AB227530

Anti-Involucrin antibody - N-terminal

Name: Anti-Involucrin antibody - N-terminal (ab227530) | Abcam
Brand: Abcam Limited
SKU: AB227530

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(1 Publication)

Rabbit Polyclonal Involucrin antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human IVL.

View Alternative Names

Involucrin, IVL

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (AB227530)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (AB227530)

Paraffin-embedded H661 xenograft stained for Involucrin with ab227530 (1/250 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody - N-terminal (AB227530)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody - N-terminal (AB227530)

A431 (human epidermoid carcinoma cell line) cells stained for Involucrin (green) using ab227530 (1/200 dilution) in ICC/IF. Cells were fixed in methanol.

Counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-Involucrin antibody - N-terminal (AB227530)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extracts at 30 µg

Lane 3:

NCI-H929 (human bone marrow lymphoblast cell line) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 68 kDa

true

Supplier Data

Western blot - Anti-Involucrin antibody - N-terminal (AB227530)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

Involucrin-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 68 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human IVL. The exact immunogen used to generate this antibody is proprietary information.

P07476

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.

Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

See full target information IVL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:113042 PubMed37651233

2023

Ectopic expression of DOCK8 regulates lysosome-mediated pancreatic tumor cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Omar L Gutierrez-Ruiz,Katherine M Johnson,Eugene W Krueger,Roseanne E Nooren,Nicole Cruz-Reyes,Carrie Jo Heppelmann,Tara L Hogenson,Martin E Fernandez-Zapico,Mark A McNiven,Gina L Razidlo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Involucrin antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (AB227530)

Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody - N-terminal (AB227530)

Western blot - Anti-Involucrin antibody - N-terminal (AB227530)

Western blot - Anti-Involucrin antibody - N-terminal (AB227530)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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