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AB247334

Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free

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Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

GluA2, GLUR2, GRIA2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2

4 Images
Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)
  • WB

Unknown

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-3-antibody-ep929y-ab52896'>ab52896</a>) at 1/5000 dilution

All lanes:

Human brain tissue lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)
  • WB

Lab

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-3-antibody-ep929y-ab52896'>ab52896</a>) at 1/2000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 98 kDa

Observed band size: 100 kDa

false

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)
  • WB

Lab

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-3-antibody-ep929y-ab52896'>ab52896</a>) at 1/10000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 98 kDa

Observed band size: 100 kDa

false

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)
  • WB

Lab

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] - BSA and Azide free (AB247334)

This data was developed using ab52896, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Ionotropic glutamate receptor 2 antibody [EP929Y] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-3-antibody-ep929y-ab52896'>ab52896</a>) at 1/5000 dilution

Lane 1:

293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with Human fragment GRIA2 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with Human full length GRIA3 expression vector containing a n-term myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human full length GRIA4 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

Recombinant Human full length GRIA1 Protein containing a C-Myc/DDK tag at 100 ng

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Lanes 1 - 5:

Goat anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution

Observed band size: 100 kDa

false

Exposure time: 20s

  • Unconjugated

    Anti-Ionotropic glutamate receptor 2 antibody [EP929Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP929Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247334 is the carrier-free version of ab52896.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 (GluR2) also known as GRIA2 plays an important role in synaptic transmission. It belongs to the family of ionotropic glutamate receptors and these receptors function as ligand-gated ion channels. GluR2 has a molecular weight of approximately 98 kDa. You will find GluR2 widely expressed in the central nervous system where it forms an essential part of excitatory neurotransmission processes. It influences how neurons respond to glutamate a principal excitatory neurotransmitter.
Biological function summary

Ion channels like GluR2 contribute to the regulation of synaptic plasticity. GluR2 often forms heteromeric complexes with other AMPA receptor subunits like GluR1 GluR3 and GluR4. These combinations determine the calcium permeability of the receptor complex which has implications for synaptic strength and plasticity. Such properties make GluR2 vital for memory formation and learning given its role in modifying synaptic responses based on neural activity.

Pathways

GluR2 engages significantly in synaptic signaling pathways. It takes part in the AMPA receptor trafficking pathway which dictates its movement to and from the synaptic membrane. GluR2 along with its related proteins like GRIA1 controls the flow of ions that are important for neurotransmission. Another important pathway involving GluR2 is the regulation of excitatory postsynaptic potentials. This function links GluR2 to various cellular processes that respond to neural activity changes.

GluR2 is linked to neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Mutations or malfunctions in GluR2-related pathways may lead to dysfunctional neurotransmission which can contribute to such conditions. Additionally abnormal GluR2 expression has connections to GRIA1 in epilepsy where changes in receptor composition affect neuronal excitability. Understanding GluR2 interactions in these contexts could provide valuable insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 20614889, PubMed : 31300657, PubMed : 8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed : 14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed : 20614889, PubMed : 8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
See full target information GRIA2

Additional targets

GRIA3
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Product promise

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