AB184969

Anti-Involucrin antibody [EPR13060(N)] - C-terminal

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Involucrin antibody. C-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Involucrin, IVL

3 Images

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Intracellular flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/10000 dilution

All lanes:

Human fetal skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

All lanes:

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution

All lanes:

HT-1080 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa

false

Carrier free

Anti-Involucrin antibody [EPR13060(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13060(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.

Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

See full target information IVL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:1365-1379.e22 PubMed31422906

2019

Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Kelsey N Lamb,Daniel Bsteh,Sarah N Dishman,Hagar F Moussa,Huitao Fan,Jacob I Stuckey,Jacqueline L Norris,Stephanie H Cholensky,Dongxu Li,Jingkui Wang,Cari Sagum,Benjamin Z Stanton,Mark T Bedford,Kenneth H Pearce,Terry P Kenakin,Dmitri B Kireev,Gang Greg Wang,Lindsey I James,Oliver Bell,Stephen V Frye

Journal of cellular biochemistry 120:9213-9229 PubMed30556162

2018

Peroxisome proliferator-activated receptor-α agonist and all-trans retinoic acid induce epithelial differentiation of subcutaneous adipose-derived stem cells from debrided burn skin.

Applications

Unspecified application

Species

Unspecified reactive species

Shanmugasundaram Natesan,Nicole L Wrice,Robert J Christy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Involucrin antibody [EPR13060(N)] - C-terminal

Flow Cytometry (Intracellular) - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Western blot - Anti-Involucrin antibody [EPR13060(N)] - C-terminal (AB184969)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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