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AB237509

Anti-Intersectin-2 antibody

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(1 Publication)

Rabbit Polyclonal Intersectin-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITSN2 aa 900 to C-terminus.

View Alternative Names

KIAA1256, SH3D1B, SWAP, ITSN2, Intersectin-2, SH3 domain-containing protein 1B, SH3P18, SH3P18-like WASP-associated protein

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Intersectin-2 antibody (AB237509)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Intersectin-2 antibody (AB237509)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Intersectin-2 using ab237509 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITSN2 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NZM3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intersectin-2 also known as ITSNT2 is a multi-domain scaffold protein involved in endocytosis and signal transduction. It has a molecular mass of approximately 194 kDa. Intersectin-2 helps form dynamic protein complexes by acting as an adaptor facilitating protein-protein interactions. This protein mainly expressed in the nervous system enabling synaptic vesicle recycling important for neurotransmission. It contains multiple EH (Eps15 Homology) domains SH3 (Src homology 3) domains and a coiled-coil domain all contributing to its versatile binding capabilities.
Biological function summary

The sophisticated molecular architecture of Intersectin-2 allows it to participate in essential cellular processes like endocytosis and actin cytoskeleton remodeling. It functions by interacting with proteins such as Dynamin Eps15 and Synaptojanin forming complexes necessary for clathrin-mediated endocytosis. Moreover its presence in the nervous system is significant for neuronal communication and plasticity by influencing synaptic vesicle trafficking and recycling. This activity highlights its role in modulating excitatory and inhibitory signals at synapses.

Pathways

Intersectin-2 plays an important role in the endocytic pathways and is a part of the Ras-MAPK signaling cascade. In the endocytic pathway Intersectin-2 interacts with several proteins like Clathrin and AP-2 complex facilitating vesicle formation and internalization of receptor-ligand complexes. In the Ras-MAPK signaling pathway it may regulate downstream signaling events by interacting with components such as SOS influencing cell growth and differentiation. These interactions link it to broader cellular processes like receptor-mediated signaling and cell survival.

Malfunction of Intersectin-2 links to neurological conditions such as Alzheimer's disease and epilepsy. In Alzheimer's disease altered Intersectin-2 expression correlates with dysfunctional endocytic activity and synaptic failure key features in disease progression. Furthermore in epilepsy interactions with proteins like Dynamin and Synaptojanin imply that disruptions in Intersectin-2's function may affect neuronal excitability and neurotransmitter release. Understanding these associations provides insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes (PubMed : 23999003).
See full target information ITSN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 11:2906-2918 PubMed32774771

2020

Biomarker analysis to predict the pathological response to neoadjuvant chemotherapy in locally advanced gastric cancer: An exploratory biomarker study of COMPASS, a randomized phase II trial.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Oshima,Takaki Yoshikawa,Yohei Miyagi,Satoshi Morita,Michio Yamamoto,Kazuaki Tanabe,Kazuhiro Nishikawa,Yuichi Ito,Takanori Matsui,Yutaka Kimura,Tomoyuki Yokose,Yukihiko Hiroshima,Toru Aoyama,Tsutomu Hayashi,Takashi Ogata,Haruhiko Cho,Yasushi Rino,Munetaka Masuda,Akira Tsuburaya,Junichi Sakamoto
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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