AB221500

Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Intrinsic Factor antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GIF, IFMH, CBLIF, Cobalamin binding intrinsic factor, Gastric intrinsic factor, Intrinsic factor, IF, INF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (AB221500)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (AB221500)

This data was developed using ab181045, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Intrinsic Factor using ab181045 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (AB221500)

This data was developed using ab181045, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Intrinsic Factor antibody [EPR13488(B)] (<a href='/en-us/products/primary-antibodies/intrinsic-factor-antibody-epr13488b-ab181045'>ab181045</a>) at 1/5000 dilution

All lanes:

Human stomach lysate at 10 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13488(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab221500 is the carrier-free version of ab181045.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intrinsic Factor also known as gastric intrinsic factor is a protein important for the absorption of vitamin B12. Produced by parietal cells in the gastric mucosa intrinsic factor has an approximate molecular weight of 45 kDa. It is secreted in the stomach and then travels to the small intestine. Intrinsic factor specifically binds to vitamin B12 forming a complex that enables the vitamin's uptake in the ileum. The presence of anti-intrinsic factor antibodies can impede its function playing a role in clinical diagnostics for conditions like pernicious anemia.

Biological function summary

The role of intrinsic factor is closely linked with vitamin B12 metabolism. As part of a transport complex it safeguards vitamin B12 from degradation as it moves through the gastrointestinal tract. In the absence of intrinsic factor vitamin B12 cannot effectively bind and undergo absorption in the ileum. The failure in this binding and absorption process often leads to severe nutritional deficiencies manifesting as a precursor to various hematological abnormalities.

Pathways

Intrinsic factor operates in the vitamin B12 absorption pathway. This pathway involves interactions with several proteins including cubilin and megalin which serve as part of the receptor-mediated endocytosis process in the ileum. Additionally the transcobalamin protein further facilitates the distribution of vitamin B12 within the bloodstream post-absorption highlighting the interconnected network between these proteins and intrinsic factor.

Intrinsic factor is strongly implicated in pernicious anemia. This condition arises when the body produces anti-intrinsic factor antibodies leading to vitamin B12 deficiency. The deficiency affects the production and maturation of red blood cells manifesting as macrocytic anemia. Moreover a lack of intrinsic factor has been associated with neurological disorders because vitamin B12 is critical for proper neural function. Without sufficient B12 myelin sheath formation and neuronal maintenance suffer which can lead to neurological complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.

See full target information CBLIF

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 8: PubMed32046307

2020

A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Artigas-Jerónimo,Juan J Pastor Comín,Margarita Villar,Marinela Contreras,Pilar Alberdi,Israel León Viera,Leandro Soto,Raúl Cordero,James J Valdés,Alejandro Cabezas-Cruz,Agustín Estrada-Peña,José de la Fuente

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (AB221500)

Western blot - Anti-Intrinsic Factor antibody [EPR13488(B)] - BSA and Azide free (AB221500)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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