JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB234700

Anti-INTS9 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal INTS9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human INTS9 aa 350 to C-terminus.

View Alternative Names

RC74, INTS9, Integrator complex subunit 9, Int9, Protein related to CPSF subunits of 74 kDa, RC-74

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-INTS9 antibody (AB234700)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-INTS9 antibody (AB234700)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for INTS9 (green) using ab234700 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS9 antibody (AB234700)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS9 antibody (AB234700)

Paraffin-embedded human ovarian cancer tissue stained for INTS9 using ab234700 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS9 antibody (AB234700)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INTS9 antibody (AB234700)

Paraffin-embedded human prostate cancer tissue stained for INTS9 using ab234700 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-INTS9 antibody (AB234700)
  • WB

Supplier Data

Western blot - Anti-INTS9 antibody (AB234700)

All lanes:

Western blot - Anti-INTS9 antibody (ab234700) at 1/2000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 74 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human INTS9 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NV88

style
left-column

Complementary Products

Primary Antibodies

AB234744

Anti-YME1L1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YME1L1 antibody (AB234744)

  • 0
  • 0
Primary Antibodies

AB247258

Anti-ZAP70 antibody [YE291] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZAP70 antibody [YE291] - BSA and Azide free (AB247258)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 16
Primary Antibodies

AB318983

Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UNG2 antibody [EPR29004-78] - BSA and Azide free (AB318983)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Integrator Complex Subunit 9 commonly known as INTS9 plays a mechanical role in RNA polymerase II transcription. INTS9 has a molecular weight of approximately 110 kDa. It is expressed in various tissues with noticeable levels in the reproductive and immune systems. Researchers often refer to it by its alternate names such as Int9 or RC74. Its expression is not limited to a single cell type or tissue suggesting a broad functional role across the body.
Biological function summary

INTS9 forms part of the larger Integrator complex which interacts with the carboxy-terminal domain of RNA polymerase II. The complex is essential for processing small nuclear RNAs (snRNAs) influencing the transcription termination of snRNAs. INTS9 contributes to the regulation of transcription and RNA processing particularly in the modification of RNA molecules to make them functional. As a member of the Integrator complex it interacts with various subunits to ensure proper RNA polymerase II function.

Pathways

INTS9 is part of important cellular processes like transcription regulation and RNA processing. It plays a role in the snRNA-processing pathway where it ensures accurate transcription termination and RNA maturation. Proteins such as RNA polymerase II and other Integrator subunits like INTS11 work closely with INTS9 within these pathways. This coordination ensures the maintenance of cellular homeostasis and the accurate synthesis of RNA components necessary for cellular operations.

INTS9's role links to conditions that involve transcriptional dysregulation and RNA processing errors. Mutations in INTS9 have connections to neurological disorders as proper RNA processing is critical for neural function. Diseases like neurodevelopmental disorders show correlation with defective RNA transcription pathways involving RNA polymerase II. INTS9's interaction with proteins such as INTS11 and RNA polymerase II suggests a complex interplay where disruption could contribute to these transcription-related illnesses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed : 25201415, PubMed : 33243860, PubMed : 33548203, PubMed : 38570683). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed : 33243860, PubMed : 38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed : 16239144, PubMed : 22252320, PubMed : 26308897, PubMed : 30737432). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed : 23904267).
See full target information INTS9
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biotechnology 67:1628-1640 PubMed38771421

2024

Role of C/EBP Homologous Protein (CHOP) and Nupr1 Interaction in Endoplasmic Reticulum Stress-Induced Apoptosis of Lens Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Li,Junyi Liu,Yongying Tang,Hong Zhang,Yuanping Zhang,Xu Zha,Xueying Zhao
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com