AB52932

Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]

Name: Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) | Abcam
Brand: Abcam Limited
SKU: AB52932
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(11 Publications)

Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

GluA2, GLUR2, GRIA2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2

3 Images

Supplier Data

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/500 dilution

Lane 1:

Human brain

Lane 2:

Rat brain

Lane 3:

Mouse brain

Predicted band size: 98 kDa

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Unknown

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (ab52932) at 1/2000 dilution

All lanes:

Human brain cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 98 kDa

Observed band size: 110 kDa

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CiteAb

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

Ionotropic Glutamate receptor 2 western blot using anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] ab52932. Publication image and figure legend from Luo, Y. X., Xue, Y. X., et al., 2015, Nat Commun, PubMed 26169171.

ab52932 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab52932 please see the product overview.

Exposure to UCS decreased membrane expression of GluA1 and GluA2 in BLA.(a) Timeline of the experimental procedure. (b) Membrane expression of GluA1 and GluA2 proteins after the different experiment manipulations. Values are the mean percentage of protein levels in the BLA in reference to the saline+1 h+extinction group. One-way ANOVA, *Different from group 'saline+1 h+extinction', *P<0.05; n=6 per experimental condition. Error bars represent s.e.m. Uncropped western blots of the data depicted in Fig. 5 are provided in Supplementary Fig. 10.

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Carrier free

Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP966Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 (GluR2) also known as GRIA2 plays an important role in synaptic transmission. It belongs to the family of ionotropic glutamate receptors and these receptors function as ligand-gated ion channels. GluR2 has a molecular weight of approximately 98 kDa. You will find GluR2 widely expressed in the central nervous system where it forms an essential part of excitatory neurotransmission processes. It influences how neurons respond to glutamate a principal excitatory neurotransmitter.

Biological function summary

Ion channels like GluR2 contribute to the regulation of synaptic plasticity. GluR2 often forms heteromeric complexes with other AMPA receptor subunits like GluR1 GluR3 and GluR4. These combinations determine the calcium permeability of the receptor complex which has implications for synaptic strength and plasticity. Such properties make GluR2 vital for memory formation and learning given its role in modifying synaptic responses based on neural activity.

Pathways

GluR2 engages significantly in synaptic signaling pathways. It takes part in the AMPA receptor trafficking pathway which dictates its movement to and from the synaptic membrane. GluR2 along with its related proteins like GRIA1 controls the flow of ions that are important for neurotransmission. Another important pathway involving GluR2 is the regulation of excitatory postsynaptic potentials. This function links GluR2 to various cellular processes that respond to neural activity changes.

GluR2 is linked to neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Mutations or malfunctions in GluR2-related pathways may lead to dysfunctional neurotransmission which can contribute to such conditions. Additionally abnormal GluR2 expression has connections to GRIA1 in epilepsy where changes in receptor composition affect neuronal excitability. Understanding GluR2 interactions in these contexts could provide valuable insights into therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 20614889, PubMed : 31300657, PubMed : 8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed : 14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed : 20614889, PubMed : 8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

See full target information GRIA2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7971 PubMed38042894

2023

Enhanced TARP-γ8-PSD-95 coupling in excitatory neurons contributes to the rapid antidepressant-like action of ketamine in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Ge Xue,Jin-Gang He,Ling-Li Lu,Shi-Jie Song,Mei-Mei Chen,Fang Wang,Jian-Guo Chen

Brain, behavior, and immunity 108:204-220 PubMed36496170

2022

Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals.

Applications

Unspecified application

Species

Unspecified reactive species

Xia-Ping Yao,Jian Ye,Ting Feng,Feng-Chao Jiang,Ping Zhou,Fang Wang,Jian-Guo Chen,Peng-Fei Wu

Neurobiology of stress 18:100453 PubMed35685681

2022

Repeated vagus nerve stimulation produces anxiolytic effects via upregulation of AMPAR function in centrolateral amygdala of male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Qi Zhang,Zhi-Xuan Xia,Qiao Deng,Ping-Fen Yang,Li-Hong Long,Fang Wang,Jian-Guo Chen

Biochemical pharmacology 180:114136 PubMed32628930

2020

Oridonin is an antidepressant molecule working through the PPAR-γ/AMPA receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Liu,Jing Du

Molecular psychiatry 22:1274-1281 PubMed28831198

2017

Methamphetamine abuse impairs motor cortical plasticity and function.

Applications

Unspecified application

Species

Unspecified reactive species

X Huang,Y-Y Chen,Y Shen,X Cao,A Li,Q Liu,Z Li,L-B Zhang,W Dai,T Tan,O Arias-Carrion,Y-X Xue,H Su,T-F Yuan

Synapse (New York, N.Y.) 70:471-4 PubMed27262028

2016

Differential expression of postsynaptic NMDA and AMPA receptor subunits in the hippocampus and prefrontal cortex of the flinders sensitive line rat model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Treccani,Kristian Gaarn du Jardin,Gregers Wegener,Heidi Kaastrup Müller

Neuropsychopharmacology : official publication of 41:2541-56 PubMed27103064

2016

EphB2 in the Medial Prefrontal Cortex Regulates Vulnerability to Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Xi Zhang,Ying Han,Chen Chen,Ling-Zhi Xu,Jia-Li Li,Na Chen,Cheng-Yu Sun,Wen-Hao Chen,Wei-Li Zhu,Jie Shi,Lin Lu

Scientific reports 6:22096 PubMed26926225

2016

Epigenetic modification of PKMζ rescues aging-related cognitive impairment.

Applications

Species

Rat

Chen Chen,Shi-Qiu Meng,Yan-Xue Xue,Ying Han,Cheng-Yu Sun,Jia-Hui Deng,Na Chen,Yan-Ping Bao,Fei-Long Zhang,Lin-Lin Cao,Wei-Guo Zhu,Jie Shi,Wei-Hong Song,Lin Lu

Nature communications 6:7675 PubMed26169171

2015

A novel UCS memory retrieval-extinction procedure to inhibit relapse to drug seeking.

Applications

Species

Rat

Yi-xiao Luo,Yan-xue Xue,Jian-feng Liu,Hai-shui Shi,Min Jian,Ying Han,Wei-li Zhu,Yan-ping Bao,Ping Wu,Zeng-bo Ding,Hao-wei Shen,Jie Shi,Yavin Shaham,Lin Lu

Neuropsychopharmacology : official publication of 40:2146-56 PubMed25722116

2015

Overexpression of Protein Kinase Mζ in the Prelimbic Cortex Enhances the Formation of Long-Term Fear Memory.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Xue Xue,Zhen-Zhen Zhu,Hai-Bin Han,Jian-Feng Liu,Shi-Qiu Meng,Chen Chen,Jian-Li Yang,Ping Wu,Lin Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y]

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EP966Y] (AB52932)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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