AB247334

Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GluA2, GLUR2, GRIA2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2

3 Images

Lab

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-3-antibody-ep929y-ab52896'>ab52896</a>) at 1/2000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 98 kDa

Observed band size: 100 kDa

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Unknown

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

All lanes:

Human brain tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

Lab

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 98 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP929Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects both GluR2 phosphorylated on Serine 880 + GluR3 phosphorylated on Serine 891 of the mature human, and also unphosphorylated GluR2 + 3.

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Reactivity data

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Product details

ab247334 is the carrier-free version of ab52896.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 and 3 also known as GluR2 and GluR3 are subunits of AMPA receptors which are ion channels facilitating synaptic transmission. These receptors bind glutamate an important neurotransmitter and become permeable to cations leading to rapid excitatory synaptic signaling. The combined molecular masses of GluR2 and GluR3 subunits are approximately 200 kDa. They are predominantly expressed in the central nervous system particularly in the cortex hippocampus and cerebellum where they contribute to fast synaptic transmission and plasticity.

Biological function summary

These receptors play a role in regulating synaptic strength and neuronal communication. They are part of AMPA receptor complexes vital for modulating synaptic plasticity that underlies learning and memory. GluR2 contains a Q/R site near pore region controlling calcium permeability and channel conductance. This editing is necessary to prevent excessive calcium entry ensuring regulated neuronal activity and maintaining neuroprotective functions.

Pathways

AMPA receptors including GluR2 and GluR3 integrate into the glutamatergic system and are significantly involved in the long-term potentiation (LTP) pathway essential for synaptic plasticity. They interact with proteins like NMDA receptors which help the initiation phase of LTP. They also play a role in excitotoxicity pathways where excessive glutamate can lead to neuronal damage. This interaction with glutamate saves as a bridge between normal synaptic activity and dysregulated excitatory signals.

Ionotropic glutamate receptor dysregulation associates with neurodegenerative diseases like Alzheimer’s disease and seizure disorders such as epilepsy. In Alzheimer's altered receptor trafficking leads to impaired synaptic function with amyloid-beta interacting with receptor systems including NMDA receptors. For epilepsy aberrant glutamate signaling through AMPA receptor subunits like GluR2 and GluR3 is a contributing factor to excitotoxicity and seizure pathogenesis suggesting potential therapeutic targets within receptor signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 20614889, PubMed : 31300657, PubMed : 8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed : 14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed : 20614889, PubMed : 8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

See full target information GRIA2

Additional targets

GRIA3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

Western blot - Anti-Ionotropic glutamate receptor 2 + 3 antibody [EP929Y] - BSA and Azide free (AB247334)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise