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AB234853

Anti-Inversin antibody

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(1 Publication)

Rabbit Polyclonal Inversin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human INVS aa 1-150.

View Alternative Names

INV, NPHP2, INVS, Inversin, Inversion of embryo turning homolog, Nephrocystin-2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Inversin antibody (AB234853)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Inversin antibody (AB234853)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Inversin (green) using ab234853 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inversin antibody (AB234853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inversin antibody (AB234853)

Paraffin-embedded human liver cancer tissue stained for Inversin using ab234853 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Inversin antibody (AB234853)
  • WB

Supplier Data

Western blot - Anti-Inversin antibody (AB234853)

All lanes:

Western blot - Anti-Inversin antibody (ab234853) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 118 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human INVS aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9Y283

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inversin also known as INV or INVS is a protein that plays a mechanical role in cellular signaling particularly in the process of cilia assembly and maintenance. It has a molecular weight of approximately 124 kDa. This protein is expressed in various tissues with notable abundance in the kidneys and other ciliated cells. Inversin is important for the function and structure of cells contributing to their ability to sense and respond to environmental cues.
Biological function summary

Inversin acts as an essential modulator of cell-cycle regulation and planar cell polarity. This protein is part of a complex that regulates the Wnt signaling pathway acting as a switch between canonical and non-canonical Wnt pathways. Inversin's role ensures the proper orientation and division of cells which is necessary for correct tissue development and function. In this way Inversin influences cell and organ structure and development.

Pathways

Inversin functions in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway it interacts with Dishevelled proteins modulating pathways to ensure proper signaling for development. In the Hedgehog pathway Inversin plays a role in coordinating signal transduction necessary for embryonic patterning and development. Proper engagement in these pathways ensures the correct relay of signals that influence numerous biological processes.

Inversin has been linked to nephronophthisis and situs inversus. Nephronophthisis a kidney disorder correlates with mutations in the inversin gene which disrupt cilia function. This dysfunction leads to kidney fibrosis and cyst formation. In addition Inversin's connection to situs inversus involves a misalignment in the left-right organization of internal organs highlighting its role in ensuring normal body asymmetry. In these diseases aberrations in proteins such as cystin and inversin contribute to improper cellular and organ function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for normal renal development and establishment of left-right axis. Probably acts as a molecular switch between different Wnt signaling pathways. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Involved in the organization of apical junctions in kidney cells together with NPHP1, NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity).
See full target information INVS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 17:2739-2759 PubMed36241724

2022

Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Ping Tan,Xiaodong Liu,Jose M Polo
View all publications
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