Rabbit Polyclonal Intersectin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Intersectin-1 aa 600-850.
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- eLife 11:2022Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Ae Catsburg,Manon Westra,Annemarie Ml van Schaik,Harold D MacGillavryPubMed 35072626
- OncoTargets and therapy 12:7079-70932019Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Xie et. al.PubMed 31564893
- Nature communications 9:19602018Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShazia Ashraf et. al.PubMed 29773874
- Neuroscience 371:29-372017VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarin Richter,Isabelle Schmutz,Mahesh Darna,Johannes-F Zander,Rohit Chavan,Urs Albrecht,Gudrun Ahnert-HilgerPubMed 29199069
- Vascular pharmacology 74:60-722015Endothelial RhoGEFs: A systematic analysis of their expression profiles in VEGF-stimulated and tumor endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Hernández-García,M Luisa Iruela-Arispe,Guadalupe Reyes-Cruz,José Vázquez-PradoPubMed 26471833
- Journal of cell science 127:673-852013Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshley C Humphries,Sara K Donnelly,Michael WayPubMed 24284073
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Intersectin-1 aa 600-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q15811
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:11584276, PubMed:29887380). Acts as a guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1 (PubMed:11744688).Isoform 1Plays a role in synaptic vesicle endocytosis in brain neurons.
Alternative names
ITSN, SH3D1A, ITSN, ITSN1, SH3D1A, Intersectin-1, SH3 domain-containing protein 1A, SH3P17
Recommended products
Rabbit Polyclonal Intersectin 1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Intersectin-1 aa 600-850.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Intersectin-1 aa 600-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q15811
- Purification technique
Affinity purification Immunogen
- Reconstitution
Reconstitute in 200 µL of water
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Intersectin 1 also known as ITS1 or intersectin is a multifunctional adaptor protein with a known mass of approximately 180 kDa. It plays a role in clathrin-mediated endocytosis by acting as a scaffold for the organization of various proteins involved in the process. Intersectin 1 is predominantly expressed in the brain but can also be found in other tissues like the heart and kidney. The presence of multiple SH3 domains and DH-PH domains facilitates its interaction with other proteins making it important for synaptic vesicle recycling.
- Biological function summary
Intersectin 1 functions as a central hub within signal transduction pathways by forming multimeric complexes. It associates with proteins involved in endocytosis such as dynamin and synaptojanin and coordinates cellular responses to external signals. Its role in actin cytoskeleton dynamics supports its involvement in cell migration and mobility. These functions are essential for normal neurological development and synaptic function.
- Pathways
Intersectin 1 integrates into signaling networks primarily within the endocytic and actin cytoskeleton regulatory pathways. It interacts with proteins like Cdc42 and N-WASP contributing to the remodeling of the cytoskeleton. In the receptor-mediated endocytosis pathway intersectin 1 regulates the internalization of membrane receptors influencing cellular communication and maintenance.
- Associated diseases and disorders
Intersectin 1 has connections to neurological and developmental issues including Down syndrome and autism spectrum disorders. This protein's interaction with amyloid precursor protein (APP) suggests a possible link to Alzheimer’s disease through altered APP processing. Additionally intersectin 1's dysregulation can affect pathways related to cell growth and differentiation contributing to cancer progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Intersectin 1 antibody (ab118262)
All lanes: Western blot - Anti-Intersectin 1 antibody (ab118262) at 1/500 dilution
All lanes: Human fetal brain lysate
Predicted band size: 195 kDa
Observed band size: 129 kDa, 138 kDa
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