Skip to main content

Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Mouse, Human, Rat samples. Cited in 31 publications.


Images

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)WBIHC-P
Human
Expected
Tested
Tested
Not recommended
Mouse
Tested
Expected
Expected
Not recommended
Rat
Expected
Expected
Expected
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/70

Notes

-

Expected
Expected

Species

Human

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/70

Notes

-

Tested
Tested

Species

Human

Dilution info

1/80

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/80

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system (PubMed:31300657). It plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).

Alternative names

Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, GluA2, GRIA2, GLUR2

Recommended products

Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Mouse, Human, Rat samples. Cited in 31 publications.

Alternative names

Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, GluA2, GRIA2, GLUR2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5032

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ionotropic glutamate receptor 2 (GluR2) also known as GRIA2 plays an important role in synaptic transmission. It belongs to the family of ionotropic glutamate receptors and these receptors function as ligand-gated ion channels. GluR2 has a molecular weight of approximately 98 kDa. You will find GluR2 widely expressed in the central nervous system where it forms an essential part of excitatory neurotransmission processes. It influences how neurons respond to glutamate a principal excitatory neurotransmitter.

Biological function summary

Ion channels like GluR2 contribute to the regulation of synaptic plasticity. GluR2 often forms heteromeric complexes with other AMPA receptor subunits like GluR1 GluR3 and GluR4. These combinations determine the calcium permeability of the receptor complex which has implications for synaptic strength and plasticity. Such properties make GluR2 vital for memory formation and learning given its role in modifying synaptic responses based on neural activity.

Pathways

GluR2 engages significantly in synaptic signaling pathways. It takes part in the AMPA receptor trafficking pathway which dictates its movement to and from the synaptic membrane. GluR2 along with its related proteins like GRIA1 controls the flow of ions that are important for neurotransmission. Another important pathway involving GluR2 is the regulation of excitatory postsynaptic potentials. This function links GluR2 to various cellular processes that respond to neural activity changes.

Associated diseases and disorders

GluR2 is linked to neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Mutations or malfunctions in GluR2-related pathways may lead to dysfunctional neurotransmission which can contribute to such conditions. Additionally abnormal GluR2 expression has connections to GRIA1 in epilepsy where changes in receptor composition affect neuronal excitability. Understanding GluR2 interactions in these contexts could provide valuable insights into therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477), expandable thumbnail

    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

    All lanes: Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/5000 dilution

    Lane 1: Human hypothalamus lysate at 15 µg

    Lane 2: Human midbrain lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 98 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Ionotropic Glutamate receptor 2 with purified ab133477 at 1/70 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com