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AB229243

Anti-INTU antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal INTU antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human INTU.

View Alternative Names

KIAA1284, PDZD6, PDZK6, INTU, Protein inturned, Inturned planar cell polarity effector homolog, PDZ domain-containing protein 6

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-INTU antibody - C-terminal (AB229243)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-INTU antibody - C-terminal (AB229243)

SK-N-SH cells stained for INTU (green) using ab229243 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Hoechst 33342 staining (blue).

Western blot - Anti-INTU antibody - C-terminal (AB229243)
  • WB

Supplier Data

Western blot - Anti-INTU antibody - C-terminal (AB229243)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-INTU antibody - C-terminal (ab229243) at 1/500 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 106 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human INTU. The exact immunogen used to generate this antibody is proprietary information.

Q9ULD6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INTU also known as Intu or Intu sg is an important component in developmental processes. Mechanical functions of this protein involve structural organization and modulation of cellular structures. INTU has a molecular weight of approximately 120 kDa. It displays expression predominantly in epithelial tissues where it plays a role in maintaining tissue morphology by regulating cellular architecture.
Biological function summary

INTU contributes to organizing cilia and managing cytoskeletal arrangement. INTU forms an integral part of the CPLANE complex which is essential for the proper functioning of a variety of cellular components. The activity of INTU in this complex directs ciliary assembly and positioning which are pivotal for signal transduction and cellular responses to environmental stimuli.

Pathways

INTU is an important player in the Hedgehog signaling pathway and planar cell polarity (PCP) pathway. Within the PCP pathway INTU interacts closely with the core PCP proteins which include Vangl2 and Fzd3 to regulate cell orientation and movement. In Hedgehog signaling INTU function is necessary for the transduction of signals that dictate developmental patterning and tissue differentiation across various organisms.

Mutations or malfunctions of INTU have been linked to ciliopathies such as Bardet-Biedl syndrome and neural tube defects. INTU-related anomalies disrupt normal ciliary function impacting ciliogenesis. The protein interacts with other ciliopathy-related proteins like BBS1 and OFD1 influencing ciliogenesis and related cellular processes leading to disease manifestations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the apical cell surface, which in turn is essential for the normal orientation of elongating ciliary microtubules. Plays a key role in definition of cell polarity via its role in ciliogenesis but not via conversion extension. Has an indirect effect on hedgehog signaling (By similarity). Proposed to function as core component of the CPLANE (ciliogenesis and planar polarity effectors) complex involved in the recruitment of peripheral IFT-A proteins to basal bodies (PubMed : 27158779). Required for recruitment of CPLANE2 to the mother centriole (By similarity). Binds phosphatidylinositol 3-phosphate with highest affinity, followed by phosphatidylinositol 4-phosphate and phosphatidylinositol 5-phosphate (By similarity).
See full target information INTU
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 14:7794-7823 PubMed36084949

2022

Multifaceted investigation underlies diverse mechanisms contributing to the downregulation of Hedgehog pathway-associated genes and in lung adenocarcinoma and uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Yin Edwin Chan,Zhefan Stephen Chen
View all publications
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