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AB216263

Anti-Intron-encoded endonuclease I-SceI antibody

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(3 Publications)

Rabbit Polyclonal Intron-encoded endonuclease I-SceI antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.

View Alternative Names

OMEGA, SECY, Q0160, SCEI, Intron-encoded endonuclease I-SceI, 21S rRNA intron maturase, Homing endonuclease omega

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Intron-encoded endonuclease I-SceI antibody (AB216263)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Intron-encoded endonuclease I-SceI antibody (AB216263)

Immunofluorescent analysis U2OS cells transfected with GFP-SCE1 (Green) labeling SCE1 using ab216263 at 1/600 dilution (red). DAPI staining is showed and the merge figure too

Western blot - Anti-Intron-encoded endonuclease I-SceI antibody (AB216263)
  • WB

Supplier Data

Western blot - Anti-Intron-encoded endonuclease I-SceI antibody (AB216263)

All lanes:

Western blot - Anti-Intron-encoded endonuclease I-SceI antibody (ab216263) at 1/2000 dilution

Lane 1:

Non-transfected 293T cells lysate at 40 µg

Lane 2:

293T cells transfected with GFP-SCE1 lysate at 40 µg

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.

P03882

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Intron-encoded endonuclease I-SceI also known as endonuclease I-SceI or I-SceI endonuclease is a precise and highly specific endonuclease. It recognizes and cleaves an 18-base pair sequence. This sequence is rare resulting in the enzyme being used as a molecular tool for genome editing. I-SceI is encoded by a group I intron within the mitochondrial DNA of yeast and possesses a molecular mass of approximately 30 kDa. The enzyme expression is primarily observed in eukaryotic organisms often associated with mitochondrial genetic material.
Biological function summary

I-SceI facilitates homologous recombination and DNA repair by introducing double-strand breaks at specific genetic locations. Through this action it participates in gene conversion processes allowing for the correction of genetic sequences. It does not operate as a part of a larger protein complex; rather its function centers on individual action and recognition of its target sites. Biological effectiveness makes it an attractive candidate for genomic studies and manipulations.

Pathways

I-SceI contributes significantly to DNA repair pathways particularly homologous recombination. This pathway is important for maintaining genomic stability. I-SceI cooperates with proteins such as RAD51 which facilitates strand exchange during the DNA repair process. In another pathway I-SceI-induced breaks can spur the activation of the single-strand annealing pathway when homologous recombination is impaired showcasing its versatile role in DNA repair mechanisms.

I-SceI's role in DNA repair connects it to cancer research. Impaired DNA repair mechanisms can lead to genomic instability a contributing factor to tumorigenesis. By enabling targeted gene corrections I-SceI may present therapeutic potential in correcting cancer-causing mutations. Additionally its interaction with the RAD51 protein implicates it in conditions where DNA repair is compromised such as certain genetic disorders with defective recombination.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial DNA endonuclease involved in intron homing. It introduces a specific double-strand break in the DNA of the 21S rRNA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Specifically recognizes and cleaves the sequence 5'-TAGGGATAACAGGGTAAT-3'.
See full target information SCEI
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 48:10342-10352 PubMed32894284

2020

Hedgehog signaling enables repair of ribosomal DNA double-strand breaks.

Applications

WB

Species

Unspecified reactive species

Tshering D Lama-Sherpa,Victor T G Lin,Brandon J Metge,Shannon E Weeks,Dongquan Chen,Rajeev S Samant,Lalita A Shevde

The Journal of biological chemistry 293:12681-12689 PubMed29925587

2018

Mitochondrial accumulation of amyloid β (Aβ) peptides requires TOMM22 as a main Aβ receptor in yeast.

Applications

WB

Species

Unspecified reactive species

Wenxin Hu,Zhiming Wang,Hongjin Zheng

Nature communications 9:1016 PubMed29523790

2018

MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Tripathi,Himanshi Agarwal,Swati Priya,Harish Batra,Priyanka Modi,Monica Pandey,Dhurjhoti Saha,Sathees C Raghavan,Sagar Sengupta
View all publications
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Product promise

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