Anti-Ionotropic Glutamate receptor 2 antibody [L21/32]

• IHC-P

Ap

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [L21/32] (AB192760)

IHC image of Ionotropic Glutamate receptor 2 [L21/32] staining in Human Normal Cerebellum formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab192760, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Lab

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [L21/32] (AB192760)

All lanes:

Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [L21/32] (ab192760) at 1 µg/mL

Lane 1:

Mouse Cortex Tissue Lysate

Lane 2:

Rat Cortex Tissue Lysate at 20 µg

Lane 3:

Brain: cerebral cortex (Human) Cytoplasmic Lysate - adult normal tissue at 20 µg

Lane 4:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 20 µg

Lane 5:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/50000 dilution

Predicted band size: 98 kDa

Observed band size: 98 kDa

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Exposure time: 4min