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AB133477

Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

5

(3 Reviews)

|

(31 Publications)

Rabbit Recombinant Monoclonal Ionotropic Glutamate receptor 2 antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Mouse, Human, Rat samples. Cited in 31 publications.

View Alternative Names

GluA2, GLUR2, GRIA2, Glutamate receptor 2, GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)

Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Ionotropic Glutamate receptor 2 with purified ab133477 at 1/70 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)
  • WB

Unknown

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (AB133477)

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/5000 dilution

Lane 1:

Human hypothalamus lysate at 15 µg

Lane 2:

Human midbrain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 98 kDa

false

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

  • Carrier free

    Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5032

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/70", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/70", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ionotropic glutamate receptor 2 (GluR2) also known as GRIA2 plays an important role in synaptic transmission. It belongs to the family of ionotropic glutamate receptors and these receptors function as ligand-gated ion channels. GluR2 has a molecular weight of approximately 98 kDa. You will find GluR2 widely expressed in the central nervous system where it forms an essential part of excitatory neurotransmission processes. It influences how neurons respond to glutamate a principal excitatory neurotransmitter.
Biological function summary

Ion channels like GluR2 contribute to the regulation of synaptic plasticity. GluR2 often forms heteromeric complexes with other AMPA receptor subunits like GluR1 GluR3 and GluR4. These combinations determine the calcium permeability of the receptor complex which has implications for synaptic strength and plasticity. Such properties make GluR2 vital for memory formation and learning given its role in modifying synaptic responses based on neural activity.

Pathways

GluR2 engages significantly in synaptic signaling pathways. It takes part in the AMPA receptor trafficking pathway which dictates its movement to and from the synaptic membrane. GluR2 along with its related proteins like GRIA1 controls the flow of ions that are important for neurotransmission. Another important pathway involving GluR2 is the regulation of excitatory postsynaptic potentials. This function links GluR2 to various cellular processes that respond to neural activity changes.

GluR2 is linked to neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Mutations or malfunctions in GluR2-related pathways may lead to dysfunctional neurotransmission which can contribute to such conditions. Additionally abnormal GluR2 expression has connections to GRIA1 in epilepsy where changes in receptor composition affect neuronal excitability. Understanding GluR2 interactions in these contexts could provide valuable insights into therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed : 20614889, PubMed : 31300657, PubMed : 8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed : 14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed : 20614889, PubMed : 8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
See full target information GRIA2
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Publications (31)

Recent publications for all applications. Explore the full list and refine your search

Molecular pain 19:17448069231222406 PubMed38073552

2023

A-kinase anchoring protein 79/150 coordinates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor sensitization in sensory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Nathaniel A Jeske

Journal of translational medicine 21:567 PubMed37620837

2023

Inhibiting NLRP3 inflammasome signaling pathway promotes neurological recovery following hypoxic-ischemic brain damage by increasing p97-mediated surface GluA1-containing AMPA receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Chen,Xiaohuan Li,Qian Xiong,Yehong Du,Man Luo,Lilin Yi,Yayan Pang,Xiuyu Shi,Yu Tian Wang,Zhifang Dong

Archives of toxicology 97:2371-2383 PubMed37482551

2023

Chronic exposure to inorganic arsenic and fluoride induces redox imbalance, inhibits the transsulfuration pathway, and alters glutamate receptor expression in the brain, resulting in memory impairment in adult male mouse offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy L González-Alfonso,Petrosyan Pavel,Hernández-Mercado Karina,Luz M Del Razo,Luz C Sanchez-Peña,Angélica Zepeda,María E Gonsebatt

JCI insight 8: PubMed37192007

2023

MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei Zhu

Cells 12: PubMed37174668

2023

Cognitive Impairment Is Associated with AMPAR Glutamatergic Dysfunction in a Mouse Model of Neuronal Methionine Synthase Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ziad Hassan,David Coelho,Carine Bossenmeyer-Pourié,Karim Matmat,Carole Arnold,Aurélie Savladori,Jean-Marc Alberto,Rémy Umoret,Jean-Louis Guéant,Grégory Pourié

Translational neurodegeneration 12:1 PubMed36624510

2023

p85S6K sustains synaptic GluA1 to ameliorate cognitive deficits in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Bing Li,Xiao-Yu Hu,Mu-Wen Chen,Cai-Hong Xiong,Na Zhao,Yan-Hui Ge,Hao Wang,Xiao-Ling Gao,Nan-Jie Xu,Lan-Xue Zhao,Zhi-Hua Yu,Hong-Zhuan Chen,Yu Qiu

Current research in toxicology 3:100093 PubMed36393872

2022

Developmental deltamethrin: Sex-specific hippocampal effects in Sprague Dawley rats.

Applications

Unspecified application

Species

Unspecified reactive species

Emily M Pitzer,Chiho Sugimoto,Samantha L Regan,Gary A Gudelsky,Michael T Williams,Charles V Vorhees

BMC psychiatry 22:182 PubMed35291971

2022

BDNF-TrkB signaling-mediated upregulation of Narp is involved in the antidepressant-like effects of (2R,6R)-hydroxynorketamine in a chronic restraint stress mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Lingsha Ju,Jiaojiao Yang,Tingting Zhu,Panmiao Liu,Jianjun Yang

International journal of molecular sciences 23: PubMed35216162

2022

Impaired Functional Connectivity Underlies Fragile X Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Lital Gildin,Rossana Rauti,Ofir Vardi,Liron Kuznitsov-Yanovsky,Ben M Maoz,Menahem Segal,Dalit Ben-Yosef

Journal of clinical laboratory analysis 36:e24196 PubMed34997978

2022

Proline-rich transmembrane protein 2 specifically binds to GluA1 but has no effect on AMPA receptor-mediated synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Yang Feng,Fengchang Qiao,Jianxin Tan,Xiaozuo Zhang,Ping Hu,Yun Stone Shi,Zhengfeng Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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