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AB307997

Anti-IP-10 antibody [RP23040081]

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Rabbit Recombinant Multiclonal IP10 antibody. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-IP-10 antibody [RP23040081] (AB307997)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IP-10 antibody [RP23040081] (AB307997)

For immunofluorescence analysis, 70% confluent log phase HT-29 cells and HT-29 cells treated with 50 ng/mL IFN gamma for 18h followed by 1x PTI treatment for 4h were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ab307997 at 1/200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 at 1/2500 dilution, for 45 minutes at room temperature (Panel a : Green). Nuclei (Panel b : Blue) were stained with DAPI F-actin (Panel c : Red) was stained with Rhodamine Phalloidin at 1/300 dilution Panel d represents the merged image showing Cytosolic localization Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040081

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP-10 also known as CXCL10 is a chemokine with a molecular weight of approximately 8.7 kDa. It functions by binding to the CXCR3 receptor a process that regulates immune responses. IP-10 is expressed in a variety of cells such as monocytes epithelial cells and fibroblasts particularly during inflammatory states. Its expression occurs in response to interferons especially IFN-γ indicating its role in immune system activity.
Biological function summary

The protein functions in the recruitment of immune cells like activated T-cells and NK cells to sites of inflammation or infection. It acts independently and is not known to be part of a larger protein complex. IP-10 plays an important role in promoting inflammation thereby contributing to the host defense mechanisms against microbial pathogens. It participates actively in immune surveillance and responses to various pathogens by attracting the immune cells to where they are needed.

Pathways

The chemokine is integral to the inflammatory response and cellular immune response pathways. Throughout these pathways IP-10 operates alongside various cytokines and chemokines such as CXCL9 and CXCL11 which also bind to the CXCR3 receptor. These proteins together modulate leukocyte movement and activities leading to immunity against pathogens as well as regulation of inflammatory processes.

IP-10 has connections with multiple sclerosis and hepatitis C. In multiple sclerosis elevated IP-10 levels correlate with disease severity and progression involving interactions with other chemokines like MCP-1. For hepatitis C IP-10 helps in mediating liver inflammation and fibrosis linking its activity closely with interleukins such as IL-10. Its elevation in these diseases makes IP-10 a potential biomarker for monitoring disease progression and response to therapy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed : 11157474, PubMed : 22652417, PubMed : 7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed : 12750173, PubMed : 19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed : 12663757, PubMed : 12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
See full target information CXCL10
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Product promise

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For full details, please see our Terms & Conditions

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