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AB20202

Anti-Involucrin antibody [SY8]

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(6 Publications)

Mouse Monoclonal Involucrin antibody. Suitable for IP, WB, IHC-P and reacts with Pig, Gorilla, Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human IVL.

View Alternative Names

Involucrin, IVL

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (AB20202)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody [SY8] (AB20202)

ab20202 staining Human skin Staining is localised to the cytoplasm.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SY8

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human, Pig, Gorilla

Applications

IP, WB, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human IVL.

P07476

Specificity

Keratinocytes of epidermis and other stratified squamous epithelia

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Involucrin also known as involucrine envocrin or ivrn is a protein with a mass of approximately 68 kDa. You will find involucrin expressed mainly in the suprabasal layers of the stratified squamous epithelia including epidermis tongue and esophagus. This protein becomes cross-linked to form the cornified cell envelope (CCE) in the upper layers of the skin an essential structure for epidermal differentiation and maintenance of skin barrier function.
Biological function summary

Involucrin serves as a critical substrate in the formation of the CCE by providing reactive sites for cross-linking with other proteins like loricrin and small proline-rich proteins. This cross-linking process occurs due to the action of transglutaminases which bond glutamine residues of involucrin with lysine residues in other proteins reinforcing the skin's protective barrier. Involucrin is a part of the protein network integral to keratinocyte terminal differentiation playing a distinctive role in the formation of a resilient and impermeable skin surface.

Pathways

Involucrin contributes directly within the epidermal differentiation complex (EDC) supporting the skin barrier's structural stability and resilience. The protein prominently acts in the terminal differentiation pathway in conjunction with other structural proteins such as filaggrin and keratins. This pathway forms a protective barrier that resists mechanical stress and environmental insults functioning together to provide a robust defense against external factors.

Involucrin's role is highlighted in conditions like psoriasis and ichthyosis where skin barrier formation is disrupted. Aberrant expression or modification of involucrin can lead to compromised barrier integrity leading to these skin disorders. It relates closely to filaggrin in these conditions as both are essential for barrier function and mutations in these proteins can exacerbate skin pathologies. Understanding these connections helps us evaluate potential therapeutic strategies for skin barrier-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
See full target information IVL
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 5:100357 PubMed40492178

2025

The Volume-Regulated Anion Channel LRRC8 is Involved in the Initiation of Epidermal Differentiation and is Deregulated in Psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Jahn,Victoria Lang,Oliver Rauh,Torsten Fauth,Claudia Buerger

Frontiers in bioengineering and biotechnology 12:1422235 PubMed39157442

2024

A multiparametric analysis including single-cell and subcellular feature assessment reveals differential behavior of spheroid cultures on distinct ultra-low attachment plate types.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Vitacolonna,Roman Bruch,Ane Agaçi,Elina Nürnberg,Tiziana Cesetti,Florian Keller,Francesco Padovani,Simeon Sauer,Kurt M Schmoller,Markus Reischl,Mathias Hafner,Rüdiger Rudolf

Cells 11: PubMed36139422

2022

mTORC1 Activity in Psoriatic Lesions Is Mediated by Aberrant Regulation through the Tuberous Sclerosis Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Ferreri,Victoria Lang,Roland Kaufmann,Claudia Buerger

Gene 842:146800 PubMed35961432

2022

Epigenetic remodeling of downstream enhancer regions is linked to selective expression of the IL1F10 gene in differentiated human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique Talabot-Ayer,Alejandro Diaz-Barreiro,Ali Modarressi,Gaby Palmer

Nitric oxide : biology and chemistry 87:31-42 PubMed30862476

2019

Increase of cystathionine-γ-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Itamar Goren,Yvette Köhler,Ahmed Aglan,Josef Pfeilschifter,Karl-Friedrich Beck,Stefan Frank

PloS one 12:e0180853 PubMed28700632

2017

Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation.

Applications

Unspecified application

Species

Human

Claudia Buerger,Nitesh Shirsath,Victoria Lang,Alina Berard,Sandra Diehl,Roland Kaufmann,Wolf-Henning Boehncke,Peter Wolf
View all publications
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Product promise

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