AB180934

Anti-INTS10 antibody [EPR13154]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal INTS10 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C8orf35, INTS10, Integrator complex subunit 10, Int10

1 Images

Supplier Data

Western blot - Anti-INTS10 antibody [EPR13154] (AB180934)

Blocking/dilution buffer and concentration 5% NFDM/TBST

All lanes:

Western blot - Anti-INTS10 antibody [EPR13154] (ab180934) at 1/1000 dilution

Lane 1:

HeLa cell line at 20 µg

Lane 2:

Jurkat cell line at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

false

Carrier free

Anti-INTS10 antibody [EPR13154] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13154

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INTS10 also known as Integrator Complex Subunit 10 is an integral component of the integrator complex which is involved in the transcriptional process. This protein has a molecular mass of approximately 110 kDa. It expresses widely across various tissues but shows higher expression in certain cell types suggesting a role in specific transcriptional functions. The integrator complex targets RNA polymerase II during the transcription of small nuclear RNAs (snRNAs) which are important for mRNA processing.

Biological function summary

Integrator Complex Subunit 10 contributes to the orchestration of snRNA maturation and termination joining other subunits to form a stable assembly. As part of the integrator complex INTS10 ensures the proper cleavage of snRNA transcripts working alongside partners within the complex. Its functions support RNA processing which is essential for gene expression regulation and cellular response to signals. INTS10 therefore plays a role in maintaining genomic stability through its activities within this complex.

Pathways

INTS10 participates in major transcriptional regulation pathways associated with RNA polymerase II. It influences the RNA processing pathway which is important for snRNA biogenesis. Through its relationship with other integrator subunits such as INTS11 and INTS12 INTS10 facilitates the timely termination of snRNA transcription. This regulation impacts gene expression and can affect pathways like RNA splicing ultimately influencing protein synthesis and cellular functions.

Alterations in INTS10 activity have been connected to neurodevelopmental disorders and certain cancer types. Disruption of the integrator complex often involving INTS11 affects RNA processing leading to transcriptional dysregulation implicated in neurodevelopmental abnormalities. Mutations or aberrant expression in components like INTS10 also influence pathways relevant to tumorigenesis indicating a role in cancer development and progression. Understanding INTS10's connections to these diseases aids in identifying potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope by different components of the INT complex (PubMed : 23904267).

See full target information INTS10

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106668 PubMed37168579

2023

Alterations of endogenous pain-modulatory system of the cerebral cortex in the neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Chen,Chen Wang,Qian Gong,Yihui Chai,Yunzhi Chen,Cuiwen Song,Yuanhua Wu,Long Wang

Cell systems 13:286-303.e10 PubMed35085500

2022

Sparse dictionary learning recovers pleiotropy from human cell fitness screens.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Pan,Jason J Kwon,Jessica A Talamas,Ashir A Borah,Francisca Vazquez,Jesse S Boehm,Aviad Tsherniak,Marinka Zitnik,James M McFarland,William C Hahn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-INTS10 antibody [EPR13154]

Western blot - Anti-INTS10 antibody [EPR13154] (AB180934)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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