Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (AB206293)

Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 + 3 with ab206293 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on Human solitary fibrous tumor is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (AB206293)

Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : C6 whole cell lysate 10μg (Input).

Lane 2 : ab206293 IP in C6 whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (ab206293)

Predicted band size: 98 kDa

false

• WB

Supplier Data

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (AB206293)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 second; Lane 2,3,4,5,6 and 7 : 10 seconds.

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (ab206293) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 6:

Human fetal kidney tissue lysate at 10 µg

Lane 7:

HT1080 (Human fibrosarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 99 kDa

false

• WB

Supplier Data

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (AB206293)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (ab206293) at 1/2000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 5:

Human fetal kidney tissue lysate at 20 µg

Lane 6:

HT1080 (Human fibrosarcoma cell line) whole cell lysate at 20 µg

Lane 7:

Mouse heart tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Lane 9:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 99 kDa

false

Exposure time: 3min