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AB250267

Anti-INTS10 antibody [EPR13154] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal INTS10 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C8orf35, INTS10, Integrator complex subunit 10, Int10

1 Images
Western blot - Anti-INTS10 antibody [EPR13154] - BSA and Azide free (AB250267)
  • WB

Supplier Data

Western blot - Anti-INTS10 antibody [EPR13154] - BSA and Azide free (AB250267)

This data was developed using ab180934, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-INTS10 antibody [EPR13154] (<a href='/en-us/products/primary-antibodies/ints10-antibody-epr13154-ab180934'>ab180934</a>) at 1/1000 dilution

Lane 1:

HeLa cell line at 20 µg

Lane 2:

Jurkat cell line at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13154

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250267 is the carrier-free version of ab180934.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INTS10 also known as Integrator Complex Subunit 10 is an integral component of the integrator complex which is involved in the transcriptional process. This protein has a molecular mass of approximately 110 kDa. It expresses widely across various tissues but shows higher expression in certain cell types suggesting a role in specific transcriptional functions. The integrator complex targets RNA polymerase II during the transcription of small nuclear RNAs (snRNAs) which are important for mRNA processing.
Biological function summary

Integrator Complex Subunit 10 contributes to the orchestration of snRNA maturation and termination joining other subunits to form a stable assembly. As part of the integrator complex INTS10 ensures the proper cleavage of snRNA transcripts working alongside partners within the complex. Its functions support RNA processing which is essential for gene expression regulation and cellular response to signals. INTS10 therefore plays a role in maintaining genomic stability through its activities within this complex.

Pathways

INTS10 participates in major transcriptional regulation pathways associated with RNA polymerase II. It influences the RNA processing pathway which is important for snRNA biogenesis. Through its relationship with other integrator subunits such as INTS11 and INTS12 INTS10 facilitates the timely termination of snRNA transcription. This regulation impacts gene expression and can affect pathways like RNA splicing ultimately influencing protein synthesis and cellular functions.

Alterations in INTS10 activity have been connected to neurodevelopmental disorders and certain cancer types. Disruption of the integrator complex often involving INTS11 affects RNA processing leading to transcriptional dysregulation implicated in neurodevelopmental abnormalities. Mutations or aberrant expression in components like INTS10 also influence pathways relevant to tumorigenesis indicating a role in cancer development and progression. Understanding INTS10's connections to these diseases aids in identifying potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed : 38570683, PubMed : 38823386). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed : 38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed : 16239144, PubMed : 32647223). Within the integrator complex, INTS10 is part of the integrator tail module that acts as a platform for the recruitment of transcription factors at promoters (PubMed : 38823386). May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed : 23904267).
See full target information INTS10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
View all publications
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Product promise

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