JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB262912

Anti-Intrinsic Factor antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal Intrinsic Factor antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CBLIF aa 150-350.

View Alternative Names

GIF, IFMH, CBLIF, Cobalamin binding intrinsic factor, Gastric intrinsic factor, Intrinsic factor, IF, INF

5 Images
Western blot - Anti-Intrinsic Factor antibody (AB262912)
  • WB

Unknown

Western blot - Anti-Intrinsic Factor antibody (AB262912)

All lanes:

Western blot - Anti-Intrinsic Factor antibody (ab262912) at 0.4 µg/mL

Lane 1:

Vector-only transfected HEK-293 cell lysate

Lane 2:

GIF overexpressing HEK-293 cell lysate

Predicted band size: 45 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)

Paraffin-embedded Human stomach tissue stained for Intrinsic Factor using ab262912 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)

Paraffin-embedded Human skin tissue stained for Intrinsic Factor using ab262912 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)

Paraffin-embedded Human kidney tissue stained for Intrinsic Factor using ab262912 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody (AB262912)

Paraffin-embedded Human liver tissue stained for Intrinsic Factor using ab262912 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CBLIF aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P27352

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50.00000 - 1/200.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intrinsic Factor also known as gastric intrinsic factor is a protein important for the absorption of vitamin B12. Produced by parietal cells in the gastric mucosa intrinsic factor has an approximate molecular weight of 45 kDa. It is secreted in the stomach and then travels to the small intestine. Intrinsic factor specifically binds to vitamin B12 forming a complex that enables the vitamin's uptake in the ileum. The presence of anti-intrinsic factor antibodies can impede its function playing a role in clinical diagnostics for conditions like pernicious anemia.
Biological function summary

The role of intrinsic factor is closely linked with vitamin B12 metabolism. As part of a transport complex it safeguards vitamin B12 from degradation as it moves through the gastrointestinal tract. In the absence of intrinsic factor vitamin B12 cannot effectively bind and undergo absorption in the ileum. The failure in this binding and absorption process often leads to severe nutritional deficiencies manifesting as a precursor to various hematological abnormalities.

Pathways

Intrinsic factor operates in the vitamin B12 absorption pathway. This pathway involves interactions with several proteins including cubilin and megalin which serve as part of the receptor-mediated endocytosis process in the ileum. Additionally the transcobalamin protein further facilitates the distribution of vitamin B12 within the bloodstream post-absorption highlighting the interconnected network between these proteins and intrinsic factor.

Intrinsic factor is strongly implicated in pernicious anemia. This condition arises when the body produces anti-intrinsic factor antibodies leading to vitamin B12 deficiency. The deficiency affects the production and maturation of red blood cells manifesting as macrocytic anemia. Moreover a lack of intrinsic factor has been associated with neurological disorders because vitamin B12 is critical for proper neural function. Without sufficient B12 myelin sheath formation and neuronal maintenance suffer which can lead to neurological complications.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.
See full target information CBLIF
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB181045

Anti-Intrinsic Factor antibody [EPR13488(B)]

primary-antibodies

intrinsic-factor-antibody-epr13488b-ab181045

0

(0 reviews)

Alternative Product
Primary Antibodies

AB221500

Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free

primary-antibodies

intrinsic-factor-antibody-epr13488b-bsa-and-azide-free-ab221500

0

(0 reviews)

Alternative Product
Primary Antibodies

AB102049

Anti-Intrinsic Factor antibody

primary-antibodies

intrinsic-factor-antibody-ab102049

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com