AB315167

Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal INTS11 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

CPSF3L, RC68, INTS11, Integrator complex subunit 11, Int11, Cleavage and polyadenylation-specific factor 3-like protein, Protein related to CPSF subunits of 68 kDa, CPSF3-like protein, RC-68

2 Images

Supplier Data

Immunoprecipitation - Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free (AB315167)

This data was developed using ab315166, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

INTS11 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab315166 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315166 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab315166 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315166 in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-INTS11 antibody [EPR28317-52] (<a href='/en-us/products/primary-antibodies/ints11-antibody-epr28317-52-ab315166'>ab315166</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Supplier Data

Western blot - Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free (AB315167)

This data was developed using ab315166, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-INTS11 antibody [EPR28317-52] (<a href='/en-us/products/primary-antibodies/ints11-antibody-epr28317-52-ab315166'>ab315166</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 30 µg

Lane 2:

HeLa transfected with siRNA specifically targeting INTS11 whole cell lysate at 30 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 30 µg

Lane 5:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 6:

Mouse kidney tissue lysate at 30 µg

Lane 7:

Rat kidney tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 67 kDa,36 kDa

false

Exposure time: 180s

Unconjugated

Anti-INTS11 antibody [EPR28317-52]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28317-52

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab315167 is the carrier-free version of ab315166.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INTS11 also known as Integrator Complex Subunit 11 is an important component of the integrator complex involved in transcriptional regulation. It weighs approximately 52 kDa. INTS11 shows expression in various tissues with higher levels in testis brain and skeletal muscle. It acts as a subunit of the Integrator complex playing a role in the processing of small nuclear RNA (snRNA).

Biological function summary

The integrator complex containing INTS11 associates with the C-terminal domain of RNA polymerase II. This connection suggests its role in RNA processing events. INTS11 in collaboration with other subunits like INTS9 contributes to the cleavage and 3'-end processing of nascent snRNAs. The accurate processing of snRNA is essential for proper spliceosome formation and function impacting overall RNA maturation.

Pathways

INTS11 participates in RNA splicing and processing related pathways including the major spliceosomal pathway. It forms a vital axis with INTS9 another integrator complex subunit to assist in proper RNA transcription termination and processing. Its activity intersects with RNA polymerase II emphasizing its influence on RNA biosynthesis and turnover.

Mutations and dysregulation in INTS11 have been linked to neurological disorders and certain cancers. Disruption in its expression or function can lead to faulty RNA processing and splicing highlighting its impact on cellular homeostasis. Additionally INTS11 shows interaction with proteins like cyclin-dependent kinase CDK8 suggesting its regulatory effects on cell cycle and transcriptional control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA endonuclease component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed : 16239144, PubMed : 25201415, PubMed : 28396433, PubMed : 32697989, PubMed : 33243860, PubMed : 33548203, PubMed : 34762484, PubMed : 37080207, PubMed : 38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed : 32697989, PubMed : 33243860, PubMed : 33548203, PubMed : 34762484, PubMed : 37080207, PubMed : 38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed : 16239144, PubMed : 22252320, PubMed : 26308897, PubMed : 30737432). Within the integrator complex, INTS11 constitutes the RNA endonuclease subunit that degrades exiting nascent RNA transcripts (PubMed : 28396433, PubMed : 32697989, PubMed : 33243860, PubMed : 33548203, PubMed : 34762484, PubMed : 37080207, PubMed : 38570683). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed : 23904267).

See full target information INTS11

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free

Immunoprecipitation - Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free (AB315167)

Western blot - Anti-INTS11 antibody [EPR28317-52-] - BSA and Azide free (AB315167)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise