,Hiroyasu Tsukaguchi,Ryojiro Tanaka,Kiyoshi Hamahira,Yasuko Kobayashi,Takumi Takizawa,Ryo Funayama,Keiko Nakayama,Yoko Aoki,Naonori Kumagai,Kazumoto Iijima,Henry Fehrenbach,Jameela A Kari,Sherif El Desoky,Sawsan Jalalah,Radovan Bogdanovic,Nataša Stajić,Hildegard Zappel,Assel Rakhmetova,Sharon-Rose Wassmer,Therese Jungraithmayr,Juergen Strehlau,Aravind Selvin Kumar,Arvind Bagga,Neveen A Soliman,Shrikant M Mane,Lewis Kaufman,Douglas R Lowy,Mohamad A Jairajpuri,Richard P Lifton,York Pei,Martin Zenker,Shigeo Kure,Friedhelm Hildebrandt

Neuroscience 371:29-37 PubMed29199069

2017

VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Richter,Isabelle Schmutz,Mahesh Darna,Johannes-F Zander,Rohit Chavan,Urs Albrecht,Gudrun Ahnert-Hilger

Vascular pharmacology 74:60-72 PubMed26471833

2015

Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Hernández-García,M Luisa Iruela-Arispe,Guadalupe Reyes-Cruz,José Vázquez-Prado

Journal of cell science 127:673-85 PubMed24284073

2013

Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley C Humphries,Sara K Donnelly,Michael Way
View all publications
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