Rabbit Polyclonal Involucrin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications.
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- Science advances 9:eade25142023Engineering edgeless human skin with enhanced biomechanical properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pappalardo,David Alvarez Cespedes,Shuyang Fang,Abigail R Herschman,Eun Young Jeon,Kristin M Myers,Jeffrey W Kysar,Hasan Erbil AbaciPubMed 36706190
- Microbiology spectrum 10:e00681222022A Critical Role for p53 during the HPV16 Life Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian T Fontan,Claire D James,Apurva T Prabhakar,Molly L Bristol,Raymonde Otoa,Xu Wang,Elmira Karimi,Pavithra Rajagopalan,Devraj Basu,Iain M MorganPubMed 35608342
- mBio 12:e01163212021CK2 Phosphorylation of Human Papillomavirus 16 E2 on Serine 23 Promotes Interaction with TopBP1 and Is Critical for E2 Interaction with Mitotic Chromatin and the Viral Life Cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesApurva T Prabhakar,Claire D James,Dipon Das,Raymonde Otoa,Matthew Day,John Burgner,Christian T Fontan,Xu Wang,Sarah H Glass,Andreas Wieland,Mary M Donaldson,Molly L Bristol,Renfeng Li,Anthony W Oliver,Laurence H Pearl,Brian O Smith,Iain M MorganPubMed 34544280
- Nanomaterials (Basel, Switzerland) 10:2020Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenisse Esther Mallaupoma Camarena et. al.PubMed 33339410
- The Journal of investigative dermatology 140:2343-2352.e32020Air Particulate Matter Induces Skin Barrier Dysfunction and Water Transport Alteration on a Reconstructed Human Epidermis Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniele Seo Hieda et. al.PubMed 32339540
- Oncotarget 8:81892-819092017An oral keratinocyte life cycle model identifies novel host genome regulation by human papillomavirus 16 relevant to HPV positive head and neck cancer.
- Molecular therapy : the journal of the American So 22:725-332014Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant.
- PloS one 8:e524942013Keratinocytes propagated in serum-free, feeder-free culture conditions fail to form stratified epidermis in a reconstituted skin model.
- Pigment cell & melanoma research 25:354-692012Proteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla A Brohem et. al.PubMed 22372875
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
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Target data
Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Alternative names
Involucrin, IVL
Recommended products
Rabbit Polyclonal Involucrin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.
- Biological function summary
Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.
- Pathways
Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.
- Associated diseases and disorders
Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody (ab27495)
Paraffin embedded formalin fixed human skin tissue section stained with anti Involucrin (ab27495).
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