AB20673

Anti-Ionotropic Glutamate receptor 2 antibody

Name: Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) | Abcam
Brand: Abcam Limited
SKU: AB20673
Price: 465 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(38 Publications)

Rabbit Polyclonal Ionotropic Glutamate receptor 2 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 38 publications.

View Alternative Names

GluA2, Glur2, Gria2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2

2 Images

Unknown

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (AB20673)

The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/mL

All lanes:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 43 kDa,99 kDa

false

Project1059****

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (AB20673)

Additional bands : The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa.

Lane 1:

Marker

Lanes 2 - 5:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/mL

Lanes 2 and 4:

Brain (Mouse) Tissue Lysate at 10 µg

Lanes 3 and 5:

Cortex (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3-10 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3-10 µg/mL", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 (GluR2) also known as GRIA2 plays an important role in synaptic transmission. It belongs to the family of ionotropic glutamate receptors and these receptors function as ligand-gated ion channels. GluR2 has a molecular weight of approximately 98 kDa. You will find GluR2 widely expressed in the central nervous system where it forms an essential part of excitatory neurotransmission processes. It influences how neurons respond to glutamate a principal excitatory neurotransmitter.

Biological function summary

Ion channels like GluR2 contribute to the regulation of synaptic plasticity. GluR2 often forms heteromeric complexes with other AMPA receptor subunits like GluR1 GluR3 and GluR4. These combinations determine the calcium permeability of the receptor complex which has implications for synaptic strength and plasticity. Such properties make GluR2 vital for memory formation and learning given its role in modifying synaptic responses based on neural activity.

Pathways

GluR2 engages significantly in synaptic signaling pathways. It takes part in the AMPA receptor trafficking pathway which dictates its movement to and from the synaptic membrane. GluR2 along with its related proteins like GRIA1 controls the flow of ions that are important for neurotransmission. Another important pathway involving GluR2 is the regulation of excitatory postsynaptic potentials. This function links GluR2 to various cellular processes that respond to neural activity changes.

GluR2 is linked to neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Mutations or malfunctions in GluR2-related pathways may lead to dysfunctional neurotransmission which can contribute to such conditions. Additionally abnormal GluR2 expression has connections to GRIA1 in epilepsy where changes in receptor composition affect neuronal excitability. Understanding GluR2 interactions in these contexts could provide valuable insights into therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

See full target information Gria2

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 29:3607-3622 PubMed38822069

2024

The ion channel TRPA1 is a modulator of the cocaine reward circuit in the nucleus accumbens.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jung Kim,Su Jeong Choi,Sa-Ik Hong,Jung-Cheol Park,Youyoung Lee,Shi-Xun Ma,Kwang-Hyun Hur,Young Lee,Kyeong-Man Kim,Hyung Kyu Kim,Hee Young Kim,Seok-Yong Lee,Se-Young Choi,Choon-Gon Jang

Molecular neurodegeneration 18:65 PubMed37759260

2023

The Q/R editing site of AMPA receptor GluA2 subunit acts as an epigenetic switch regulating dendritic spines, neurodegeneration and cognitive deficits in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda L Wright,Lyndsey M Konen,Bruce G Mockett,Gary P Morris,Anurag Singh,Lisseth Estefania Burbano,Luke Milham,Monica Hoang,Raphael Zinn,Rose Chesworth,Richard P Tan,Gordon A Royle,Ian Clark,Steven Petrou,Wickliffe C Abraham,Bryce Vissel

Thoracic cancer 14:2897-2908 PubMed37605807

2023

AMPAR receptor inhibitors suppress proliferation of human small cell lung cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Nami Masumoto,Shingo Kato,Masahiro Aichi,Sho Hasegawa,Kota Sahara,Kumiko Suyama,Akane Sano,Tomoyuki Miyazaki,Koji Okudela,Takeshi Kaneko,Takuya Takahashi

NPJ Parkinson's disease 9:92 PubMed37328503

2023

Synaptic mechanisms underlying onset and progression of memory deficits caused by hippocampal and midbrain synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Attilio Iemolo,Maria De Risi,Nadia Giordano,Giulia Torromino,Cristina Somma,Diletta Cavezza,Martina Colucci,Maria Mancini,Antonio de Iure,Rocco Granata,Barbara Picconi,Paolo Calabresi,Elvira De Leonibus

Molecular neurobiology 60:3379-3395 PubMed36854997

2023

Caveolin-1-Mediated Cholesterol Accumulation Contributes to Exaggerated mGluR-Dependent Long-Term Depression and Impaired Cognition in Fmr1 Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Luo,Le Yang,Kun Zhang,Shi-Meng Zhou,Yan Wang,Liu-Kun Yang,Bin Feng,Shui-Bing Liu,Yu-Mei Wu,Ming-Gao Zhao,Qi Yang

Proceedings of the National Academy of Sciences of the United States of America 120:e2114747120 PubMed36716374

2023

CPEB3 low-complexity motif regulates local protein synthesis via protein-protein interactions in neuronal ribonucleoprotein granules.

Applications

Unspecified application

Species

Unspecified reactive species

Lenzie Ford,Arun Asok,Arielle D Tripp,Cameron Parro,Michelle Fitzpatrick,Christopher A de Solis,Po-Tao Y Chen,Neeva Shafiian,Luana Fioriti,Rajesh K Soni,Eric R Kandel

Psychopharmacology 240:239-248 PubMed36564670

2022

The M1 muscarinic acetylcholine receptor regulates the surface expression of the AMPA receptor subunit GluA2 via PICK1.

Applications

Unspecified application

Species

Unspecified reactive species

Zengyan Zhu,Wenjuan Wang,Chao Gu,Mei Wang,Yinghui Yan

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:7183-7200 PubMed35953295

2022

p140Cap Regulates the Composition and Localization of the NMDAR Complex in Synaptic Lipid Rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Costanza Angelini,Alessandro Morellato,Annalisa Alfieri,Lisa Pavinato,Tiziana Cravero,Olga Teresa Bianciotto,Vincenzo Salemme,Dora Natalini,Giorgia Centonze,Alessandra Raspanti,Tina Garofalo,Donatella Valdembri,Guido Serini,Andrea Marcantoni,Andrea Becchetti,Maurizio Giustetto,Emilia Turco,Paola Defilippi

Molecular brain 15:32 PubMed35387663

2022

Assessments of prolonged effects of desflurane and sevoflurane on motor learning deficits in aged App mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Niikura,Tomoyuki Miyazaki,Kenkichi Takase,Hiroki Sasaguri,Takashi Saito,Takaomi C Saido,Takahisa Goto

Cells 10: PubMed34943962

2021

The Novel Monoacylglycerol Lipase Inhibitor MJN110 Suppresses Neuroinflammation, Normalizes Synaptic Composition and Improves Behavioral Performance in the Repetitive Traumatic Brain Injury Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhuanand Selvaraj,Mikiei Tanaka,Jie Wen,Yumin Zhang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ionotropic Glutamate receptor 2 antibody

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (AB20673)

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (AB20673)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

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