Rabbit Recombinant Monoclonal Involucrin antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 3 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (AB181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134637.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (AB181980)")
![Western blot - Anti-Involucrin antibody [EPR13054] (AB181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--western-blot-img144924.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Involucrin antibody [EPR13054] (AB181980)")
![Western blot - Anti-Involucrin antibody [EPR13054] (AB181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--western-blot-img147942.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Involucrin antibody [EPR13054] (AB181980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (AB181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132211.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (AB181980)")
![Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--flow-cytometry-intracellular-img54163.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (AB181980)")
Publications
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- Cell death discovery 8:1602022Smurf2-induced degradation of SMAD2 causes inhibition of hair follicle stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBojie Lin,Dan Huang,Guanyu Lin,Yong Miao,Jin Wang,Zhexiang Fan,Zhiqi HuPubMed 35379779
- ImmunoHorizons 5:830-8432021Transcriptomic Analysis of Healthy and Atopic Dermatitis Samples Reveals the Role of IL-37 in Human Skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiajun Zhou,David C Gemperline,Matthew J Turner,Jonathan Oldach,Jennifer Molignano,Jonathan T Sims,Keith R StayrookPubMed 34702760
- The Journal of biological chemistry 297:1012572021The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca R Miles,Parth H Amin,Miguel Barriera Diaz,Jagannath Misra,Erica Aukerman,Amitava Das,Nandini Ghosh,Tanner Guith,Michael D Knierman,Sashwati Roy,Dan F Spandau,Ronald C WekPubMed 34597669
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Tested | Expected | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10000 - 1/50000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Alternative names
Involucrin, IVL
Recommended products
Rabbit Recombinant Monoclonal Involucrin antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Rat, Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13054
- Purity
- Tissue culture supernatant
- Specificity
Unsuitable for rat IHC-P.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.
- Biological function summary
Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.
- Pathways
Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.
- Associated diseases and disorders
Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134637.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-Involucrin antibody [EPR13054] (ab181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--western-blot-img144924.jpg "Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)")
Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
Diluting and blocking buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-Involucrin antibody [EPR13054] (ab181980) at 1/500000 dilution
Lane 1: HaCaT (Human keratinocyte cell line) whole cell lysate at 10 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa
Exposure time: 1min
![Western blot - Anti-Involucrin antibody [EPR13054] (ab181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--western-blot-img147942.jpg "Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)")
Western blot - Anti-Involucrin antibody [EPR13054] (ab181980)
Diluting and blocking buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-Involucrin antibody [EPR13054] (ab181980) at 1/50000 dilution
Lane 1: Human fetal skin tissue at 10 µg
Lane 2: Rat skin tissue at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 120 kDa
Exposure time: 1min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132211.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [EPR13054] (ab181980)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Involucrin with ab181980 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980), expandable thumbnail](https://content.abcam.com/products/images/involucrin-antibody-epr13054-ab181980--flow-cytometry-intracellular-img54163.jpg "Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980)")
Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13054] (ab181980)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HUVEC cells labeling Involucrin with ab181980 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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