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AB251444

Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free

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Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Rat, Mouse, Human samples.

View Alternative Names

GluA2, GLUR2, GRIA2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, GluA3, GLUR3, GLURC, GRIA3, Glutamate receptor 3, GluR-3, AMPA-selective glutamate receptor 3, GluR-C, GluR-K3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)

This data was developed using ab206293, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 + 3 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on Human solitary fibrous tumor is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)
  • IP

Supplier Data

Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)

This data was developed using ab206293, the same antibody clone in a different buffer formulation.

Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : C6 whole cell lysate 10μg (Input).
Lane 2 : ab206293 IP in C6 whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-antibody-epr18115-ab206293'>ab206293</a>)

Predicted band size: 98 kDa

false

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)
  • WB

Supplier Data

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)

This data was developed using ab206293, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 1 second; Lanes 2-7 : 10 seconds.

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-antibody-epr18115-ab206293'>ab206293</a>) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 6:

Human fetal kidney tissue lysate at 10 µg

Lane 7:

HT1080 (Human fibrosarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 99 kDa

false

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)
  • WB

Supplier Data

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] - BSA and Azide free (AB251444)

This data was developed using ab206293, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (<a href='/en-us/products/primary-antibodies/ionotropic-glutamate-receptor-2-antibody-epr18115-ab206293'>ab206293</a>) at 1/2000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 5:

Human fetal kidney tissue lysate at 20 µg

Lane 6:

HT1080 (Human fibrosarcoma cell line) whole cell lysate at 20 µg

Lane 7:

Mouse heart tissue lysate at 20 µg

Lane 8:

Rat spleen tissue lysate at 20 µg

Lane 9:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 98 kDa

Observed band size: 99 kDa

false

Exposure time: 3min

  • Unconjugated

    Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18115

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>IHC is recommended for human only.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab251444 is the carrier-free version of ab206293.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 and 3 also known as GluR2 and GluR3 are subunits of AMPA receptors which are ion channels facilitating synaptic transmission. These receptors bind glutamate an important neurotransmitter and become permeable to cations leading to rapid excitatory synaptic signaling. The combined molecular masses of GluR2 and GluR3 subunits are approximately 200 kDa. They are predominantly expressed in the central nervous system particularly in the cortex hippocampus and cerebellum where they contribute to fast synaptic transmission and plasticity.
Biological function summary

These receptors play a role in regulating synaptic strength and neuronal communication. They are part of AMPA receptor complexes vital for modulating synaptic plasticity that underlies learning and memory. GluR2 contains a Q/R site near pore region controlling calcium permeability and channel conductance. This editing is necessary to prevent excessive calcium entry ensuring regulated neuronal activity and maintaining neuroprotective functions.

Pathways

AMPA receptors including GluR2 and GluR3 integrate into the glutamatergic system and are significantly involved in the long-term potentiation (LTP) pathway essential for synaptic plasticity. They interact with proteins like NMDA receptors which help the initiation phase of LTP. They also play a role in excitotoxicity pathways where excessive glutamate can lead to neuronal damage. This interaction with glutamate saves as a bridge between normal synaptic activity and dysregulated excitatory signals.

Ionotropic glutamate receptor dysregulation associates with neurodegenerative diseases like Alzheimer’s disease and seizure disorders such as epilepsy. In Alzheimer's altered receptor trafficking leads to impaired synaptic function with amyloid-beta interacting with receptor systems including NMDA receptors. For epilepsy aberrant glutamate signaling through AMPA receptor subunits like GluR2 and GluR3 is a contributing factor to excitotoxicity and seizure pathogenesis suggesting potential therapeutic targets within receptor signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 20614889, PubMed : 31300657, PubMed : 8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed : 14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed : 20614889, PubMed : 8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
See full target information GRIA2

Additional targets

GRIA3
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Product promise

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For full details, please see our Terms & Conditions

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